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O86528

- SYE_STRCO

UniProt

O86528 - SYE_STRCO

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi112 – 1121ZincUniRule annotation
    Metal bindingi114 – 1141ZincUniRule annotation
    Metal bindingi139 – 1391ZincUniRule annotation
    Metal bindingi141 – 1411ZincUniRule annotation
    Binding sitei263 – 2631ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:SCO5547
    ORF Names:SC1C2.28
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Glutamate--tRNA ligasePRO_0000119663Add
    BLAST

    Proteomic databases

    PRIDEiO86528.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi100226.SCO5547.

    Structurei

    3D structure databases

    ProteinModelPortaliO86528.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi15 – 2511"HIGH" regionAdd
    BLAST
    Motifi260 – 2645"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiPEGMLNY.
    OrthoDBiEOG6DRPF7.
    PhylomeDBiO86528.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O86528-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASASGSPVR VRFCPSPTGN PHVGLVRTAL FNWAFARHHQ GTLVFRIEDT    50
    DAARDSEESY DQLLDSMRWL GFDWDEGPEV GGPHAPYRQS QRMDIYQDVA 100
    QKLLDAGHAY RCYCSQEELD TRREAARAAG KPSGYDGHCR ELTDAQVEEY 150
    TSQGREPIVR FRMPDEAITF TDLVRGEITY LPENVPDYGI VRANGAPLYT 200
    LVNPVDDALM EITHVLRGED LLSSTPRQIA LYKALIELGV AKEIPAFGHL 250
    PYVMGEGNKK LSKRDPQSSL NLYRERGFLP EGLLNYLSLL GWSLSADQDI 300
    FTIEEMVAAF DVSDVQPNPA RFDLKKCEAI NGDHIRLLEV KDFTERCRPW 350
    LKAPVAPWAP EDFDEAKWQA IAPHAQTRLK VLSEITDNVD FLFLPEPVFD 400
    EASWTKAMKE GSDALLTTAR EKLDAADWTS PEALKEAVLA AGEAHGLKLG 450
    KAQAPVRVAV TGRTVGLPLF ESLEVLGKEK ALARIDAALA RLAA 494
    Length:494
    Mass (Da):54,917
    Last modified:November 1, 1998 - v1
    Checksum:iAC3F4EF4D58D0FAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939124 Genomic DNA. Translation: CAA19995.1.
    PIRiT29077.
    RefSeqiNP_629681.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAA19995; CAA19995; CAA19995.
    GeneIDi1100987.
    KEGGisco:SCO5547.
    PATRICi23740958. VBIStrCoe124346_5635.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939124 Genomic DNA. Translation: CAA19995.1 .
    PIRi T29077.
    RefSeqi NP_629681.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali O86528.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO5547.

    Proteomic databases

    PRIDEi O86528.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA19995 ; CAA19995 ; CAA19995 .
    GeneIDi 1100987.
    KEGGi sco:SCO5547.
    PATRICi 23740958. VBIStrCoe124346_5635.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi PEGMLNY.
    OrthoDBi EOG6DRPF7.
    PhylomeDBi O86528.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiSYE_STRCO
    AccessioniPrimary (citable) accession number: O86528
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3