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O85987

- PDXA_SPHAR

UniProt

O85987 - PDXA_SPHAR

Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Sphingomonas aromaticivorans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi169 – 1691Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi213 – 2131Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi263 – 2631Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
    Gene namesi
    Name:pdxAUniRule annotation
    Encoded oniPlasmid pNL10 Publication
    OrganismiSphingomonas aromaticivorans
    Taxonomic identifieri48935 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3303304-hydroxythreonine-4-phosphate dehydrogenasePRO_0000188830Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO85987.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O85987-1 [UniParc]FASTAAdd to Basket

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    MSTASERVRP VVGTMIGDPA GIGPEVAVRA LADGAVHTDS IPVLVGSAAA    50
    VERALDFTGT KARLRVMRGF EKPSDDPAII DVIDTGALPD GVLPLGEDTE 100
    AAGHATAQWL DELDALARDG SFAATIMGPI STGSLKLAKK LDRVISPTPG 150
    ESYLVLLTGP LRVAHLTDHM SLRQVIDVIS ADLVATAVGQ LHEAMQSWGI 200
    AQPRIAVAGL NPHAMGDEDR LEIAPGIEAA RARGIDVEGP IAPDSVFRHC 250
    IEGRYDMVLA MFHDQGHIAV KTWGFSGNSV IIMGPPYLHM SVAHGTAYDI 300
    VGTGKADAAM MLSAMRTCGR LASGRGFEQA 330
    Length:330
    Mass (Da):34,579
    Last modified:November 1, 1998 - v1
    Checksum:i55ABBF8B500CE707
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF079317 Genomic DNA. Translation: AAD04003.1.
    PIRiT31279.
    RefSeqiNP_049207.1. NC_002033.1.

    Genome annotation databases

    GeneIDi1238515.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF079317 Genomic DNA. Translation: AAD04003.1 .
    PIRi T31279.
    RefSeqi NP_049207.1. NC_002033.1.

    3D structure databases

    ProteinModelPortali O85987.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1238515.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of a 184 kb catabolic plasmid from Sphingomonas aromaticivorans strain F199."
      Romine M.F., Stillwell L.C., Wong K.-K., Thurston S.J., Sisk E.C., Sensen C.W., Gaasterland T., Saffer J.D., Fredrickson J.K.
      Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: F199.

    Entry informationi

    Entry nameiPDXA_SPHAR
    AccessioniPrimary (citable) accession number: O85987
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3