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O86956

- MGTA_THENE

UniProt

O86956 - MGTA_THENE

Protein

4-alpha-glucanotransferase

Gene

mgtA

Organism
Thermotoga neapolitana
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Hydrolyzes the 1,4-alpha-glycoside bonds in oligomeric and polymeric 1,4-alpha-glucans and transfers oligosaccharides (maltotriose being the shortest one) to acceptor maltodextrins.1 Publication

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.1 Publication

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 85 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi13 – 131CalciumBy similarity
    Metal bindingi15 – 151CalciumBy similarity
    Metal bindingi17 – 171CalciumBy similarity
    Metal bindingi19 – 191Calcium; via carbonyl oxygenBy similarity
    Metal bindingi21 – 211CalciumBy similarity
    Active sitei186 – 1861NucleophileBy similarity
    Active sitei216 – 2161Proton donorBy similarity
    Sitei278 – 2781Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-alpha-glucanotransferase (EC:2.4.1.25)
    Alternative name(s):
    Amylomaltase
    Disproportionating enzyme
    Short name:
    D-enzyme
    Maltodextrin glycosyltransferase
    Gene namesi
    Name:mgtA
    OrganismiThermotoga neapolitana
    Taxonomic identifieri2337 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4424424-alpha-glucanotransferasePRO_0000054337Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO86956.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015261. 4-alpha-glucanotransf_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF09178. DUF1945. 1 hit.
    [Graphical view]
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O86956-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIGYQIYVRS FRDGNFDGVG DFKGLKGAIS YLKELGVDFV WLMPVFSSIS    50
    FHGYDVVDFY SFKAEYGDEK DFREMIEAFH DNGIKVVLDL PIHHTGFLHT 100
    WFQKALKGDP HYRDYYVWAS EKTDLDERRE WDNERIWHPL EDGRFYRGLF 150
    GPLSPDLNYD NPQVFEEMKK VVYHLLEMGV DGFRFDAAKH MRDTLEQNVR 200
    FWRYFLSDIE GIFLAEIWAE SKVVDEHGRI FGYMLNFDTS HCIKEAVWKE 250
    NFKVLIESIE RALVGKDYLP VNFTSNHDMS RLASFEGGLS EEKVKLSLSI 300
    LFTLPGVPLI FYGDELGMKG IYRKPNTEVV LDPFPWSENI SLEGQTFWKW 350
    PAYNSPFSGV SVEYQKKKRD SILLHIMKWT GFRKENHWLD RANIEFLCKE 400
    EKLLHVYRLV DEGRSLKVIH NLSNGEMVFE GVRVQPYSTE VI 442
    Length:442
    Mass (Da):51,935
    Last modified:November 1, 1998 - v1
    Checksum:iAD42748834568CC7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ009831 Genomic DNA. Translation: CAA08864.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ009831 Genomic DNA. Translation: CAA08864.1 .

    3D structure databases

    ProteinModelPortali O86956.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015261. 4-alpha-glucanotransf_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF09178. DUF1945. 1 hit.
    [Graphical view ]
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene and properties of thermostable 4-alpha-glucanotransferase of Thermotoga neapolitana."
      Berezina O.V., Zverlov V.V., Lunina N.A., Chekanovskaya L.A., Dubinina E.N., Liebl W., Velikodvorskaya G.A.
      Mol. Biol. (Mosk.) 33:801-806(1999)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: Z2706-MC24.

    Entry informationi

    Entry nameiMGTA_THENE
    AccessioniPrimary (citable) accession number: O86956
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3