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O85987 (PDXA_SPHAR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxythreonine-4-phosphate dehydrogenase

EC=1.1.1.262
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase
Gene names
Name:pdxA
Encoded onPlasmid pNL1
OrganismSphingomonas aromaticivorans
Taxonomic identifier48935 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536

Catalytic activity

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536

Cofactor

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00536

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00536.

Miscellaneous

The active site is located at the dimer interface By similarity.

Sequence similarities

Belongs to the PdxA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3303304-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536
PRO_0000188830

Sites

Metal binding1691Divalent metal cation; shared with dimeric partner By similarity
Metal binding2131Divalent metal cation; shared with dimeric partner By similarity
Metal binding2631Divalent metal cation; shared with dimeric partner By similarity
Binding site2711Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O85987 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 55ABBF8B500CE707

FASTA33034,579
        10         20         30         40         50         60 
MSTASERVRP VVGTMIGDPA GIGPEVAVRA LADGAVHTDS IPVLVGSAAA VERALDFTGT 

        70         80         90        100        110        120 
KARLRVMRGF EKPSDDPAII DVIDTGALPD GVLPLGEDTE AAGHATAQWL DELDALARDG 

       130        140        150        160        170        180 
SFAATIMGPI STGSLKLAKK LDRVISPTPG ESYLVLLTGP LRVAHLTDHM SLRQVIDVIS 

       190        200        210        220        230        240 
ADLVATAVGQ LHEAMQSWGI AQPRIAVAGL NPHAMGDEDR LEIAPGIEAA RARGIDVEGP 

       250        260        270        280        290        300 
IAPDSVFRHC IEGRYDMVLA MFHDQGHIAV KTWGFSGNSV IIMGPPYLHM SVAHGTAYDI 

       310        320        330 
VGTGKADAAM MLSAMRTCGR LASGRGFEQA 

« Hide

References

[1]"Complete sequence of a 184 kb catabolic plasmid from Sphingomonas aromaticivorans strain F199."
Romine M.F., Stillwell L.C., Wong K.-K., Thurston S.J., Sisk E.C., Sensen C.W., Gaasterland T., Saffer J.D., Fredrickson J.K.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: F199.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF079317 Genomic DNA. Translation: AAD04003.1.
PIRT31279.
RefSeqNP_049207.1. NC_002033.1.

3D structure databases

ProteinModelPortalO85987.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1238515.

Phylogenomic databases

ProtClustDBCLSK862338.

Enzyme and pathway databases

UniPathwayUPA00244; UER00312.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
HAMAPMF_00536. PdxA.
InterProIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamPF04166. PdxA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDXA_SPHAR
AccessionPrimary (citable) accession number: O85987
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways