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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi117 – 1171IronUniRule annotation
Metal bindingi121 – 1211IronUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation
Binding sitei187 – 1871Substrate; via amide nitrogenUniRule annotation
Binding sitei191 – 1911SubstrateUniRule annotation
Binding sitei283 – 2831SubstrateUniRule annotation
Metal bindingi311 – 3111IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:SCO4752
ORF Names:SC6G4.30
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096973Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO4752.

Structurei

3D structure databases

ProteinModelPortaliO86793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1445Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
InParanoidiO86793.
KOiK01409.
OMAiDHVHEVS.
OrthoDBiPOG091H010B.
PhylomeDBiO86793.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O86793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSRDEPLV LGIETSCDET GVGVVRGTTL LADAVASSVD EHARFGGVVP
60 70 80 90 100
EVASRAHLEA MVPTIDRALK EAGVSARDLD GIAVTAGPGL AGALLVGVSA
110 120 130 140 150
AKAYAYALGK PLYGVNHLAS HICVDQLEHG ALPEPTMALL VSGGHSSLLL
160 170 180 190 200
STDITSDVRP LGATIDDAAG EAFDKIARVL NLGFPGGPVI DRYAREGDPN
210 220 230 240 250
AIAFPRGLTG PRDAAYDFSF SGLKTAVARW IEAKRAAGEE VPVRDVSASF
260 270 280 290 300
QEAVVDVLTR KAVRACKDEG VDHLMIGGGV AANSRLRALA QERCEAAGIR
310 320 330 340 350
LRVPRPKLCT DNGAMVAALG AEMVARNRAA SDWDLSADSS LPVTEPHVPG
360 370
QGHPHGHPHG HDHVHEVSKE NLYS
Length:374
Mass (Da):38,986
Last modified:November 1, 1998 - v1
Checksum:i1259EE6D4ED0DFE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939121 Genomic DNA. Translation: CAA20408.1.
PIRiT35581.
RefSeqiNP_628910.1. NC_003888.3.
WP_011029841.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA20408; CAA20408; CAA20408.
GeneIDi1100193.
KEGGisco:SCO4752.
PATRICi23739328. VBIStrCoe124346_4824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939121 Genomic DNA. Translation: CAA20408.1.
PIRiT35581.
RefSeqiNP_628910.1. NC_003888.3.
WP_011029841.1. NC_003888.3.

3D structure databases

ProteinModelPortaliO86793.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO4752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA20408; CAA20408; CAA20408.
GeneIDi1100193.
KEGGisco:SCO4752.
PATRICi23739328. VBIStrCoe124346_4824.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
InParanoidiO86793.
KOiK01409.
OMAiDHVHEVS.
OrthoDBiPOG091H010B.
PhylomeDBiO86793.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSAD_STRCO
AccessioniPrimary (citable) accession number: O86793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.