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O85987

- PDXA_SPHAR

UniProt

O85987 - PDXA_SPHAR

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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Sphingomonas aromaticivorans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi169 – 1691Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi213 – 2131Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi263 – 2631Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. cobalt ion binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro
  5. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Encoded oniPlasmid pNL10 Publication
OrganismiSphingomonas aromaticivorans
Taxonomic identifieri48935 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3303304-hydroxythreonine-4-phosphate dehydrogenasePRO_0000188830Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO85987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85987-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTASERVRP VVGTMIGDPA GIGPEVAVRA LADGAVHTDS IPVLVGSAAA
60 70 80 90 100
VERALDFTGT KARLRVMRGF EKPSDDPAII DVIDTGALPD GVLPLGEDTE
110 120 130 140 150
AAGHATAQWL DELDALARDG SFAATIMGPI STGSLKLAKK LDRVISPTPG
160 170 180 190 200
ESYLVLLTGP LRVAHLTDHM SLRQVIDVIS ADLVATAVGQ LHEAMQSWGI
210 220 230 240 250
AQPRIAVAGL NPHAMGDEDR LEIAPGIEAA RARGIDVEGP IAPDSVFRHC
260 270 280 290 300
IEGRYDMVLA MFHDQGHIAV KTWGFSGNSV IIMGPPYLHM SVAHGTAYDI
310 320 330
VGTGKADAAM MLSAMRTCGR LASGRGFEQA
Length:330
Mass (Da):34,579
Last modified:November 1, 1998 - v1
Checksum:i55ABBF8B500CE707
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF079317 Genomic DNA. Translation: AAD04003.1.
PIRiT31279.
RefSeqiNP_049207.1. NC_002033.1.

Genome annotation databases

GeneIDi1238515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF079317 Genomic DNA. Translation: AAD04003.1 .
PIRi T31279.
RefSeqi NP_049207.1. NC_002033.1.

3D structure databases

ProteinModelPortali O85987.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1238515.

Enzyme and pathway databases

UniPathwayi UPA00244 ; UER00312 .

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
HAMAPi MF_00536. PdxA.
InterProi IPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view ]
Pfami PF04166. PdxA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of a 184 kb catabolic plasmid from Sphingomonas aromaticivorans strain F199."
    Romine M.F., Stillwell L.C., Wong K.-K., Thurston S.J., Sisk E.C., Sensen C.W., Gaasterland T., Saffer J.D., Fredrickson J.K.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: F199.

Entry informationi

Entry nameiPDXA_SPHAR
AccessioniPrimary (citable) accession number: O85987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3