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Protein

D-beta-hydroxybutyrate dehydrogenase

Gene

bdhA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401SubstrateBy similarity
Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 3025NADBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3170-MONOMER.
SMEL266834:GJF6-4569-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-beta-hydroxybutyrate dehydrogenase (EC:1.1.1.30)
Short name:
BDH
Alternative name(s):
3-hydroxybutyrate dehydrogenase
Short name:
3-HBDH
Gene namesi
Name:bdhA
Ordered Locus Names:RB1136
ORF Names:SMb21010
Encoded oniPlasmid pSymB (megaplasmid 2)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymB

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258D-beta-hydroxybutyrate dehydrogenasePRO_0000054528Add
BLAST

Structurei

Secondary structure

1
258
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Helixi13 – 2412Combined sources
Beta strandi28 – 325Combined sources
Helixi37 – 4812Combined sources
Beta strandi55 – 584Combined sources
Helixi65 – 7814Combined sources
Beta strandi79 – 813Combined sources
Beta strandi83 – 875Combined sources
Helixi97 – 993Combined sources
Helixi102 – 11211Combined sources
Helixi114 – 13017Combined sources
Beta strandi133 – 1386Combined sources
Helixi141 – 1433Combined sources
Helixi151 – 17121Combined sources
Helixi172 – 1743Combined sources
Beta strandi176 – 1838Combined sources
Helixi226 – 23712Combined sources
Helixi240 – 2434Combined sources
Beta strandi248 – 2525Combined sources
Helixi255 – 2573Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V2HX-ray3.00A/B1-258[»]
ProteinModelPortaliO86034.
SMRiO86034. Positions 2-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00019.
OMAiHTIRGAI.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011294. 3-OHbutyrate_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01963. PHB_DH. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O86034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTAVITGS TSGIGLAIAR TLAKAGANIV LNGFGAPDEI RTVTDEVAGL
60 70 80 90 100
SSGTVLHHPA DMTKPSEIAD MMAMVADRFG GADILVNNAG VQFVEKIEDF
110 120 130 140 150
PVEQWDRIIA VNLSSSFHTI RGAIPPMKKK GWGRIINIAS AHGLVASPFK
160 170 180 190 200
SAYVAAKHGI MGLTKTVALE VAESGVTVNS ICPGYVLTPL VEKQIPDQAR
210 220 230 240 250
TRGITEEQVI NEVMLKGQPT KKFITVEQVA SLALYLAGDD AAQITGTHVS

MDGGWTAQ
Length:258
Mass (Da):27,179
Last modified:November 1, 1998 - v1
Checksum:iACE9D388A4F0D0BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080548 Genomic DNA. Translation: AAD12733.1.
AL591985 Genomic DNA. Translation: CAC49536.1.
PIRiH95983.
RefSeqiNP_437676.1. NC_003078.1.
WP_010975968.1. NC_003078.1.

Genome annotation databases

EnsemblBacteriaiCAC49536; CAC49536; SM_b21010.
GeneIDi1237467.
KEGGisme:SM_b21010.
PATRICi23638688. VBISinMel96828_6065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080548 Genomic DNA. Translation: AAD12733.1.
AL591985 Genomic DNA. Translation: CAC49536.1.
PIRiH95983.
RefSeqiNP_437676.1. NC_003078.1.
WP_010975968.1. NC_003078.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V2HX-ray3.00A/B1-258[»]
ProteinModelPortaliO86034.
SMRiO86034. Positions 2-258.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC49536; CAC49536; SM_b21010.
GeneIDi1237467.
KEGGisme:SM_b21010.
PATRICi23638688. VBISinMel96828_6065.

Phylogenomic databases

KOiK00019.
OMAiHTIRGAI.
OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3170-MONOMER.
SMEL266834:GJF6-4569-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011294. 3-OHbutyrate_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01963. PHB_DH. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti: isolation and characterization of a gene encoding 3-hydroxybutyrate dehydrogenase."
    Aneja P., Charles T.C.
    J. Bacteriol. 181:849-857(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SU47 / 1021.
  2. "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
    Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
    Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiBDHA_RHIME
AccessioniPrimary (citable) accession number: O86034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.