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O86820 (END8B_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable endonuclease 8 2
Alternative name(s):
DNA glycosylase/AP lyase Nei 2
EC=3.2.2.-
DNA-(apurinic or apyrimidinic site) lyase Nei 2
EC=4.2.99.18
Endonuclease VIII 2
Gene names
Name:nei
Ordered Locus Names:SCO5760
ORF Names:SC7C7.15c
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.

Catalytic activity

Removes damaged bases from DNA, leaving an abasic site.

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 276275Probable endonuclease 8 2
PRO_0000170902

Regions

Zinc finger222 – 26039FPG-type

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site511Proton donor; for beta-elimination activity By similarity
Active site2501Proton donor; for delta-elimination activity By similarity
Binding site1651DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
O86820 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 01106CD65EF94DE9

FASTA27630,408
        10         20         30         40         50         60 
MPEGDTVWQA ARRLHDALAG RVLTRSDFRV PRYATVDLTG RTVLDVTPRG KHLLTRVEGG 

        70         80         90        100        110        120 
LTVHSHLRMD GSWKVFAPGQ RWSGGPAHQI RVILGTADRT AVGYRLPVLD ILRTAEEQRA 

       130        140        150        160        170        180 
VGHLGPDLLG PDWDPERALD NLRADPPRAL GEALLDQRNL AGIGNVYKSE LCFLLGVTPW 

       190        200        210        220        230        240 
LPVGELPADR AARLPTLAKK LLEANRDRPV RRTTGLRGQD LFVYGRAPRP CLRCGTSVRV 

       250        260        270 
ADQGDGSRER PTYWCPTCQA GPAPRPGGRT GVRPRR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL939125 Genomic DNA. Translation: CAA19861.1.
PIRT35693.
RefSeqNP_629885.1. NC_003888.3.

3D structure databases

ProteinModelPortalO86820.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO5760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA19861; CAA19861; CAA19861.
GeneID1101202.
KEGGsco:SCO5760.
PATRIC23741404. VBIStrCoe124346_5849.

Phylogenomic databases

eggNOGCOG0266.
HOGENOMHOG000020880.
KOK05522.
OMAGPDVLDM.
OrthoDBEOG6QP131.
PhylomeDBO86820.

Family and domain databases

InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND8B_STRCO
AccessionPrimary (citable) accession number: O86820
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families