Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O86956 (MGTA_THENE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-alpha-glucanotransferase

EC=2.4.1.25
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme
Maltodextrin glycosyltransferase
Gene names
Name:mgtA
OrganismThermotoga neapolitana
Taxonomic identifier2337 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes the 1,4-alpha-glycoside bonds in oligomeric and polymeric 1,4-alpha-glucans and transfers oligosaccharides (maltotriose being the shortest one) to acceptor maltodextrins. Ref.1

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan. Ref.1

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0. Ref.1

Temperature dependence:

Optimum temperature is 85 degrees Celsius.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   LigandCalcium
Metal-binding
   Molecular functionGlycosyltransferase
Transferase
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4-alpha-glucanotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4424424-alpha-glucanotransferase
PRO_0000054337

Sites

Active site1861Nucleophile By similarity
Active site2161Proton donor By similarity
Metal binding131Calcium By similarity
Metal binding151Calcium By similarity
Metal binding171Calcium By similarity
Metal binding191Calcium; via carbonyl oxygen By similarity
Metal binding211Calcium By similarity
Site2781Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
O86956 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: AD42748834568CC7

FASTA44251,935
        10         20         30         40         50         60 
MIGYQIYVRS FRDGNFDGVG DFKGLKGAIS YLKELGVDFV WLMPVFSSIS FHGYDVVDFY 

        70         80         90        100        110        120 
SFKAEYGDEK DFREMIEAFH DNGIKVVLDL PIHHTGFLHT WFQKALKGDP HYRDYYVWAS 

       130        140        150        160        170        180 
EKTDLDERRE WDNERIWHPL EDGRFYRGLF GPLSPDLNYD NPQVFEEMKK VVYHLLEMGV 

       190        200        210        220        230        240 
DGFRFDAAKH MRDTLEQNVR FWRYFLSDIE GIFLAEIWAE SKVVDEHGRI FGYMLNFDTS 

       250        260        270        280        290        300 
HCIKEAVWKE NFKVLIESIE RALVGKDYLP VNFTSNHDMS RLASFEGGLS EEKVKLSLSI 

       310        320        330        340        350        360 
LFTLPGVPLI FYGDELGMKG IYRKPNTEVV LDPFPWSENI SLEGQTFWKW PAYNSPFSGV 

       370        380        390        400        410        420 
SVEYQKKKRD SILLHIMKWT GFRKENHWLD RANIEFLCKE EKLLHVYRLV DEGRSLKVIH 

       430        440 
NLSNGEMVFE GVRVQPYSTE VI 

« Hide

References

[1]"Gene and properties of thermostable 4-alpha-glucanotransferase of Thermotoga neapolitana."
Berezina O.V., Zverlov V.V., Lunina N.A., Chekanovskaya L.A., Dubinina E.N., Liebl W., Velikodvorskaya G.A.
Mol. Biol. (Mosk.) 33:801-806(1999)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: Z2706-MC24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ009831 Genomic DNA. Translation: CAA08864.1.

3D structure databases

ProteinModelPortalO86956.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProIPR015261. 4-alpha-glucanotransf_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF09178. DUF1945. 1 hit.
[Graphical view]
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMGTA_THENE
AccessionPrimary (citable) accession number: O86956
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries