Reviewed, UniProtKB/Swiss-Prot O86062 (NUDC_PSEAE)

Names and origin

Protein names

Recommended name:
    NADH pyrophosphatase
    EC=3.6.1.22

Gene names

Name:	nudC
Ordered Locus Names:	PA1823

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	NAD+ + H2O = AMP + NMN. HAMAP MF_00297
Cofactor	Divalent ions. Magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudC subfamily.

Ontologies

Keywords
Ligand	Magnesium Manganese NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	NAD+ diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	NADH pyrophosphatase HAMAP MF_00297		PRO_0000056973
Regions
	Motif	175 – 196	22	Nudix box HAMAP MF_00297
Experimental info
	Sequence conflict	2	1	A → V in AAC34568. Ref.1
	Sequence conflict	5	1	F → S in AAC34568. Ref.1
	Sequence conflict	9	1	A → V in AAC34568. Ref.1
	Sequence conflict	74	1	E → D in AAC34568. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O86062-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 3CEBD80225D8753A	FASTA	278	31,356
	10 20 30 40 50 60 MAGEFRWQAG RPATAQVGGW VLAHCQQRFL QDDNGLLFPR EWLKRQELPL LAEHGVGHWQ 70 80 90 100 110 120 GEPVYVLELD EPIELPGMAW APLRQFMLHG DFDQFCMLGY ASQIGIWARH NRFCGNCGTR 130 140 150 160 170 180 MQAQDHERVM QCPQCGLHQY PRLSPSMIVL VTRGDEVLLA RSPRFVPGVY STLAGFVEAG 190 200 210 220 230 240 ESVEQCVVRE VREEVGVEVA NLEYIGSQNW PFPHSLMLGF HAEYVSGEIV PQEDEIEDAQ 250 260 270 WFSLDALPPL PAQRSIARHL IDLYLARRSG AAEPVLPG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"fimL, a new gene involved in pilin biogenesis and function in Pseudomonas aeruginosa." Comolli J., Kang P.J., Engel J.N. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29260 / PA103.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF083252 Genomic DNA. Translation: AAC34568.1. AE004091 Genomic DNA. Translation: AAG05212.1.
PIR	A83419.
RefSeq	NP_250514.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880282.
GenomeReviews	Gene locus PA1823 in contig AE004091_GR.
KEGG	pae:PA1823.
NMPDR	fig|208964.1.peg.1824.
Organism-specific databases
PseudoCAP	PA1823.
CMR	Search...
Phylogenomic databases
HOGENOM	O86062.
OMA	O86062. SPCIIVA.
Enzyme and pathway databases
BioCyc	PAER208964:PA1823-MON.
BRENDA	3.6.1.22. 354.
Family and domain databases
HAMAP	MF_00297. [Tree]
InterPro	IPR015375. NADH_PPase-like_N. IPR000086. NUDIX_hydrolase_core. IPR015376. Znr_NADH_PPase. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. PF09296. NUDIX-like. 1 hit. PF09297. zf-NADH-PPase. 1 hit. [Graphical view]
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDC_PSEAE

Accession

Primary (citable) accession number: O86062
Secondary accession number(s): Q9I2S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)