Reviewed, UniProtKB/Swiss-Prot O85718 (GLYA_ACIRA)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase
      Short name=Serine methylase
      Short name=SHMT
    EC=2.1.2.1

Gene names

Name:

glyA

Organism

Acinetobacter radioresistens

Taxonomic identifier

40216 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase HAMAP MF_00051		PRO_0000113523
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O85718-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 1F3DF3B3A8D21D62	FASTA	417	45,033
	10 20 30 40 50 60 MFANISIAEF DPELAQAITN EDARQEAHIE LIASENYCSP AVMEAQGSKL TNKYAEGYPG 70 80 90 100 110 120 KRYYGGCEYV DIIEQLAIDR AKELFGADYA NVQPHAGSQA NSAVYLALLN PGDTVLGMSL 130 140 150 160 170 180 AHGGHLTHGA KVSFSGKTYN AIQYGLNPET GEIDYEEVER LALEHKPRMI VAGFSAYSQI 190 200 210 220 230 240 VDWQRFRDIA DKIGAYLFVD MAHVAGLVAA GVYPNPVQIA DVTTTTTHKT LRGPRSGLIL 250 260 270 280 290 300 AKANEEIEKK LQSAVFPGNQ GGPLVHAVAA KAICFKEAMA PEYKAYQQQV VKNAQAMAEV 310 320 330 340 350 360 LIERGYDVVS GGTKNHLFLL SLIKQDITGK DADAWLGAAH ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 IRIGTPAVTT RGFGEAEVRD LASWIADILD SKGDEAVINT VKAKVEAVCA KFPVYAK

References

[1]	"Cloning and complete nucleotide sequence of Acinetobacter radioresistens CMC-1 AglyA gene encoding serine hydroxymethyltransferase." Hong M.C., Wu M.L., Chang M.C. FEMS Microbiol. Lett. 170:413-418(1999) [PubMed: 9933935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CMC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF073953 Genomic DNA. Translation: AAQ13463.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
SMR	O85718. Positions 5-416.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.2.1. 191733.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_ACIRA

Accession

Primary (citable) accession number: O85718
Secondary accession number(s): Q4LDM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	September 22, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)