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Protein
Submitted name:

Cephalosporin hydroxylase CmcI

Gene

cmcI

Organism
Streptomyces clavuligerus
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171Iron-sulfur (4Fe-4S-S-AdoMet); via carbonyl oxygenCombined sources
Metal bindingi18 – 181Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi64 – 641Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi65 – 651Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi87 – 871Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi89 – 891Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi90 – 901Iron-sulfur (4Fe-4S-S-AdoMet); via carbonyl oxygenCombined sources
Metal bindingi91 – 911Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi95 – 951Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Binding sitei95 – 951S-adenosyl-L-homocysteineCombined sources
Metal bindingi116 – 1161Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi117 – 1171Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi121 – 1211Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Binding sitei121 – 1211S-adenosyl-L-homocysteineCombined sources
Metal bindingi138 – 1381Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi139 – 1391Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Metal bindingi161 – 1611Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources
Binding sitei161 – 1611S-adenosyl-L-homocysteineCombined sources
Binding sitei187 – 1871S-adenosyl-L-homocysteineCombined sources

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. methyltransferase activity Source: InterPro

GO - Biological processi

  1. lipid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Ligandi

4Fe-4SCombined sources, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13421.

Names & Taxonomyi

Protein namesi
Submitted name:
Cephalosporin hydroxylase CmcIImported
Gene namesi
Name:cmcIImported
OrganismiStreptomyces clavuligerusImported
Taxonomic identifieri1901 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BM8X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-236[»]
2BM9X-ray2.94A/B/C/D/E/F1-236[»]
2BR3X-ray2.79A/B/C/D/E/F1-236[»]
2BR4X-ray2.59A/B/C/D/E/F1-236[»]
2BR5X-ray2.83A/B/C/D/E/F1-236[»]
ProteinModelPortaliO85726.
SMRiO85726. Positions 2-234.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO85726.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 652S-adenosyl-L-homocysteine bindingCombined sources
Regioni87 – 915S-adenosyl-L-homocysteine bindingCombined sources
Regioni116 – 1172S-adenosyl-L-homocysteine bindingCombined sources
Regioni138 – 1392S-adenosyl-L-homocysteine bindingCombined sources

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007072. Rhamnosyl_O-MeTrfase_CmcI.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04989. CmcI. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

O85726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDYSRQNFL DLNLFRGLGE DPAYHPPVLT DRPRDWPLDR WAEAPRDLGY
60 70 80 90 100
SDFSPYQWRG LRMLKDPDTQ AVYHDMLWEL RPRTIVELGV YNGGSLAWFR
110 120 130 140 150
DLTKIMGIDC QVIGIDRDLS RCQIPASDME NITLHQGDCS DLTTFEHLRE
160 170 180 190 200
MAHPLIFIDD AHANTFNIMK WAVDHLLEEG DYFIIEDMIP YWYRYAPQLL
210 220 230
SEYLGAFRDV LSMDMLYANA SSQLDRGVLR RVAAKQ
Length:236
Mass (Da):27,584
Last modified:November 1, 1998 - v1
Checksum:iFD1EF1B650AF8070
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073896 Genomic DNA. Translation: AAC32491.1.

Genome annotation databases

PATRICi29741601. VBIStrCla15562_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073896 Genomic DNA. Translation: AAC32491.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BM8X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-236[»]
2BM9X-ray2.94A/B/C/D/E/F1-236[»]
2BR3X-ray2.79A/B/C/D/E/F1-236[»]
2BR4X-ray2.59A/B/C/D/E/F1-236[»]
2BR5X-ray2.83A/B/C/D/E/F1-236[»]
ProteinModelPortaliO85726.
SMRiO85726. Positions 2-234.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICi29741601. VBIStrCla15562_0183.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13421.

Miscellaneous databases

EvolutionaryTraceiO85726.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007072. Rhamnosyl_O-MeTrfase_CmcI.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04989. CmcI. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein."
    Alexander D.C., Jensen S.E.
    J. Bacteriol. 180:4068-4079(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NRRL 3585Imported.
  2. "Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus."
    Oster L.M., Lester D.R., Terwisscha van Scheltinga A., Svenda M., van Lun M., Genereux C., Andersson I.
    J. Mol. Biol. 358:546-558(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (4FE-4S-S-ADOMET) AND S-ADENOSYL-L-HOMOCYSTEINE.

Entry informationi

Entry nameiO85726_STRCL
AccessioniPrimary (citable) accession number: O85726
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: April 29, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.