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O85726

- O85726_STRCL

UniProt

O85726 - O85726_STRCL

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Protein
Submitted name:

Cephalosporin hydroxylase CmcI

Gene

cmcI

Organism
Streptomyces clavuligerus
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171Iron-sulfur(4Fe-4S-S-AdoMet); via carbonyl oxygenImported
Metal bindingi18 – 181Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi64 – 641Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi65 – 651Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi87 – 871Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi89 – 891Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi90 – 901Iron-sulfur(4Fe-4S-S-AdoMet); via carbonyl oxygenImported
Metal bindingi91 – 911Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi95 – 951Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Binding sitei95 – 951S-adenosyl-L-homocysteineImported
Metal bindingi116 – 1161Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi117 – 1171Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi121 – 1211Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Binding sitei121 – 1211S-adenosyl-L-homocysteineImported
Metal bindingi138 – 1381Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi139 – 1391Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi160 – 1601Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Metal bindingi161 – 1611Iron-sulfur(4Fe-4S-S-AdoMet)Imported
Binding sitei161 – 1611S-adenosyl-L-homocysteineImported
Binding sitei187 – 1871S-adenosyl-L-homocysteineImported

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. methyltransferase activity Source: InterPro

GO - Biological processi

  1. lipid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Metal-bindingImported

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13421.

Names & Taxonomyi

Protein namesi
Submitted name:
Cephalosporin hydroxylase CmcIImported
Gene namesi
Name:cmcIImported
OrganismiStreptomyces clavuligerusImported
Taxonomic identifieri1901 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BM8X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-236[»]
2BM9X-ray2.94A/B/C/D/E/F1-236[»]
2BR3X-ray2.79A/B/C/D/E/F1-236[»]
2BR4X-ray2.59A/B/C/D/E/F1-236[»]
2BR5X-ray2.83A/B/C/D/E/F1-236[»]
ProteinModelPortaliO85726.
SMRiO85726. Positions 2-234.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO85726.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 652S-adenosyl-L-homocysteine bindingImported
Regioni87 – 915S-adenosyl-L-homocysteine bindingImported
Regioni116 – 1172S-adenosyl-L-homocysteine bindingImported
Regioni138 – 1392S-adenosyl-L-homocysteine bindingImported

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007072. Rhamnosyl_O-MeTrfase_CmcI.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF04989. CmcI. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

O85726-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNDYSRQNFL DLNLFRGLGE DPAYHPPVLT DRPRDWPLDR WAEAPRDLGY
60 70 80 90 100
SDFSPYQWRG LRMLKDPDTQ AVYHDMLWEL RPRTIVELGV YNGGSLAWFR
110 120 130 140 150
DLTKIMGIDC QVIGIDRDLS RCQIPASDME NITLHQGDCS DLTTFEHLRE
160 170 180 190 200
MAHPLIFIDD AHANTFNIMK WAVDHLLEEG DYFIIEDMIP YWYRYAPQLL
210 220 230
SEYLGAFRDV LSMDMLYANA SSQLDRGVLR RVAAKQ
Length:236
Mass (Da):27,584
Last modified:November 1, 1998 - v1
Checksum:iFD1EF1B650AF8070
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073896 Genomic DNA. Translation: AAC32491.1.

Genome annotation databases

PATRICi29741601. VBIStrCla15562_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073896 Genomic DNA. Translation: AAC32491.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BM8 X-ray 2.50 A/B/C/D/E/F/G/H/I/J/K/L 1-236 [» ]
2BM9 X-ray 2.94 A/B/C/D/E/F 1-236 [» ]
2BR3 X-ray 2.79 A/B/C/D/E/F 1-236 [» ]
2BR4 X-ray 2.59 A/B/C/D/E/F 1-236 [» ]
2BR5 X-ray 2.83 A/B/C/D/E/F 1-236 [» ]
ProteinModelPortali O85726.
SMRi O85726. Positions 2-234.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

PATRICi 29741601. VBIStrCla15562_0183.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13421.

Miscellaneous databases

EvolutionaryTracei O85726.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR007072. Rhamnosyl_O-MeTrfase_CmcI.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF04989. CmcI. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein."
    Alexander D.C., Jensen S.E.
    J. Bacteriol. 180:4068-4079(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NRRL 3585Imported.
  2. "Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus."
    Oster L.M., Lester D.R., Terwisscha van Scheltinga A., Svenda M., van Lun M., Genereux C., Andersson I.
    J. Mol. Biol. 358:546-558(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR(4FE-4S-S-ADOMET) AND S-ADENOSYL-L-HOMOCYSTEINE.

Entry informationi

Entry nameiO85726_STRCL
AccessioniPrimary (citable) accession number: O85726
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3