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O86820

- END8B_STRCO

UniProt

O86820 - END8B_STRCO

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Protein

Probable endonuclease 8 2

Gene

nei

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.PROSITE-ProRule annotation

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotationNote: Binds 1 zinc ion per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
Active sitei3 – 31Proton donorPROSITE-ProRule annotation
Active sitei51 – 511Proton donor; for beta-elimination activityPROSITE-ProRule annotation
Binding sitei165 – 1651DNABy similarity
Active sitei250 – 2501Proton donor; for delta-elimination activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri222 – 26039FPG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
  3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 8 2
Alternative name(s):
DNA glycosylase/AP lyase Nei 2 (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase Nei 2 (EC:4.2.99.18)
Endonuclease VIII 2
Gene namesi
Name:nei
Ordered Locus Names:SCO5760
ORF Names:SC7C7.15c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 276275Probable endonuclease 8 2PRO_0000170902Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5760.

Structurei

3D structure databases

ProteinModelPortaliO86820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation
Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri222 – 26039FPG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0266.
HOGENOMiHOG000020880.
InParanoidiO86820.
KOiK05522.
OMAiGPDVLDM.
OrthoDBiEOG6QP131.
PhylomeDBiO86820.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O86820-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEGDTVWQA ARRLHDALAG RVLTRSDFRV PRYATVDLTG RTVLDVTPRG
60 70 80 90 100
KHLLTRVEGG LTVHSHLRMD GSWKVFAPGQ RWSGGPAHQI RVILGTADRT
110 120 130 140 150
AVGYRLPVLD ILRTAEEQRA VGHLGPDLLG PDWDPERALD NLRADPPRAL
160 170 180 190 200
GEALLDQRNL AGIGNVYKSE LCFLLGVTPW LPVGELPADR AARLPTLAKK
210 220 230 240 250
LLEANRDRPV RRTTGLRGQD LFVYGRAPRP CLRCGTSVRV ADQGDGSRER
260 270
PTYWCPTCQA GPAPRPGGRT GVRPRR
Length:276
Mass (Da):30,408
Last modified:January 23, 2007 - v3
Checksum:i01106CD65EF94DE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA19861.1.
PIRiT35693.
RefSeqiNP_629885.1. NC_003888.3.
WP_011030437.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA19861; CAA19861; CAA19861.
GeneIDi1101202.
KEGGisco:SCO5760.
PATRICi23741404. VBIStrCoe124346_5849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA19861.1 .
PIRi T35693.
RefSeqi NP_629885.1. NC_003888.3.
WP_011030437.1. NC_003888.3.

3D structure databases

ProteinModelPortali O86820.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA19861 ; CAA19861 ; CAA19861 .
GeneIDi 1101202.
KEGGi sco:SCO5760.
PATRICi 23741404. VBIStrCoe124346_5849.

Phylogenomic databases

eggNOGi COG0266.
HOGENOMi HOG000020880.
InParanoidi O86820.
KOi K05522.
OMAi GPDVLDM.
OrthoDBi EOG6QP131.
PhylomeDBi O86820.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiEND8B_STRCO
AccessioniPrimary (citable) accession number: O86820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3