ID   UDG_PSEAE               Reviewed;         453 AA.
AC   O86422; Q9I292;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   04-MAY-2001, sequence version 2.
DT   16-JUN-2009, entry version 64.
DE   RecName: Full=UDP-glucose 6-dehydrogenase;
DE            Short=UDP-Glc dehydrogenase;
DE            Short=UDP-GlcDH;
DE            Short=UDPGDH;
DE            EC=1.1.1.22;
GN   Name=udg; Synonyms=ugd; OrderedLocusNames=PA2022;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RA   Peng H.L., Chang J.T., Lee J.H., Chang H.Y.;
RT   "Organization of UDP glucose dehydrogenase and UDP glucose
RT   pyrophosphorylase gene cluster in Pseudomonas aeruginosa PAO1.";
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY: UDP-glucose + 2 NAD(+) + H(2)O = UDP-
CC       glucuronate + 2 NADH.
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; UDP-glucuronic acid
CC       biosynthesis; UDP-glucuronic acid from UDP-glucose: step 1/1.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC       family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA09327.1; Type=Frameshift; Positions=336, 372, 429;
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DR   EMBL; AJ010734; CAA09327.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AE004091; AAG05410.1; -; Genomic_DNA.
DR   PIR; H83393; H83393.
DR   RefSeq; NP_250712.1; -.
DR   HSSP; P11759; 1MV8.
DR   GeneID; 879866; -.
DR   GenomeReviews; AE004091_GR; PA2022.
DR   KEGG; pae:PA2022; -.
DR   NMPDR; fig|208964.1.peg.2022; -.
DR   PseudoCAP; PA2022; -.
DR   HOGENOM; O86422; -.
DR   OMA; O86422; VEARNAR.
DR   BioCyc; PAER208964:PA2022-MON; -.
DR   BRENDA; 1.1.1.22; 354.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0003979; F:UDP-glucose 6-dehydrogenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR017476; Nucleotide_sugar_DH.
DR   InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR   InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR   InterPro; IPR014028; UDP-Glc/GDP-Man_DH_dimer-bd.
DR   InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Gene3D; G3DSA:3.40.50.1870; UDP-Glc/GDP-Man_DH_C; 1.
DR   PANTHER; PTHR11374; UDPG_MGDP_DH_Creg; 1.
DR   Pfam; PF00984; UDPG_MGDP_dh; 1.
DR   Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR   Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR   TIGRFAMs; TIGR03026; NDP-sugDHase; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase.
FT   CHAIN         1    453       UDP-glucose 6-dehydrogenase.
FT                                /FTId=PRO_0000074049.
FT   NP_BIND       2     19       NAD (Potential).
FT   ACT_SITE    266    266       By similarity.
FT   CONFLICT     81     81       F -> L (in Ref. 1; CAA09327).
FT   CONFLICT    453    453       A -> R (in Ref. 1; CAA09327).
SQ   SEQUENCE   453 AA;  49690 MW;  A8E7954D0D11557D CRC64;
     MRLCVIGAGY VGLVTAACFA EMGNQVRCVE RDRERVARLR RGEMPIYEPG LESILRDQLD
     AARLTFTASL AEGLADAEVV FIAVGTPCGE DGSADLSHVL AVAEQLGAQL RQACIVVNKS
     TVPVGTAERV EEIIRLGLAR RRKRFRVAVA SNPEFLKEGS AVDDFRRPDR VIIGSAETQA
     GETLRQLYAP FLRNHERVLL MGRREAEFSK YAANAFLATK ISFMNEMAGL CALTGVDIED
     VRRGMGSDKR IGTHFIYAGC GYGGSCFPKD VRALIRSAEQ QGYDSQILRA VEARNARQKE
     LLFETLGELF QGRWQGRTVA LWGLAFKPGT DDLREAPSLV LLEALLRHGV RVRAHDPVAN
     AGVAARYPEA VACARLTLHD SPYAAVEGAD ALVLVTEWKQ FRQPDFQKIR GSMRTPLLVD
     GRNLYAPARM AELGFIYQGI GRPRAGHCKA SAA
//