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Protein

Methionyl-tRNA formyltransferase

Gene

fmt

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP (By similarity).By similarity

Catalytic activityi

10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).

GO - Molecular functioni

GO - Biological processi

  • regulation of transmembrane transporter activity Source: PseudoCAP
  • translational initiation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Methionyl-tRNA formyltransferase (EC:2.1.2.9)
Gene namesi
Name:fmt
Ordered Locus Names:PA0018
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0018.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Methionyl-tRNA formyltransferasePRO_0000083017Add
BLAST

Proteomic databases

PaxDbiO85732.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0018.

Structurei

3D structure databases

ProteinModelPortaliO85732.
SMRiO85732. Positions 2-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 1164Tetrahydrofolate (THF) bindingBy similarity

Sequence similaritiesi

Belongs to the Fmt family.Curated

Phylogenomic databases

eggNOGiENOG4105CAE. Bacteria.
COG0223. LUCA.
HOGENOMiHOG000261177.
InParanoidiO85732.
KOiK00604.
OMAiGCINSHA.
OrthoDBiEOG6B09WV.
PhylomeDBiO85732.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
[Graphical view]
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQALRIVFA GTPEFAAEHL KALLDTPHRI VAVYTQPDRP AGRGQKLMPS
60 70 80 90 100
AVKSLALEHG LPVMQPQSLR NAEAQAELAA LRADLMVVVA YGLILPQAVL
110 120 130 140 150
DIPRLGCINS HASLLPRWRG AAPIQRAVEA GDAESGVTVM QMEAGLDTGP
160 170 180 190 200
MLLKVSTPIS AADTGGSLHD RLAALGPKAV IEAIAGLAAG TLHGEIQDDA
210 220 230 240 250
LATYAHKLNK DEARLDWSRP AVELERQVRA FTPWPVCHTS LADAPLKVLG
260 270 280 290 300
ASLGQGSGAP GTILEASRDG LLVACGEGAL RLTRLQLPGG KPLAFADLYN
310
SRREQFAAGQ VLGQ
Length:314
Mass (Da):33,028
Last modified:November 1, 1998 - v1
Checksum:iEA1570CDCD61F269
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073952 Genomic DNA. Translation: AAC26787.1.
AE004091 Genomic DNA. Translation: AAG03408.1.
PIRiG83643.
RefSeqiNP_248708.1. NC_002516.2.
WP_003114642.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03408; AAG03408; PA0018.
GeneIDi879258.
KEGGipae:PA0018.
PATRICi19834230. VBIPseAer58763_0017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073952 Genomic DNA. Translation: AAC26787.1.
AE004091 Genomic DNA. Translation: AAG03408.1.
PIRiG83643.
RefSeqiNP_248708.1. NC_002516.2.
WP_003114642.1. NC_002516.2.

3D structure databases

ProteinModelPortaliO85732.
SMRiO85732. Positions 2-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0018.

Proteomic databases

PaxDbiO85732.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03408; AAG03408; PA0018.
GeneIDi879258.
KEGGipae:PA0018.
PATRICi19834230. VBIPseAer58763_0017.

Organism-specific databases

PseudoCAPiPA0018.

Phylogenomic databases

eggNOGiENOG4105CAE. Bacteria.
COG0223. LUCA.
HOGENOMiHOG000261177.
InParanoidiO85732.
KOiK00604.
OMAiGCINSHA.
OrthoDBiEOG6B09WV.
PhylomeDBiO85732.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
[Graphical view]
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Recognition of the initiator tRNA by the Pseudomonas aeruginosa methionyl-tRNA formyltransferase: importance of the base-base mismatch at the end of the acceptor stem."
    Newton D.T., Niemkiewicz M., Lo R.Y.C., Mangroo D.
    FEMS Microbiol. Lett. 178:289-298(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiFMT_PSEAE
AccessioniPrimary (citable) accession number: O85732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.