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Protein
Submitted name:

Putative secreted lyase

Gene

SCO5534

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei152 – 1521N-acetyl-D-glucosamineCombined sources
Binding sitei264 – 2641N-acetyl-D-glucosamineCombined sources
Binding sitei318 – 3181N-acetyl-D-glucosamineCombined sources
Binding sitei322 – 3221N-acetyl-D-glucosamineCombined sources
Metal bindingi664 – 6641Magnesium; via carbonyl oxygenCombined sources
Metal bindingi681 – 6811MagnesiumCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseImported

Keywords - Ligandi

MagnesiumCombined sources, Metal-bindingCombined sources

Protein family/group databases

CAZyiPL8. Polysaccharide Lyase Family 8.

Names & Taxonomyi

Protein namesi
Submitted name:
Putative secreted lyaseImported
Gene namesi
Ordered Locus Names:SCO5534Imported
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)Imported
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence analysisAdd
BLAST
Chaini33 – 776744Sequence analysisPRO_5004160258Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5534.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WCOX-ray1.94A33-776[»]
2WDAX-ray2.30A33-776[»]
2X03X-ray2.30A/B33-776[»]
ProteinModelPortaliO86516.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO86516.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 367323Lyase_8_NInterPro annotationAdd
BLAST
Domaini409 – 651243Lyase_8InterPro annotationAdd
BLAST
Domaini666 – 72863Lyase_8_CInterPro annotationAdd
BLAST

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiENOG410XTFC. LUCA.
HOGENOMiHOG000243554.
InParanoidiO86516.
KOiK01727.
OMAiYLWEHAK.
OrthoDBiEOG6GBM67.
PhylomeDBiO86516.

Family and domain databases

Gene3Di1.50.10.100. 1 hit.
2.60.220.10. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR008929. Chondroitin_lyas.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011071. Lyase_8-like_C.
IPR012970. Lyase_8_alpha_N.
IPR004103. Lyase_8_C.
IPR003159. Lyase_8_central_dom.
IPR012329. Lyase_8_N.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF02278. Lyase_8. 1 hit.
PF02884. Lyase_8_C. 1 hit.
PF08124. Lyase_8_N. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.
SSF49863. SSF49863. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O86516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPQRPTAPS RRAVLLAAAA AAALTTVPRA AAATADPYDA LRRRWLGITL
60 70 80 90 100
GTGYDPAAEP YASRLAETGE RAREHRATMA PTPTSLWPGH PFDPPAGITF
110 120 130 140 150
AYGRLWTMTE AYVQEGTGAT GDPALLADIL RGLDHLSATV YHPATTRYGN
160 170 180 190 200
WWEWQIGSPR LLMDITAALY DHLGADRVAA ACAAVDHFVP DAMLGAYTGT
210 220 230 240 250
STGANRVDLC RSVALRGVLG RAPAKIALAR DALSPVFPYV TKGDGLYADG
260 270 280 290 300
SFVQHTWVAY SGTYGQVMLD GLGRLFTLLA GSEWEVTDPG RQLVLDSVEH
310 320 330 340 350
AYAPLIHDGL VMDTVNGRAI SRGYLKSDDL HVMRSDHFHG QQLIAAMAVL
360 370 380 390 400
AGGASNAERE RWHARIKGWI ERDTVTPVLT APQFPVADLT RLHAIADAPG
410 420 430 440 450
EAAPEPVGHH LFAAMDRAVH RRPAFTAGLA MASDRIAHYE CGNGENPRGW
460 470 480 490 500
HTGAGMLTWW ANGTRADQYT DWFWPTVDWY RLPGTTVSTK RLADRAGGEW
510 520 530 540 550
GAPKPDVRWV GGATDGEYAA VGQHLKGLGS TLEARKSWFF LDDAVVCLGA
560 570 580 590 600
GITCADGVPV ETVVDNRNLG EGGTQALVRG RHWAHLEGHG GWIVPGGALR
610 620 630 640 650
TLREDRTGAW SDINTTSTTE RRTRRWQTLW LDHGTDPAGA DYVYTVMPGA
660 670 680 690 700
SRAALARRAA DRHWLTVLAN DDRRQAVSVP SLGLTAANFW QAGTAGPLTT
710 720 730 740 750
TAGASVLVRR RGRTATLRVS EPPRTGEALE IVWDHPVGAV LRADETVEIL
760 770
ATGRRLHLRV TPGVVCTTHE CEVTLS
Length:776
Mass (Da):83,774
Last modified:November 1, 1998 - v1
Checksum:iDE861687595B29E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA19982.1.
PIRiT29064.
RefSeqiNP_629668.1. NC_003888.3.
WP_011030298.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA19982; CAA19982; CAA19982.
GeneIDi1100974.
KEGGisco:SCO5534.
PATRICi23740930. VBIStrCoe124346_5621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA19982.1.
PIRiT29064.
RefSeqiNP_629668.1. NC_003888.3.
WP_011030298.1. NC_003888.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WCOX-ray1.94A33-776[»]
2WDAX-ray2.30A33-776[»]
2X03X-ray2.30A/B33-776[»]
ProteinModelPortaliO86516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO5534.

Protein family/group databases

CAZyiPL8. Polysaccharide Lyase Family 8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA19982; CAA19982; CAA19982.
GeneIDi1100974.
KEGGisco:SCO5534.
PATRICi23740930. VBIStrCoe124346_5621.

Phylogenomic databases

eggNOGiENOG410XTFC. LUCA.
HOGENOMiHOG000243554.
InParanoidiO86516.
KOiK01727.
OMAiYLWEHAK.
OrthoDBiEOG6GBM67.
PhylomeDBiO86516.

Miscellaneous databases

EvolutionaryTraceiO86516.

Family and domain databases

Gene3Di1.50.10.100. 1 hit.
2.60.220.10. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR008929. Chondroitin_lyas.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011071. Lyase_8-like_C.
IPR012970. Lyase_8_alpha_N.
IPR004103. Lyase_8_C.
IPR003159. Lyase_8_central_dom.
IPR012329. Lyase_8_N.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF02278. Lyase_8. 1 hit.
PF02884. Lyase_8_C. 1 hit.
PF08124. Lyase_8_N. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.
SSF49863. SSF49863. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145Imported.
  2. "Crystal structures of a family 8 polysaccharide lyase reveal open and highly occluded substrate-binding cleft conformations."
    Elmabrouk Z.H., Vincent F., Zhang M., Smith N.L., Turkenburg J.P., Charnock S.J., Black G.W., Taylor E.J.
    Proteins 79:965-974(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 33-776 IN COMPLEX WITH MAGNESIUM AND N-ACETYL-D-GLUCOSAMINE.

Entry informationi

Entry nameiO86516_STRCO
AccessioniPrimary (citable) accession number: O86516
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: March 16, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.