Reviewed, UniProtKB/Swiss-Prot O86841 (DAPA1_STRCO)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase 1
      Short name=DHDPS 1
    EC=4.2.1.52

Gene names

Name:	dapA1
Ordered Locus Names:	SCO5744
ORF Names:	SC9A10.08

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Dihydrodipicolinate synthase 1 HAMAP MF_00418		PRO_0000103165
Regions
	Region	57 – 58	2	Pyruvate binding By similarity
Sites
	Active site	170	1	Schiff-base intermediate with substrate By similarity
	Binding site	115	1	Pyruvate By similarity
	Site	142	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	O86841-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 44FC9B2094E69676	FASTA	299	31,175
	10 20 30 40 50 60 MAPTSTPQTP FGRVLTAMVT PFTADGALDL DGAQRLAAHL VDAGNDGLII NGTTGESPTT 70 80 90 100 110 120 SDAEKADLVR AVVEAVGDRA HVVAGVGTNN TQHSIELARA AERVGAHGLL LVTPYYNKPP 130 140 150 160 170 180 QEGLYLHFTA IADAAGLPVM LYDIPGRSGV PINTETLVRL AEHPRIVANK DAKGDLGRAS 190 200 210 220 230 240 WAIARSGLAW YSGDDMLNLP LLAVGAVGFV SVVGHVVTPE LRAMVDAHVA GDVQKALEIH 250 260 270 280 290 QKLLPVFTGM FRTQGVMTTK GALALQGLPA GPLRAPMVGL TPEETEQLKI DLAAGGVQL

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939124 Genomic DNA. Translation: CAA20295.1.
PIR	T35844.
RefSeq	NP_629869.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Genome annotation databases
GeneID	1101183.
GenomeReviews	Gene locus SCO5744 in contig AL645882_GR.
KEGG	sco:SCO5744.
NMPDR	fig|100226.1.peg.5693.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O86841.
OMA	VAPRLMH.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO5744-MON.
BRENDA	4.2.1.52. 1084.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA1_STRCO

Accession

Primary (citable) accession number: O86841

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)