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Reviewed, UniProtKB/Swiss-Prot O86956 (MGTA_THENE)

Last modified June 16, 2009. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    4-alpha-glucanotransferase
    EC=2.4.1.25
Alternative name(s):
    Amylomaltase
    Disproportionating enzyme
      Short name=D-enzyme
Gene names
Name: mgtA
OrganismThermotoga neapolitana
Taxonomic identifier2337 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

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Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   LigandCalcium
Metal-binding
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function4-alpha-glucanotransferase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4424424-alpha-glucanotransferase
PRO_0000054337

Sites

Active site1861Nucleophile By similarity
Active site2161Proton donor By similarity
Active site2781 By similarity
Metal binding131Calcium By similarity
Metal binding151Calcium By similarity
Metal binding171Calcium By similarity
Metal binding191Calcium; via carbonyl oxygen By similarity
Metal binding211Calcium By similarity

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Sequences

Sequence LengthMass (Da)Tools
O86956-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: AD42748834568CC7

FASTA44251,935
        10         20         30         40         50         60 
MIGYQIYVRS FRDGNFDGVG DFKGLKGAIS YLKELGVDFV WLMPVFSSIS FHGYDVVDFY 

        70         80         90        100        110        120 
SFKAEYGDEK DFREMIEAFH DNGIKVVLDL PIHHTGFLHT WFQKALKGDP HYRDYYVWAS 

       130        140        150        160        170        180 
EKTDLDERRE WDNERIWHPL EDGRFYRGLF GPLSPDLNYD NPQVFEEMKK VVYHLLEMGV 

       190        200        210        220        230        240 
DGFRFDAAKH MRDTLEQNVR FWRYFLSDIE GIFLAEIWAE SKVVDEHGRI FGYMLNFDTS 

       250        260        270        280        290        300 
HCIKEAVWKE NFKVLIESIE RALVGKDYLP VNFTSNHDMS RLASFEGGLS EEKVKLSLSI 

       310        320        330        340        350        360 
LFTLPGVPLI FYGDELGMKG IYRKPNTEVV LDPFPWSENI SLEGQTFWKW PAYNSPFSGV 

       370        380        390        400        410        420 
SVEYQKKKRD SILLHIMKWT GFRKENHWLD RANIEFLCKE EKLLHVYRLV DEGRSLKVIH 

       430        440 
NLSNGEMVFE GVRVQPYSTE VI

« Hide

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References

[1]Zverlov V.V., Mashchenko O.V., Liebl W., Velikodvorskaya G.A.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Z2706-MC24.

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Cross-references

Sequence databases

AJ009831 Genomic DNA. Translation: CAA08864.1.

3D structure databases

HSSPHSSP built from PDB template 1LWJ based on UniProtKB P80099.
ModBaseSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Enzyme and pathway databases

BRENDA2.4.1.25. 1590.

Family and domain databases

InterProIPR015261. 4-alpha-glucanotransf_C.
IPR006046. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat.
IPR006589. Glyco_hydro_13_sub_cat.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF09178. DUF1945. 1 hit.
[Graphical view]
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMGTA_THENE
AccessionPrimary (citable) accession number: O86956
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents