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Protein

tRNA pseudouridine synthase A

Gene

truA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691NucleophileUniRule annotation
Binding sitei127 – 1271SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BRENDAi5.4.99.12. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase AUniRule annotation (EC:5.4.99.12UniRule annotation)
Alternative name(s):
tRNA pseudouridine(38-40) synthaseUniRule annotation
tRNA pseudouridylate synthase IUniRule annotation
tRNA-uridine isomerase IUniRule annotation
Gene namesi
Name:truAUniRule annotation
Synonyms:hisT
Ordered Locus Names:PA3114
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3114.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285tRNA pseudouridine synthase APRO_0000057434Add
BLAST

Proteomic databases

PaxDbiO87016.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA3114.

Structurei

3D structure databases

ProteinModelPortaliO87016.
SMRiO87016. Positions 17-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA pseudouridine synthase TruA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DI7. Bacteria.
COG0101. LUCA.
HOGENOMiHOG000248672.
InParanoidiO87016.
KOiK06173.
OMAiPWRNVHH.
PhylomeDBiO87016.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

O87016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDVVPPAAA ESAAAGVSRI ALGIEYKGSR YRGWQRQEAG VPSVQEALER
60 70 80 90 100
ALSKVAAEPV GVICAGRTDA AVHASGQVVH FDTAVERPLK AWVMGTNANL
110 120 130 140 150
PADISVTWAR VMPAHFHARF SAMARRYRYA IYNDPIRPAH LAEEVTWNHR
160 170 180 190 200
PLDIGRMREA AQVLVGTHDF TSFRAVQCQA KSPVKTMHHV RLLEHGRLIV
210 220 230 240 250
LDIRGNAFLH HMVRNIAGVL MTIGAGERPI EWAKEVLEAR DRRAGGVTAH
260 270 280
PYGLYLVRVE YPGEFELPER YLGPHFLSGL PDIVG
Length:285
Mass (Da):31,483
Last modified:November 1, 1998 - v1
Checksum:i53F062F8DCE4093C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93274 Genomic DNA. Translation: AAC23941.1.
AE004091 Genomic DNA. Translation: AAG06502.1.
PIRiE83257.
RefSeqiNP_251804.1. NC_002516.2.
WP_003091390.1. NZ_ASJY01000509.1.

Genome annotation databases

EnsemblBacteriaiAAG06502; AAG06502; PA3114.
GeneIDi879910.
KEGGipae:PA3114.
PATRICi19840801. VBIPseAer58763_3266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93274 Genomic DNA. Translation: AAC23941.1.
AE004091 Genomic DNA. Translation: AAG06502.1.
PIRiE83257.
RefSeqiNP_251804.1. NC_002516.2.
WP_003091390.1. NZ_ASJY01000509.1.

3D structure databases

ProteinModelPortaliO87016.
SMRiO87016. Positions 17-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3114.

Proteomic databases

PaxDbiO87016.

Protocols and materials databases

DNASUi879910.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06502; AAG06502; PA3114.
GeneIDi879910.
KEGGipae:PA3114.
PATRICi19840801. VBIPseAer58763_3266.

Organism-specific databases

PseudoCAPiPA3114.

Phylogenomic databases

eggNOGiENOG4105DI7. Bacteria.
COG0101. LUCA.
HOGENOMiHOG000248672.
InParanoidiO87016.
KOiK06173.
OMAiPWRNVHH.
PhylomeDBiO87016.

Enzyme and pathway databases

BRENDAi5.4.99.12. 5087.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRUA_PSEAE
AccessioniPrimary (citable) accession number: O87016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.