Cephalosporin hydroxylase CmcI - Streptomyces clavuligerus

Preferred order of sections

Names and origin

Protein names

Submitted name:
Cephalosporin hydroxylase CmcI EMBL AAC32491.1

Gene names

Name:

cmcI EMBL AAC32491.1

Organism

Streptomyces clavuligerus EMBL AAC32491.1

Taxonomic identifier

1901 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine PDB 2BM9 PDB 2BR4
Technical term	3D-structure PDB 2BM9 PDB 2BR4 PDB 2BR5 PDB 2BR3 PDB 2BM8
Gene Ontology (GO)
Biological_process	lipid biosynthetic process Inferred from electronic annotation. Source: InterPro methylation Inferred from electronic annotation. Source: GOC
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

64 – 65

2

S-adenosyl-L-homocysteine binding

Region

64 – 65

2

S-adenosyl-L-methionine binding PDB 2BM9 PDB 2BR4

Region

87 – 91

5

S-adenosyl-L-homocysteine binding

Region

87 – 91

5

S-adenosyl-L-methionine binding PDB 2BM9 PDB 2BR4

Region

116 – 117

2

S-adenosyl-L-homocysteine binding

Region

116 – 117

2

S-adenosyl-L-methionine binding PDB 2BM9 PDB 2BR4

Region

138 – 139

2

S-adenosyl-L-homocysteine binding

Region

138 – 139

2

S-adenosyl-L-methionine binding PDB 2BM9 PDB 2BR4

Region

160 – 161

2

S-adenosyl-L-methionine binding PDB 2BM9 PDB 2BR4

Sites

Binding site

17

1

S-adenosyl-L-methionine; via carbonyl oxygen PDB 2BM9

Binding site

95

1

S-adenosyl-L-homocysteine

Binding site

95

1

S-adenosyl-L-methionine PDB 2BM9 PDB 2BR4

Binding site

121

1

S-adenosyl-L-homocysteine

Binding site

121

1

S-adenosyl-L-methionine PDB 2BM9 PDB 2BR4

Binding site

161

1

S-adenosyl-L-homocysteine

Binding site

187

1

S-adenosyl-L-homocysteine

Binding site

187

1

S-adenosyl-L-methionine PDB 2BM9

Sequences

Sequence

Length

Mass (Da)

Tools

O85726 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: FD1EF1B650AF8070
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FASTA

236

27,584

        10         20         30         40         50         60 
MNDYSRQNFL DLNLFRGLGE DPAYHPPVLT DRPRDWPLDR WAEAPRDLGY SDFSPYQWRG 

        70         80         90        100        110        120 
LRMLKDPDTQ AVYHDMLWEL RPRTIVELGV YNGGSLAWFR DLTKIMGIDC QVIGIDRDLS 

       130        140        150        160        170        180 
RCQIPASDME NITLHQGDCS DLTTFEHLRE MAHPLIFIDD AHANTFNIMK WAVDHLLEEG 

       190        200        210        220        230 
DYFIIEDMIP YWYRYAPQLL SEYLGAFRDV LSMDMLYANA SSQLDRGVLR RVAAKQ

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References

[1]	"Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein." Alexander D.C., Jensen S.E. J. Bacteriol. 180:4068-4079(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NRRL 3585 EMBL AAC32491.1.
[2]	"Insights into cephamycin biosynthesis: the crystal structure of CmcI from Streptomyces clavuligerus." Oster L.M., Lester D.R., Terwisscha van Scheltinga A., Svenda M., van Lun M., Genereux C., Andersson I. J. Mol. Biol. 358:546-558(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND S-ADENOSYL-L-METHIONINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF073896 Genomic DNA. Translation: AAC32491.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BM8	X-ray	2.50	A/B/C/D/E/F/G/H/I/J/K/L	1-236	[»]
2BM9	X-ray	2.94	A/B/C/D/E/F	1-236	[»]
2BR3	X-ray	2.79	A/B/C/D/E/F	1-236	[»]
2BR4	X-ray	2.59	A/B/C/D/E/F	1-236	[»]
2BR5	X-ray	2.83	A/B/C/D/E/F	1-236	[»]

ProteinModelPortal

O85726.

SMR

O85726. Positions 2-234.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

PATRIC

29741601. VBIStrCla15562_0183.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-13421.

Family and domain databases

InterPro

IPR007072. Rhamnosyl_O-MeTrfase_CmcI.
[Graphical view]

Pfam

PF04989. CmcI. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O85726.

Entry information

Entry name

O85726_STRCL

Accession

Primary (citable) accession number: O85726

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)