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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi112ZincUniRule annotation1
Metal bindingi114ZincUniRule annotation1
Metal bindingi139ZincUniRule annotation1
Metal bindingi141ZincUniRule annotation1
Binding sitei263ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:SCO5547
ORF Names:SC1C2.28
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196631 – 494Glutamate--tRNA ligaseAdd BLAST494

Proteomic databases

PRIDEiO86528

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO5547

Structurei

3D structure databases

ProteinModelPortaliO86528
SMRiO86528
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi15 – 25"HIGH" regionUniRule annotationAdd BLAST11
Motifi260 – 264"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
InParanoidiO86528
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W
PhylomeDBiO86528

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

O86528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASGSPVR VRFCPSPTGN PHVGLVRTAL FNWAFARHHQ GTLVFRIEDT
60 70 80 90 100
DAARDSEESY DQLLDSMRWL GFDWDEGPEV GGPHAPYRQS QRMDIYQDVA
110 120 130 140 150
QKLLDAGHAY RCYCSQEELD TRREAARAAG KPSGYDGHCR ELTDAQVEEY
160 170 180 190 200
TSQGREPIVR FRMPDEAITF TDLVRGEITY LPENVPDYGI VRANGAPLYT
210 220 230 240 250
LVNPVDDALM EITHVLRGED LLSSTPRQIA LYKALIELGV AKEIPAFGHL
260 270 280 290 300
PYVMGEGNKK LSKRDPQSSL NLYRERGFLP EGLLNYLSLL GWSLSADQDI
310 320 330 340 350
FTIEEMVAAF DVSDVQPNPA RFDLKKCEAI NGDHIRLLEV KDFTERCRPW
360 370 380 390 400
LKAPVAPWAP EDFDEAKWQA IAPHAQTRLK VLSEITDNVD FLFLPEPVFD
410 420 430 440 450
EASWTKAMKE GSDALLTTAR EKLDAADWTS PEALKEAVLA AGEAHGLKLG
460 470 480 490
KAQAPVRVAV TGRTVGLPLF ESLEVLGKEK ALARIDAALA RLAA
Length:494
Mass (Da):54,917
Last modified:November 1, 1998 - v1
Checksum:iAC3F4EF4D58D0FAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA Translation: CAA19995.1
PIRiT29077
RefSeqiNP_629681.1, NC_003888.3
WP_003973448.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAA19995; CAA19995; CAA19995
GeneIDi1100987
KEGGisco:SCO5547
PATRICifig|100226.15.peg.5635

Similar proteinsi

Entry informationi

Entry nameiSYE_STRCO
AccessioniPrimary (citable) accession number: O86528
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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