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O86528

- SYE_STRCO

UniProt

O86528 - SYE_STRCO

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Protein
Glutamate--tRNA ligase
Gene
gltX, SCO5547, SC1C2.28
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi112 – 1121Zinc By similarity
Metal bindingi114 – 1141Zinc By similarity
Metal bindingi139 – 1391Zinc By similarity
Metal bindingi141 – 1411Zinc By similarity
Binding sitei263 – 2631ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:SCO5547
ORF Names:SC1C2.28
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Glutamate--tRNA ligaseUniRule annotation
PRO_0000119663Add
BLAST

Proteomic databases

PRIDEiO86528.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO5547.

Structurei

3D structure databases

ProteinModelPortaliO86528.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2511"HIGH" regionUniRule annotation
Add
BLAST
Motifi260 – 2645"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiPEGMLNY.
OrthoDBiEOG6DRPF7.
PhylomeDBiO86528.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

O86528-1 [UniParc]FASTAAdd to Basket

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MASASGSPVR VRFCPSPTGN PHVGLVRTAL FNWAFARHHQ GTLVFRIEDT    50
DAARDSEESY DQLLDSMRWL GFDWDEGPEV GGPHAPYRQS QRMDIYQDVA 100
QKLLDAGHAY RCYCSQEELD TRREAARAAG KPSGYDGHCR ELTDAQVEEY 150
TSQGREPIVR FRMPDEAITF TDLVRGEITY LPENVPDYGI VRANGAPLYT 200
LVNPVDDALM EITHVLRGED LLSSTPRQIA LYKALIELGV AKEIPAFGHL 250
PYVMGEGNKK LSKRDPQSSL NLYRERGFLP EGLLNYLSLL GWSLSADQDI 300
FTIEEMVAAF DVSDVQPNPA RFDLKKCEAI NGDHIRLLEV KDFTERCRPW 350
LKAPVAPWAP EDFDEAKWQA IAPHAQTRLK VLSEITDNVD FLFLPEPVFD 400
EASWTKAMKE GSDALLTTAR EKLDAADWTS PEALKEAVLA AGEAHGLKLG 450
KAQAPVRVAV TGRTVGLPLF ESLEVLGKEK ALARIDAALA RLAA 494
Length:494
Mass (Da):54,917
Last modified:November 1, 1998 - v1
Checksum:iAC3F4EF4D58D0FAB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939124 Genomic DNA. Translation: CAA19995.1.
PIRiT29077.
RefSeqiNP_629681.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA19995; CAA19995; CAA19995.
GeneIDi1100987.
KEGGisco:SCO5547.
PATRICi23740958. VBIStrCoe124346_5635.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939124 Genomic DNA. Translation: CAA19995.1 .
PIRi T29077.
RefSeqi NP_629681.1. NC_003888.3.

3D structure databases

ProteinModelPortali O86528.
ModBasei Search...

Protein-protein interaction databases

STRINGi 100226.SCO5547.

Proteomic databases

PRIDEi O86528.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA19995 ; CAA19995 ; CAA19995 .
GeneIDi 1100987.
KEGGi sco:SCO5547.
PATRICi 23740958. VBIStrCoe124346_5635.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi PEGMLNY.
OrthoDBi EOG6DRPF7.
PhylomeDBi O86528.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiSYE_STRCO
AccessioniPrimary (citable) accession number: O86528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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