O85732 (FMT_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	PA0018

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H2O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: EC methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Methionyl-tRNA formyltransferase HAMAP MF_00182		PRO_0000083017
Regions
	Region	113 – 116	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	O85732 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: EA1570CDCD61F269	FASTA	314	33,028
	10 20 30 40 50 60 MSQALRIVFA GTPEFAAEHL KALLDTPHRI VAVYTQPDRP AGRGQKLMPS AVKSLALEHG 70 80 90 100 110 120 LPVMQPQSLR NAEAQAELAA LRADLMVVVA YGLILPQAVL DIPRLGCINS HASLLPRWRG 130 140 150 160 170 180 AAPIQRAVEA GDAESGVTVM QMEAGLDTGP MLLKVSTPIS AADTGGSLHD RLAALGPKAV 190 200 210 220 230 240 IEAIAGLAAG TLHGEIQDDA LATYAHKLNK DEARLDWSRP AVELERQVRA FTPWPVCHTS 250 260 270 280 290 300 LADAPLKVLG ASLGQGSGAP GTILEASRDG LLVACGEGAL RLTRLQLPGG KPLAFADLYN 310 SRREQFAAGQ VLGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Recognition of the initiator tRNA by the Pseudomonas aeruginosa methionyl-tRNA formyltransferase: importance of the base-base mismatch at the end of the acceptor stem." Newton D.T., Niemkiewicz M., Lo R.Y.C., Mangroo D. FEMS Microbiol. Lett. 178:289-298(1999) [PubMed: 10499278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF073952 Genomic DNA. Translation: AAC26787.1. AE004091 Genomic DNA. Translation: AAG03408.1.
PIR	G83643.
RefSeq	NP_248708.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O85732.
SMR	O85732. Positions 2-312.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879258.
GenomeReviews	Gene locus PA0018 in contig AE004091_GR.
KEGG	pae:PA0018.
NMPDR	fig|208964.1.peg.18.
PATRIC	19834230. VBIPseAer58763_0017.
Organism-specific databases
PseudoCAP	PA0018.
Phylogenomic databases
HOGENOM	HBG571560.
OMA	IMQMDEG.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	PAER208964:PA0018-MONOMER.
Family and domain databases
HAMAP	MF_00182. Formyl_trans. [Tree]
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
Gene3D	G3DSA:3.10.25.10. Formyl_trans_C. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00604.
PANTHER	PTHR11138. Met_tRNA_Form_TA-like. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. Fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_PSEAE

Accession

Primary (citable) accession number: O85732

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program