ID   CALB_PSEUH              Reviewed;         481 AA.
AC   O86447;
DT   11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 39.
DE   RecName: Full=Coniferyl aldehyde dehydrogenase;
DE            Short=CALDH;
DE            EC=1.2.1.68;
GN   Name=calB;
OS   Pseudomonas sp. (strain HR199 / DSM 7063).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=86003;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20, AND
RP   CHARACTERIZATION.
RX   MEDLINE=98389649; PubMed=9721273;
RA   Achterholt S., Priefert H., Steinbuechel A.;
RT   "Purification and characterization of the coniferyl aldehyde
RT   dehydrogenase from Pseudomonas sp. strain HR199 and molecular
RT   characterization of the gene.";
RL   J. Bacteriol. 180:4387-4391(1998).
CC   -!- FUNCTION: Catalyzes the NAD+-dependent oxidation of coniferyl
CC       aldehyde to ferulic acid and which is induced during growth with
CC       eugenol as the carbon source.
CC   -!- CATALYTIC ACTIVITY: Coniferyl aldehyde + H(2)O + NAD(P)(+) =
CC       ferulate + NAD(P)H.
CC   -!- SUBUNIT: Homodimer.
CC   -!- MISCELLANEOUS: The enzyme activity with NADP(+) is 4.3% of that
CC       with NAD(+).
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; AJ006231; CAA06926.1; -; Genomic_DNA.
DR   HSSP; P11883; 1AD3.
DR   GO; GO:0004030; F:aldehyde dehydrogenase [NAD(P)+] activity; IEA:InterPro.
DR   GO; GO:0050269; F:coniferyl-aldehyde dehydrogenase activity; IEA:EC.
DR   GO; GO:0006081; P:cellular aldehyde metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR016160; Ald_DH_CS.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH.
DR   InterPro; IPR012394; Aldehyde_DH_NAD(P).
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   PANTHER; PTHR11699:SF15; ALDH; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PIRSF; PIRSF036492; ALDH; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; FALSE_NEG.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    481       Coniferyl aldehyde dehydrogenase.
FT                                /FTId=PRO_0000056585.
FT   ACT_SITE    221    221       By similarity.
FT   ACT_SITE    255    255       By similarity.
SQ   SEQUENCE   481 AA;  51987 MW;  A1AC6CC1CB1D55B1 CRC64;
     MSILGLNGAP VGAEQLGSAL DRMKKAHLEQ GPANLELRLS RLDRAIAMLL ENREAIADAV
     SADFGNRSRE QTLLCDIAGS VASLKDSREH VAKWMEPEHH KAMFPGAEAR VEFQPLGVVG
     VISPWNFPIV LAFGPLAGIF AAGNRAMLKP SELTPRTSAL LAELIARYFD ETELTTVLGD
     AEVGALFSAQ PFDHLIFTGG TAVAKHIMRA AADNLVPVTL ELGGKSPVIV SRSADMADVA
     QRVLTVKTFN AGQICLAPDY VLLPEESLDS FVAEATRFVA AMYPSLLDNP DYTSIINARN
     FDRLHRYLTD AQAKGGRVIE INPAAEELGD SGIRKIAPTL IVNVSDEMLV LNEEIFGPLL
     PIKTYRDFDS AIDYVNSKQR PLASYFFGED AVEREQVLKR TVSGAVVVND VMSHVMMDTL
     PFGGVGHSGM GAYHGIYGFR TFSHAKPVLV QSPVGESNLA MRAPYGEAIH GLLSVLLSTE
     C
//