Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol kinase

Gene

glpK

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateUniRule annotation1
Binding sitei16ATPUniRule annotation1
Binding sitei134Substrate1
Binding sitei265ATPUniRule annotation1
Binding sitei308ATP; via carbonyl oxygenUniRule annotation1
Binding sitei312ATP; via amide nitrogenUniRule annotation1
Binding sitei327ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14ATPUniRule annotation3
Nucleotide bindingi411 – 415ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:RB1135
ORF Names:SMb21009
Encoded oniPlasmid pSymB (megaplasmid 2)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000594831 – 497Glycerol kinaseAdd BLAST497

Structurei

Secondary structure

1497
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi12 – 20Combined sources9
Beta strandi26 – 33Combined sources8
Helixi48 – 64Combined sources17
Turni65 – 67Combined sources3
Helixi70 – 72Combined sources3
Beta strandi73 – 80Combined sources8
Beta strandi85 – 89Combined sources5
Turni90 – 92Combined sources3
Beta strandi95 – 97Combined sources3
Helixi108 – 116Combined sources9
Helixi120 – 127Combined sources8
Helixi136 – 146Combined sources11
Helixi150 – 155Combined sources6
Beta strandi159 – 163Combined sources5
Helixi164 – 172Combined sources9
Turni173 – 175Combined sources3
Beta strandi179 – 181Combined sources3
Helixi182 – 185Combined sources4
Beta strandi188 – 192Combined sources5
Turni193 – 196Combined sources4
Helixi200 – 206Combined sources7
Helixi210 – 212Combined sources3
Beta strandi215 – 217Combined sources3
Beta strandi219 – 221Combined sources3
Helixi228 – 231Combined sources4
Beta strandi236 – 240Combined sources5
Helixi243 – 250Combined sources8
Beta strandi259 – 272Combined sources14
Beta strandi281 – 283Combined sources3
Beta strandi285 – 292Combined sources8
Beta strandi295 – 305Combined sources11
Helixi308 – 316Combined sources9
Helixi328 – 332Combined sources5
Beta strandi341 – 343Combined sources3
Turni351 – 353Combined sources3
Beta strandi361 – 365Combined sources5
Helixi371 – 396Combined sources26
Beta strandi406 – 410Combined sources5
Helixi411 – 414Combined sources4
Helixi416 – 426Combined sources11
Beta strandi430 – 434Combined sources5
Helixi438 – 451Combined sources14
Helixi457 – 462Combined sources6
Beta strandi466 – 470Combined sources5
Helixi476 – 494Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4E1JX-ray2.33A/B/C/D1-497[»]
ProteinModelPortaliO86033.
SMRiO86033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate binding2
Regioni243 – 244Substrate binding2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

KOiK00864.
OMAiPMEIFAT.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O86033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGYILAIDQ GTTSTRAIVF DGNQKIAGVG QKEFKQHFPK SGWVEHDPEE
60 70 80 90 100
IWQTVVSTVK EAIEKSGITA NDIAAIGITN QRETVVVWDR ETGKPIHNAI
110 120 130 140 150
VWQDRRTAAF CDKLKKKGLE KTFVKKTGLL LDPYFSGTKL NWLLSNVKGA
160 170 180 190 200
QVRAAKGELC FGTIDTFLIW RLTGGECFCT DATNASRTLL YNIAENAWDD
210 220 230 240 250
ELTEVLRVPK EMLPEVKDCA ADFGVTDPSL FGAAIPILGV AGDQQAATIG
260 270 280 290 300
QACFKPGMLK STYGTGCFAL LNTGKDMVRS KNRLLTTIAY RLDGETTYAL
310 320 330 340 350
EGSIFVAGAA VQWLRDGLKV IKAAPDTGSL AESADPSQEV YLVPAFTGLG
360 370 380 390 400
APHWDPDARG AIFGMTRNTG PAEFARAALE AVCYQTRDLL EAMHKDWRRN
410 420 430 440 450
GNDTVLRVDG GMVASDWTMQ RLSDLLDAPV DRPVILETTA LGVAWLAGSR
460 470 480 490
AGVWPNQEAF AKSWARDRRF EPHMDEATRK VKLKGWRSAV KRTLIAA
Length:497
Mass (Da):54,419
Last modified:October 18, 2001 - v2
Checksum:i42D3428335ACC2B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591985 Genomic DNA. Translation: CAC49535.1.
AF080548 Genomic DNA. Translation: AAD12735.1.
PIRiG95983.
RefSeqiNP_437675.1. NC_003078.1.
WP_010975967.1. NC_003078.1.

Genome annotation databases

EnsemblBacteriaiCAC49535; CAC49535; SM_b21009.
GeneIDi1237466.
KEGGisme:SM_b21009.
PATRICi23638684. VBISinMel96828_6063.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591985 Genomic DNA. Translation: CAC49535.1.
AF080548 Genomic DNA. Translation: AAD12735.1.
PIRiG95983.
RefSeqiNP_437675.1. NC_003078.1.
WP_010975967.1. NC_003078.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4E1JX-ray2.33A/B/C/D1-497[»]
ProteinModelPortaliO86033.
SMRiO86033.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC49535; CAC49535; SM_b21009.
GeneIDi1237466.
KEGGisme:SM_b21009.
PATRICi23638684. VBISinMel96828_6063.

Phylogenomic databases

KOiK00864.
OMAiPMEIFAT.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK_RHIME
AccessioniPrimary (citable) accession number: O86033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 18, 2001
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.