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O86956

- MGTA_THENE

UniProt

O86956 - MGTA_THENE

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Protein
4-alpha-glucanotransferase
Gene
mgtA
Organism
Thermotoga neapolitana
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes the 1,4-alpha-glycoside bonds in oligomeric and polymeric 1,4-alpha-glucans and transfers oligosaccharides (maltotriose being the shortest one) to acceptor maltodextrins.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.1 Publication

Cofactori

Binds 1 calcium ion per subunit By similarity.

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 85 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Calcium By similarity
Metal bindingi15 – 151Calcium By similarity
Metal bindingi17 – 171Calcium By similarity
Metal bindingi19 – 191Calcium; via carbonyl oxygen By similarity
Metal bindingi21 – 211Calcium By similarity
Active sitei186 – 1861Nucleophile By similarity
Active sitei216 – 2161Proton donor By similarity
Sitei278 – 2781Transition state stabilizer By similarity

GO - Molecular functioni

  1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
4-alpha-glucanotransferase (EC:2.4.1.25)
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name:
D-enzyme
Maltodextrin glycosyltransferase
Gene namesi
Name:mgtA
OrganismiThermotoga neapolitana
Taxonomic identifieri2337 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4424424-alpha-glucanotransferase
PRO_0000054337Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO86956.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015261. 4-alpha-glucanotransf_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09178. DUF1945. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

O86956-1 [UniParc]FASTAAdd to Basket

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MIGYQIYVRS FRDGNFDGVG DFKGLKGAIS YLKELGVDFV WLMPVFSSIS    50
FHGYDVVDFY SFKAEYGDEK DFREMIEAFH DNGIKVVLDL PIHHTGFLHT 100
WFQKALKGDP HYRDYYVWAS EKTDLDERRE WDNERIWHPL EDGRFYRGLF 150
GPLSPDLNYD NPQVFEEMKK VVYHLLEMGV DGFRFDAAKH MRDTLEQNVR 200
FWRYFLSDIE GIFLAEIWAE SKVVDEHGRI FGYMLNFDTS HCIKEAVWKE 250
NFKVLIESIE RALVGKDYLP VNFTSNHDMS RLASFEGGLS EEKVKLSLSI 300
LFTLPGVPLI FYGDELGMKG IYRKPNTEVV LDPFPWSENI SLEGQTFWKW 350
PAYNSPFSGV SVEYQKKKRD SILLHIMKWT GFRKENHWLD RANIEFLCKE 400
EKLLHVYRLV DEGRSLKVIH NLSNGEMVFE GVRVQPYSTE VI 442
Length:442
Mass (Da):51,935
Last modified:November 1, 1998 - v1
Checksum:iAD42748834568CC7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ009831 Genomic DNA. Translation: CAA08864.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ009831 Genomic DNA. Translation: CAA08864.1 .

3D structure databases

ProteinModelPortali O86956.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015261. 4-alpha-glucanotransf_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF09178. DUF1945. 1 hit.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene and properties of thermostable 4-alpha-glucanotransferase of Thermotoga neapolitana."
    Berezina O.V., Zverlov V.V., Lunina N.A., Chekanovskaya L.A., Dubinina E.N., Liebl W., Velikodvorskaya G.A.
    Mol. Biol. (Mosk.) 33:801-806(1999)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: Z2706-MC24.

Entry informationi

Entry nameiMGTA_THENE
AccessioniPrimary (citable) accession number: O86956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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