Reviewed, UniProtKB/Swiss-Prot O86034 (BDHA_RHIME)

Names and origin

Protein names

Recommended name:
    D-beta-hydroxybutyrate dehydrogenase
      Short name=BDH
    EC=1.1.1.30
Alternative name(s):
    3-hydroxybutyrate dehydrogenase
      Short name=3-HBDH

Gene names

Name:	bdhA
Ordered Locus Names:	RB1136
ORF Names:	SMb21010

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxybutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	D-beta-hydroxybutyrate dehydrogenase		PRO_0000054528
Regions
	Nucleotide binding	6 – 30	25	NAD By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O86034-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: ACE9D388A4F0D0BA	FASTA	258	27,179
	10 20 30 40 50 60 MTKTAVITGS TSGIGLAIAR TLAKAGANIV LNGFGAPDEI RTVTDEVAGL SSGTVLHHPA 70 80 90 100 110 120 DMTKPSEIAD MMAMVADRFG GADILVNNAG VQFVEKIEDF PVEQWDRIIA VNLSSSFHTI 130 140 150 160 170 180 RGAIPPMKKK GWGRIINIAS AHGLVASPFK SAYVAAKHGI MGLTKTVALE VAESGVTVNS 190 200 210 220 230 240 ICPGYVLTPL VEKQIPDQAR TRGITEEQVI NEVMLKGQPT KKFITVEQVA SLALYLAGDD 250 AAQITGTHVS MDGGWTAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti: isolation and characterization of a gene encoding 3-hydroxybutyrate dehydrogenase." Aneja P., Charles T.C. J. Bacteriol. 181:849-857(1999) [PubMed: 9922248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SU47 / 1021.
[2]	"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF080548 Genomic DNA. Translation: AAD12733.1. AL591985 Genomic DNA. Translation: CAC49536.1.
PIR	H95983.
RefSeq	NP_437676.1.
3D structure databases
HSSP	HSSP built from PDB template 1NFF based on UniProtKB Q10855.
ModBase	Search...
Genome annotation databases
GeneID	1237467.
GenomeReviews	Gene locus RB1136 in contig AL591985_GR.
KEGG	sme:SMb21010.
NMPDR	fig|266834.1.peg.5771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O86034.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB21010-MON.
Family and domain databases
InterPro	IPR011294. 3-OHbutyrate_DHase. IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01963. PHB_DH. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	O86034.

Entry information

Entry name

BDHA_RHIME

Accession

Primary (citable) accession number: O86034

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)