O86422 (UDG_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 6-dehydrogenase
Short name=UDP-Glc dehydrogenase
Short name=UDP-GlcDH
Short name=UDPGDH
EC=1.1.1.22

Gene names

Name:	udg
Synonyms:	ugd
Ordered Locus Names:	PA2022

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
Sequence caution	The sequence CAA09327.1 differs from that shown. Reason: Frameshift at positions 336, 372 and 429.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-glucuronate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	UDP-glucose 6-dehydrogenase		PRO_0000074049
Regions
	Nucleotide binding	2 – 19	18	NAD Potential
Sites
	Active site	266	1	By similarity
Experimental info
	Sequence conflict	81	1	F → L in CAA09327. Ref.1
	Sequence conflict	453	1	A → R in CAA09327. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O86422 [UniParc]. Last modified May 4, 2001. Version 2. Checksum: A8E7954D0D11557D	FASTA	453	49,690
	10 20 30 40 50 60 MRLCVIGAGY VGLVTAACFA EMGNQVRCVE RDRERVARLR RGEMPIYEPG LESILRDQLD 70 80 90 100 110 120 AARLTFTASL AEGLADAEVV FIAVGTPCGE DGSADLSHVL AVAEQLGAQL RQACIVVNKS 130 140 150 160 170 180 TVPVGTAERV EEIIRLGLAR RRKRFRVAVA SNPEFLKEGS AVDDFRRPDR VIIGSAETQA 190 200 210 220 230 240 GETLRQLYAP FLRNHERVLL MGRREAEFSK YAANAFLATK ISFMNEMAGL CALTGVDIED 250 260 270 280 290 300 VRRGMGSDKR IGTHFIYAGC GYGGSCFPKD VRALIRSAEQ QGYDSQILRA VEARNARQKE 310 320 330 340 350 360 LLFETLGELF QGRWQGRTVA LWGLAFKPGT DDLREAPSLV LLEALLRHGV RVRAHDPVAN 370 380 390 400 410 420 AGVAARYPEA VACARLTLHD SPYAAVEGAD ALVLVTEWKQ FRQPDFQKIR GSMRTPLLVD 430 440 450 GRNLYAPARM AELGFIYQGI GRPRAGHCKA SAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of UDP glucose dehydrogenase and UDP glucose pyrophosphorylase gene cluster in Pseudomonas aeruginosa PAO1." Peng H.L., Chang J.T., Lee J.H., Chang H.Y. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ010734 Genomic DNA. Translation: CAA09327.1. Frameshift. AE004091 Genomic DNA. Translation: AAG05410.1.
PIR	H83393.
RefSeq	NP_250712.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O86422.
SMR	O86422. Positions 1-443.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA2022.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879866.
KEGG	pae:PA2022.
PATRIC	19838453. VBIPseAer58763_2107.
Organism-specific databases
PseudoCAP	PA2022.
Phylogenomic databases
eggNOG	COG1004.
HOGENOM	HOG000153773.
KO	K00012.
OMA	VRCVERD.
ProtClustDB	CLSK866958.
Enzyme and pathway databases
UniPathway	UPA00030. UPA00038; UER00491.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMART	SM00984. UDPG_MGDP_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UDG_PSEAE

Accession

Primary (citable) accession number: O86422
Secondary accession number(s): Q9I292

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 4, 2001
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program