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O85726

- O85726_STRCL

UniProt

O85726 - O85726_STRCL

Protein
Submitted name:

Cephalosporin hydroxylase CmcI

Gene

cmcI

Organism
Streptomyces clavuligerus
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi17 – 171Iron-sulfur(4Fe-4S-S-AdoMet); via carbonyl oxygenImported
    Metal bindingi18 – 181Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi64 – 641Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi65 – 651Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi87 – 871Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi89 – 891Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi90 – 901Iron-sulfur(4Fe-4S-S-AdoMet); via carbonyl oxygenImported
    Metal bindingi91 – 911Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi95 – 951Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Binding sitei95 – 951S-adenosyl-L-homocysteineImported
    Metal bindingi116 – 1161Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi117 – 1171Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi121 – 1211Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Binding sitei121 – 1211S-adenosyl-L-homocysteineImported
    Metal bindingi138 – 1381Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi139 – 1391Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi160 – 1601Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Metal bindingi161 – 1611Iron-sulfur(4Fe-4S-S-AdoMet)Imported
    Binding sitei161 – 1611S-adenosyl-L-homocysteineImported
    Binding sitei187 – 1871S-adenosyl-L-homocysteineImported

    GO - Molecular functioni

    1. methyltransferase activity Source: InterPro

    GO - Biological processi

    1. lipid biosynthetic process Source: InterPro

    Keywords - Ligandi

    Metal-bindingImported

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13421.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Cephalosporin hydroxylase CmcIImported
    Gene namesi
    Name:cmcIImported
    OrganismiStreptomyces clavuligerusImported
    Taxonomic identifieri1901 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BM8X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-236[»]
    2BM9X-ray2.94A/B/C/D/E/F1-236[»]
    2BR3X-ray2.79A/B/C/D/E/F1-236[»]
    2BR4X-ray2.59A/B/C/D/E/F1-236[»]
    2BR5X-ray2.83A/B/C/D/E/F1-236[»]
    ProteinModelPortaliO85726.
    SMRiO85726. Positions 2-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO85726.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 652S-adenosyl-L-homocysteine bindingImported
    Regioni87 – 915S-adenosyl-L-homocysteine bindingImported
    Regioni116 – 1172S-adenosyl-L-homocysteine bindingImported
    Regioni138 – 1392S-adenosyl-L-homocysteine bindingImported

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR007072. Rhamnosyl_O-MeTrfase_CmcI.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF04989. CmcI. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O85726-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDYSRQNFL DLNLFRGLGE DPAYHPPVLT DRPRDWPLDR WAEAPRDLGY    50
    SDFSPYQWRG LRMLKDPDTQ AVYHDMLWEL RPRTIVELGV YNGGSLAWFR 100
    DLTKIMGIDC QVIGIDRDLS RCQIPASDME NITLHQGDCS DLTTFEHLRE 150
    MAHPLIFIDD AHANTFNIMK WAVDHLLEEG DYFIIEDMIP YWYRYAPQLL 200
    SEYLGAFRDV LSMDMLYANA SSQLDRGVLR RVAAKQ 236
    Length:236
    Mass (Da):27,584
    Last modified:November 1, 1998 - v1
    Checksum:iFD1EF1B650AF8070
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF073896 Genomic DNA. Translation: AAC32491.1.

    Genome annotation databases

    PATRICi29741601. VBIStrCla15562_0183.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF073896 Genomic DNA. Translation: AAC32491.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BM8 X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L 1-236 [» ]
    2BM9 X-ray 2.94 A/B/C/D/E/F 1-236 [» ]
    2BR3 X-ray 2.79 A/B/C/D/E/F 1-236 [» ]
    2BR4 X-ray 2.59 A/B/C/D/E/F 1-236 [» ]
    2BR5 X-ray 2.83 A/B/C/D/E/F 1-236 [» ]
    ProteinModelPortali O85726.
    SMRi O85726. Positions 2-234.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    PATRICi 29741601. VBIStrCla15562_0183.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13421.

    Miscellaneous databases

    EvolutionaryTracei O85726.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR007072. Rhamnosyl_O-MeTrfase_CmcI.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF04989. CmcI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein."
      Alexander D.C., Jensen S.E.
      J. Bacteriol. 180:4068-4079(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: NRRL 3585Imported.
    2. "Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus."
      Oster L.M., Lester D.R., Terwisscha van Scheltinga A., Svenda M., van Lun M., Genereux C., Andersson I.
      J. Mol. Biol. 358:546-558(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR(4FE-4S-S-ADOMET) AND S-ADENOSYL-L-HOMOCYSTEINE.

    Entry informationi

    Entry nameiO85726_STRCL
    AccessioniPrimary (citable) accession number: O85726
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1998
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3