Reviewed, UniProtKB/Swiss-Prot O86447 (CALB_PSEUH)

Names and origin

Protein names

Recommended name:
    Coniferyl aldehyde dehydrogenase
      Short name=CALDH
    EC=1.2.1.68

Gene names

Name:

calB

Organism

Pseudomonas sp. (strain HR199 / DSM 7063)

Taxonomic identifier

86003 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NAD+-dependent oxidation of coniferyl aldehyde to ferulic acid and which is induced during growth with eugenol as the carbon source.
Catalytic activity	Coniferyl aldehyde + H(2)O + NAD(P)(+) = ferulate + NAD(P)H.
Subunit structure	Homodimer.
Miscellaneous	The enzyme activity with NADP(+) is 4.3% of that with NAD(+).
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: InterPro coniferyl-aldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 481	480	Coniferyl aldehyde dehydrogenase		PRO_0000056585
Sites
	Active site	221	1	By similarity
	Active site	255	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O86447-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A1AC6CC1CB1D55B1	FASTA	481	51,987
	10 20 30 40 50 60 MSILGLNGAP VGAEQLGSAL DRMKKAHLEQ GPANLELRLS RLDRAIAMLL ENREAIADAV 70 80 90 100 110 120 SADFGNRSRE QTLLCDIAGS VASLKDSREH VAKWMEPEHH KAMFPGAEAR VEFQPLGVVG 130 140 150 160 170 180 VISPWNFPIV LAFGPLAGIF AAGNRAMLKP SELTPRTSAL LAELIARYFD ETELTTVLGD 190 200 210 220 230 240 AEVGALFSAQ PFDHLIFTGG TAVAKHIMRA AADNLVPVTL ELGGKSPVIV SRSADMADVA 250 260 270 280 290 300 QRVLTVKTFN AGQICLAPDY VLLPEESLDS FVAEATRFVA AMYPSLLDNP DYTSIINARN 310 320 330 340 350 360 FDRLHRYLTD AQAKGGRVIE INPAAEELGD SGIRKIAPTL IVNVSDEMLV LNEEIFGPLL 370 380 390 400 410 420 PIKTYRDFDS AIDYVNSKQR PLASYFFGED AVEREQVLKR TVSGAVVVND VMSHVMMDTL 430 440 450 460 470 480 PFGGVGHSGM GAYHGIYGFR TFSHAKPVLV QSPVGESNLA MRAPYGEAIH GLLSVLLSTE C

References

[1]

"Purification and characterization of the coniferyl aldehyde dehydrogenase from Pseudomonas sp. strain HR199 and molecular characterization of the gene." Achterholt S., Priefert H., Steinbuechel A. J. Bacteriol. 180:4387-4391(1998) [PubMed: 9721273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ006231 Genomic DNA. Translation: CAA06926.1.
3D structure databases
HSSP	HSSP built from PDB template 1AD3 based on UniProtKB P11883.
ModBase	Search...
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR012394. Ald_DHase_NAD(P). IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036492. ALDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALB_PSEUH

Accession

Primary (citable) accession number: O86447

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 38 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)