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Protein

Putative 4-hydroxythreonine-4-phosphate dehydrogenase

Gene
N/A
Organism
Novosphingobium aromaticivorans (Sphingomonas aromaticivorans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

Miscellaneous

The active site is located at the dimer interface.By similarity

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.By similarity

Cofactori

Zn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 1 divalent metal cation per subunit. Can use ions such as Zn2+, Mg2+ or Co2+.By similarity

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Putative 4-hydroxythreonine-4-phosphate dehydrogenase
  5. no protein annotated in this organism
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi169Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi213Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi263Divalent metal cation; shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandCobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxythreonine-4-phosphate dehydrogenaseBy similarity (EC:1.1.1.262By similarity)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseBy similarity
Encoded oniPlasmid pNL10 Publication
OrganismiNovosphingobium aromaticivorans (Sphingomonas aromaticivorans)
Taxonomic identifieri48935 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001888301 – 330Putative 4-hydroxythreonine-4-phosphate dehydrogenaseAdd BLAST330

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO85987.
SMRiO85987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR005255. PdxA.
PANTHERiPTHR30004. PTHR30004. 1 hit.
PfamiView protein in Pfam
PF04166. PdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

O85987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTASERVRP VVGTMIGDPA GIGPEVAVRA LADGAVHTDS IPVLVGSAAA
60 70 80 90 100
VERALDFTGT KARLRVMRGF EKPSDDPAII DVIDTGALPD GVLPLGEDTE
110 120 130 140 150
AAGHATAQWL DELDALARDG SFAATIMGPI STGSLKLAKK LDRVISPTPG
160 170 180 190 200
ESYLVLLTGP LRVAHLTDHM SLRQVIDVIS ADLVATAVGQ LHEAMQSWGI
210 220 230 240 250
AQPRIAVAGL NPHAMGDEDR LEIAPGIEAA RARGIDVEGP IAPDSVFRHC
260 270 280 290 300
IEGRYDMVLA MFHDQGHIAV KTWGFSGNSV IIMGPPYLHM SVAHGTAYDI
310 320 330
VGTGKADAAM MLSAMRTCGR LASGRGFEQA
Length:330
Mass (Da):34,579
Last modified:November 1, 1998 - v1
Checksum:i55ABBF8B500CE707
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079317 Genomic DNA. Translation: AAD04003.1.
PIRiT31279.
RefSeqiNP_049207.1. NC_002033.1.
WP_010891025.1. NC_002033.1.

Genome annotation databases

GeneIDi1238515.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPDXAL_NOVAR
AccessioniPrimary (citable) accession number: O85987
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1998
Last modified: May 10, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families