Reviewed, UniProtKB/Swiss-Prot O86963 (GLPO_ENTCA)

Names and origin

Protein names

Recommended name:
    Alpha-glycerophosphate oxidase
    EC=1.1.3.21
Alternative name(s):
    Glycerol-3-phosphate oxidase

Gene names

Name:

glpO

Organism

Enterococcus casseliflavus (Enterococcus flavescens)

Taxonomic identifier

37734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + O(2) = glycerone phosphate + H(2)O(2).
Cofactor	FAD.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Mass spectrometry	Molecular weight is 67082 Da from positions 2 - 609. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 609	608	Alpha-glycerophosphate oxidase		PRO_0000126107
Regions
	Nucleotide binding	21 – 49	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	O86963-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 38A17281C16990F6	FASTA	609	67,216
	10 20 30 40 50 60 MTFSQKDRKE TIQETAKTTY DVLIIGGGIT GAGVAVQTAA AGMKTVLLEM QDFAEGTSSR 70 80 90 100 110 120 STKLVHGGIR YLKTFDVEVV ADTVRERAIV QQIAPHIPKP DPMLLPIYDE PGATFSLFSV 130 140 150 160 170 180 KVAMDLYDRL ANVTGSKYEN YLLTKEEVLA REPQLQAENL VGGGVYLDFR NNDARLVIEN 190 200 210 220 230 240 IKRAQADGAA MISKAKVVGI LHDEQGIING VEVEDQLTNE RFEVHAKVVI NTTGPWSDIV 250 260 270 280 290 300 RQLDKNDELP PQMRPTKGVH LVVDREKLKV PQPTYFDTGK NDGRMVFVVP RENKTYFGTT 310 320 330 340 350 360 DTDYTGDFAH PTVTQEDVDY LLTIVNERFP HAQITLDDIE ASWAGLRPLI TNNGGSDYNG 370 380 390 400 410 420 GGKGKLSDES FEQIVESVKE YLADERQRPV VEKAVKQAQE RVEASKVDPS QVSRGSSLER 430 440 450 460 470 480 SKDGLLTLAG GKITDYRLMA EGAVKRINEL LQESGASFEL VDSTTYPVSG GELDAANVEE 490 500 510 520 530 540 ELAKLADQAQ TAGFNEAAAT YLAHLYGSNL PQVLNYKTKF EGLDEKESTA LNYSLHEEMV 550 560 570 580 590 600 LTPVDYLLRR TNHILFMRDT LDDVKAGVVA AMTDFFGWSE EEKAAHVLEL NQVIAESDLT ALKGGKKDE

References

[1]

"The soluble alpha-glycerophosphate oxidase from Enterococcus casseliflavus. Sequence homology with the membrane-associated dehydrogenase and kinetic analysis of the recombinant enzyme." Parsonage D., Luba J., Mallett T.C., Claiborne A. J. Biol. Chem. 273:23812-23822(1998) [PubMed: 9726992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13 AND 295-327, CHARACTERIZATION, MASS SPECTROMETRY. Strain: ATCC 12755 / DSM 4841 / NCFB 2725.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U57498 Genomic DNA. Translation: AAC34739.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000447. FAD-dep_Gly3P_DHase. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPO_ENTCA

Accession

Primary (citable) accession number: O86963

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)