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Protein
Submitted name:

Cephalosporin acylase

Gene

ca1

Organism
Pseudomonas sp. 130
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BRENDAi3.5.1.93. 5085.

Names & Taxonomyi

Protein namesi
Submitted name:
Cephalosporin acylaseImported
Gene namesi
Name:ca1Imported
OrganismiPseudomonas sp. 130Imported
Taxonomic identifieri81841 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 720691Sequence analysisPRO_5005360157Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GHDX-ray2.40A28-198[»]
B199-720[»]
4E55X-ray2.30A/B31-719[»]
4E56X-ray2.20A/B31-719[»]
4E57X-ray2.00A/B31-719[»]
ProteinModelPortaliO86089.
SMRiO86089. Positions 35-720.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO86089.

Family & Domainsi

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di1.10.439.10. 1 hit.
3.60.20.10. 2 hits.
InterProiIPR029055. Ntn_hydrolases_N.
IPR002692. Pen/cephal_acylase.
IPR023343. Penicillin_amidase_dom1.
[Graphical view]
PfamiPF01804. Penicil_amidase. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequencei

Sequence statusi: Complete.

O86089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVLHRAAS ALVMATVIGL APAVAFALAE PTSTPQAPIA AYKPRSNEIL
60 70 80 90 100
WDGYGVPHIY GVDAPSAFYG YGWAQARSHG DNILRLYGEA RGKGAEYWGP
110 120 130 140 150
DYEQTTVWLL TNGVPERAQQ WYAQQSPDFR ANLDAFAAGI NAYAQQNPDD
160 170 180 190 200
ISPDVRQVLP VSGADVVAHA HRLMNFLYVA SPGRTLGEGD PPDLADQGSN
210 220 230 240 250
SWAVAPGKTA NGNALLLQNP HLSWTTDYFT YYEAHLVTPD FEIYGATQIG
260 270 280 290 300
LPVIRFAFNQ RMGITNTVNG MVGATNYRLT LQDGGYLYDG QVRPFERRQA
310 320 330 340 350
SYRLRQADGT TVDKPLEIRS SVHGPVFERA DGTAVAVRVA GLDRPGMLEQ
360 370 380 390 400
YFDMITADSF DDYEAALARM QVPTFNIVYA DREGTINYSF NGVAPKRAEG
410 420 430 440 450
DIAFWQGLVP GDSSRYLWTE THPLDDLPRV TNPPGGFVQN SNDPPWTPTW
460 470 480 490 500
PVTYTPKDFP SYLAPQTPHS LRAQQSVRLM SENDDLTLER FMALQLSHRA
510 520 530 540 550
VMADRTLPDL IPAALIDPDP EVQAAARLLA AWDREFTSDS RAALLFEEWA
560 570 580 590 600
RLFAGQNFAG QAGFATPWSL DKPVSTPYGV RDPKAAVDQL RTAIANTKRK
610 620 630 640 650
YGAIDRPFGD ASRMILNDVN VPGAAGYGNL GSFRVFTWSD PDENGVRTPV
660 670 680 690 700
HGETWVAMIE FSTPVRAYGL MSYGNSRQPG TTHYSDQIER VSRADFRELL
710 720
LRREQVEAAV QERTPFNFKP
Length:720
Mass (Da):79,672
Last modified:March 1, 2001 - v2
Checksum:iC2EF964F5081D8CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085353 Genomic DNA. Translation: AAC34685.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085353 Genomic DNA. Translation: AAC34685.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GHDX-ray2.40A28-198[»]
B199-720[»]
4E55X-ray2.30A/B31-719[»]
4E56X-ray2.20A/B31-719[»]
4E57X-ray2.00A/B31-719[»]
ProteinModelPortaliO86089.
SMRiO86089. Positions 35-720.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.5.1.93. 5085.

Miscellaneous databases

EvolutionaryTraceiO86089.

Family and domain databases

Gene3Di1.10.439.10. 1 hit.
3.60.20.10. 2 hits.
InterProiIPR029055. Ntn_hydrolases_N.
IPR002692. Pen/cephal_acylase.
IPR023343. Penicillin_amidase_dom1.
[Graphical view]
PfamiPF01804. Penicil_amidase. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130."
    Li Y., Chen J., Jiang W., Mao X., Zhao G., Wang E.
    Eur. J. Biochem. 262:713-719(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 130Imported.
  2. Li Y., Zhang Q., Yang Y., Zhao G., Wang E.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 130Imported.
  3. "Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130."
    Huang X., Zeng R., Ding X., Mao X., Ding Y., Rao Z., Xie Y., Jiang W., Zhao G.
    J. Biol. Chem. 277:10256-10264(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 28-198 AND 199-720.
  4. "Spacer shortened Cephalosporin acylase."
    Yin J., Zhang Z., Wu G., Huang X.
    Submitted (MAR-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 31-719.
  5. "spacer shortened Cephalosporin acylase."
    Yin J., Zhang Z., Wu G., Huang X.
    Submitted (MAR-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 31-719.

Entry informationi

Entry nameiO86089_9PROT
AccessioniPrimary (citable) accession number: O86089
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.