Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

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O87125 (CHEB1_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	PA1459

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099 Required for aerobic and anaerobic chemotaxis. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H₂O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 368

368

Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099

PRO_0000158008

Regions

Domain

4 – 121

118

Response regulatory

Domain

172 – 368

197

CheB-type methylesterase

Sites

Active site

192

By similarity

Active site

219

By similarity

Active site

312

By similarity

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Experimental info

Sequence conflict

G → A in BAA33550. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O87125 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: FE4801DC220C613B
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FASTA

368

39,004

        10         20         30         40         50         60 
MAVKVLVVDD SGFFRRRVSE ILSADGQIQV VGTGTNGREA IEQALALRPD VITMDYEMPL 

        70         80         90        100        110        120 
MDGITAVRNI MQRCPTPVLM FSSLTHEGAR VTLDALDAGA VDYLPKNFED ISRNPDKVRQ 

       130        140        150        160        170        180 
LLCEKVLTIA RSNRRSISLP PLPSATSSSH APASSSSVGA SARVGAGASP APASTSAAPK 

       190        200        210        220        230        240 
RKAYRLVAIG TSTGGPVALQ RVLTQLPANF PAPLVLIQHM PAAFTKAFAE RLDKLCRINV 

       250        260        270        280        290        300 
KEAEDGDILR PGLALLAPGG KQMMVDGRGT VRILPGDERL NYKPCVDVTF GSAAKAYNDK 

       310        320        330        340        350        360 
VLAVVLTGMG ADGREGARLL KQGGSQVWAQ DEASCVIYGM PMAVVKANLA DAVYGLDDIG 


RHLVEACQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa." Kato J., Nakamura T., Kuroda A., Ohtake H. Biosci. Biotechnol. Biochem. 63:155-161(1999) [PubMed: 10052136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response." Ferrandez A., Hawkins A.C., Summerfield D.T., Harwood C.S. J. Bacteriol. 184:4374-4383(2002) [PubMed: 12142407] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012767 Genomic DNA. Translation: BAA33550.1. AE004091 Genomic DNA. Translation: AAG04848.1.
PIR	G83463. T46615.
RefSeq	NP_250150.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O87125.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882084.
GenomeReviews	Gene locus PA1459 in contig AE004091_GR.
KEGG	pae:PA1459.
NMPDR	fig\|208964.1.peg.1460.
PATRIC	19837248. VBIPseAer58763_1510.
Organism-specific databases
PseudoCAP	PA1459.
Phylogenomic databases
HOGENOM	HBG705324.
OMA	KMAAELN.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	PAER208964:PA1459-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_PSEAE

Accession

Primary (citable) accession number: O87125

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents