Putative secreted lyase - Streptomyces coelicolor

Preferred order of sections

Names and origin

Protein names

Submitted name:
Putative secreted lyase EMBL CAA19982.1

Gene names

Ordered Locus Names:

SCO5534

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Lyase EMBL CAA19982.1
Technical term	3D-structure PDB 2WCO PDB 2WDA PDB 2X03 Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro carbon-oxygen lyase activity, acting on polysaccharides Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Binding site

152

1

N-acetyl-D-glucosamine PDB 2WCO

Binding site

264

1

N-acetyl-D-glucosamine PDB 2WCO

Binding site

318

1

N-acetyl-D-glucosamine PDB 2WCO

Binding site

322

1

N-acetyl-D-glucosamine PDB 2WCO

Sequences

Sequence

Length

Mass (Da)

Tools

O86516 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: DE861687595B29E1
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FASTA

776

83,774

        10         20         30         40         50         60 
MTPQRPTAPS RRAVLLAAAA AAALTTVPRA AAATADPYDA LRRRWLGITL GTGYDPAAEP 

        70         80         90        100        110        120 
YASRLAETGE RAREHRATMA PTPTSLWPGH PFDPPAGITF AYGRLWTMTE AYVQEGTGAT 

       130        140        150        160        170        180 
GDPALLADIL RGLDHLSATV YHPATTRYGN WWEWQIGSPR LLMDITAALY DHLGADRVAA 

       190        200        210        220        230        240 
ACAAVDHFVP DAMLGAYTGT STGANRVDLC RSVALRGVLG RAPAKIALAR DALSPVFPYV 

       250        260        270        280        290        300 
TKGDGLYADG SFVQHTWVAY SGTYGQVMLD GLGRLFTLLA GSEWEVTDPG RQLVLDSVEH 

       310        320        330        340        350        360 
AYAPLIHDGL VMDTVNGRAI SRGYLKSDDL HVMRSDHFHG QQLIAAMAVL AGGASNAERE 

       370        380        390        400        410        420 
RWHARIKGWI ERDTVTPVLT APQFPVADLT RLHAIADAPG EAAPEPVGHH LFAAMDRAVH 

       430        440        450        460        470        480 
RRPAFTAGLA MASDRIAHYE CGNGENPRGW HTGAGMLTWW ANGTRADQYT DWFWPTVDWY 

       490        500        510        520        530        540 
RLPGTTVSTK RLADRAGGEW GAPKPDVRWV GGATDGEYAA VGQHLKGLGS TLEARKSWFF 

       550        560        570        580        590        600 
LDDAVVCLGA GITCADGVPV ETVVDNRNLG EGGTQALVRG RHWAHLEGHG GWIVPGGALR 

       610        620        630        640        650        660 
TLREDRTGAW SDINTTSTTE RRTRRWQTLW LDHGTDPAGA DYVYTVMPGA SRAALARRAA 

       670        680        690        700        710        720 
DRHWLTVLAN DDRRQAVSVP SLGLTAANFW QAGTAGPLTT TAGASVLVRR RGRTATLRVS 

       730        740        750        760        770 
EPPRTGEALE IVWDHPVGAV LRADETVEIL ATGRRLHLRV TPGVVCTTHE CEVTLS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"Crystal structures of a family 8 polysaccharide lyase reveal open and highly occluded substrate-binding cleft conformations." Elmabrouk Z.H., Vincent F., Zhang M., Smith N.L., Turkenburg J.P., Charnock S.J., Black G.W., Taylor E.J. Proteins 79:965-974(2011) [PubMed: 21287626] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 33-776 IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL939124 Genomic DNA. Translation: CAA19982.1.

PIR

T29064.

RefSeq

NP_629668.1. NC_003888.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WCO	X-ray	1.94	A	33-776	[»]
2WDA	X-ray	2.30	A	33-776	[»]
2X03	X-ray	2.30	A/B	33-776	[»]

ProteinModelPortal

O86516.

ModBase

Search...

Protein family/group databases

CAZy

PL8. Polysaccharide Lyase Family 8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1100974.

GenomeReviews

Gene locus SCO5534 in contig AL645882_GR.

KEGG

sco:SCO5534.

NMPDR

fig|100226.1.peg.5486.

PATRIC

23740930. VBIStrCoe124346_5621.

Phylogenomic databases

HOGENOM

HBG699337.

OMA

TKTYRNI.

ProtClustDB

CLSK570151.

Family and domain databases

InterPro

IPR008929. Chondroitin_lyas.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011071. Lyase_8-like_C.
IPR012970. Lyase_8_alpha_N.
IPR004103. Lyase_8_C.
IPR003159. Lyase_8_central_dom.
IPR012329. Lyase_8_N.
IPR006311. TAT_signal.
[Graphical view]

Gene3D

G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:2.60.220.10. Lyase_8_C. 1 hit.
G3DSA:1.50.10.100. Lyase_8_N. 1 hit.

KO

K01727.

Pfam

PF02278. Lyase_8. 1 hit.
PF02884. Lyase_8_C. 1 hit.
PF08124. Lyase_8_N. 1 hit.
[Graphical view]

SUPFAM

SSF48230. Chondroitin_lyas. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49863. Lyase_like_C. 1 hit.

PROSITE

PS51318. TAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O86516_STRCO

Accession

Primary (citable) accession number: O86516

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)