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O86963 (GLPO_ENTCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 28, 2011. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-glycerophosphate oxidase
EC=1.1.3.21
Alternative name(s):
Glycerol-3-phosphate oxidase
Gene names
Name:glpO
OrganismEnterococcus casseliflavus (Enterococcus flavescens)
Taxonomic identifier37734 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2.

Cofactor

FAD.

Pathway

Membrane lipid metabolism; glycerophospholipid metabolism.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Mass spectrometry

Molecular mass is 67082 Da from positions 2 - 609. Determined by ESI. Ref.1

Ontologies

Keywords
   Biological processGlycerol metabolism
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentglycerol-3-phosphate dehydrogenase complex

Inferred from electronic annotation. Source: InterPro

   Molecular functionglycerol-3-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: InterPro

glycerol-3-phosphate oxidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 609608Alpha-glycerophosphate oxidase
PRO_0000126107

Regions

Nucleotide binding21 – 4929FAD Potential

Sequences

Sequence LengthMass (Da)Tools
O86963 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 38A17281C16990F6

FASTA60967,216
        10         20         30         40         50         60 
MTFSQKDRKE TIQETAKTTY DVLIIGGGIT GAGVAVQTAA AGMKTVLLEM QDFAEGTSSR 

        70         80         90        100        110        120 
STKLVHGGIR YLKTFDVEVV ADTVRERAIV QQIAPHIPKP DPMLLPIYDE PGATFSLFSV 

       130        140        150        160        170        180 
KVAMDLYDRL ANVTGSKYEN YLLTKEEVLA REPQLQAENL VGGGVYLDFR NNDARLVIEN 

       190        200        210        220        230        240 
IKRAQADGAA MISKAKVVGI LHDEQGIING VEVEDQLTNE RFEVHAKVVI NTTGPWSDIV 

       250        260        270        280        290        300 
RQLDKNDELP PQMRPTKGVH LVVDREKLKV PQPTYFDTGK NDGRMVFVVP RENKTYFGTT 

       310        320        330        340        350        360 
DTDYTGDFAH PTVTQEDVDY LLTIVNERFP HAQITLDDIE ASWAGLRPLI TNNGGSDYNG 

       370        380        390        400        410        420 
GGKGKLSDES FEQIVESVKE YLADERQRPV VEKAVKQAQE RVEASKVDPS QVSRGSSLER 

       430        440        450        460        470        480 
SKDGLLTLAG GKITDYRLMA EGAVKRINEL LQESGASFEL VDSTTYPVSG GELDAANVEE 

       490        500        510        520        530        540 
ELAKLADQAQ TAGFNEAAAT YLAHLYGSNL PQVLNYKTKF EGLDEKESTA LNYSLHEEMV 

       550        560        570        580        590        600 
LTPVDYLLRR TNHILFMRDT LDDVKAGVVA AMTDFFGWSE EEKAAHVLEL NQVIAESDLT 


ALKGGKKDE

« Hide

References

[1]"The soluble alpha-glycerophosphate oxidase from Enterococcus casseliflavus. Sequence homology with the membrane-associated dehydrogenase and kinetic analysis of the recombinant enzyme."
Parsonage D., Luba J., Mallett T.C., Claiborne A.
J. Biol. Chem. 273:23812-23822(1998) [PubMed: 9726992] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13 AND 295-327, CHARACTERIZATION, MASS SPECTROMETRY.
Strain: ATCC 12755 / DSM 4841 / NCFB 2725.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U57498 Genomic DNA. Translation: AAC34739.1.

3D structure databases

ProteinModelPortalO86963.
SMRO86963. Positions 3-604.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
PROSITEPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPO_ENTCA
AccessionPrimary (citable) accession number: O86963
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: June 28, 2011
This is version 63 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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