ID   BDHA_RHIME              Reviewed;         258 AA.
AC   O86034;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=D-beta-hydroxybutyrate dehydrogenase;
DE            Short=BDH;
DE            EC=1.1.1.30;
DE   AltName: Full=3-hydroxybutyrate dehydrogenase;
DE            Short=3-HBDH;
GN   Name=bdhA; OrderedLocusNames=RB1136; ORFNames=SMb21010;
OS   Rhizobium meliloti (Sinorhizobium meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=382;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SU47 / 1021;
RX   MEDLINE=99121023; PubMed=9922248;
RA   Aneja P., Charles T.C.;
RT   "Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium)
RT   meliloti: isolation and characterization of a gene encoding 3-
RT   hydroxybutyrate dehydrogenase.";
RL   J. Bacteriol. 181:849-857(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   MEDLINE=21396508; PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P.,
RA   Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J.,
RA   Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-
RT   fixing endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
CC   -!- CATALYTIC ACTIVITY: (R)-3-hydroxybutanoate + NAD(+) = acetoacetate
CC       + NADH.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; AF080548; AAD12733.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49536.1; -; Genomic_DNA.
DR   PIR; H95983; H95983.
DR   RefSeq; NP_437676.1; -.
DR   HSSP; Q10855; 1NFF.
DR   GeneID; 1237467; -.
DR   GenomeReviews; AL591985_GR; RB1136.
DR   KEGG; sme:SM_b21010; -.
DR   NMPDR; fig|266834.1.peg.5771; -.
DR   HOGENOM; O86034; -.
DR   OMA; O86034; QVINEVM.
DR   BioCyc; SMEL266834:SMB21010-MON; -.
DR   BRENDA; 1.1.1.30; 142.
DR   GO; GO:0003858; F:3-hydroxybutyrate dehydrogenase activity; IEA:EC.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR011294; 3-OHbutyrate_DH.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   TIGRFAMs; TIGR01963; PHB_DH; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase; Plasmid.
FT   CHAIN         1    258       D-beta-hydroxybutyrate dehydrogenase.
FT                                /FTId=PRO_0000054528.
FT   NP_BIND       6     30       NAD (By similarity).
FT   ACT_SITE    153    153       Proton acceptor (By similarity).
FT   BINDING     140    140       Substrate (By similarity).
SQ   SEQUENCE   258 AA;  27179 MW;  ACE9D388A4F0D0BA CRC64;
     MTKTAVITGS TSGIGLAIAR TLAKAGANIV LNGFGAPDEI RTVTDEVAGL SSGTVLHHPA
     DMTKPSEIAD MMAMVADRFG GADILVNNAG VQFVEKIEDF PVEQWDRIIA VNLSSSFHTI
     RGAIPPMKKK GWGRIINIAS AHGLVASPFK SAYVAAKHGI MGLTKTVALE VAESGVTVNS
     ICPGYVLTPL VEKQIPDQAR TRGITEEQVI NEVMLKGQPT KKFITVEQVA SLALYLAGDD
     AAQITGTHVS MDGGWTAQ
//