O85709 (RELA_STRAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I

Gene names

Name:

relA

Organism

Streptomyces antibioticus

Taxonomic identifier

1890 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity. Is required for actinomycin production.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 841	841	GTP pyrophosphokinase		PRO_0000166563
Regions
	Domain	146 – 243	98	HD
	Domain	762 – 835	74	ACT

Sequences

	Sequence		Length	Mass (Da)
	O85709 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 632A037BA4EF4C94	FASTA	841	93,672
	10 20 30 40 50 60 MPDEAQHLTA AKPDSAAGAA AEPAAHAQDG GGPVEHDRSA PADKPAERTR PKPAPPERPR 70 80 90 100 110 120 PPPPRARAAG QPAARSGGSS NRVRARLARL GVQRANLTHP VLEPLLRIVR ANDPKIETST 130 140 150 160 170 180 LRQIEKAYQV AERWHRGQKR KSGDPYITHP LAVTTILAEL GMDPATLMAG LLHDSREDTE 190 200 210 220 230 240 YGLDDLRRDF GDVVGLLVDG VTKLDKVKFG EAAQAETVRK MVVAMAKDPR VLVIKLADRL 250 260 270 280 290 300 HNMRTMRYLK REKQEKKARE TLEIYAPLAH RLGMNTIKWE LEDLAFAILY PKMYDEIVRL 310 320 330 340 350 360 VAERAPKRDE YLAIVTDEVQ SDLRARRIKA TVTGRPKHYY SVYQKIIVRG RDFAEIYDLV 370 380 390 400 410 420 GIRVLVDTVR DCYAALGTVH ARWNPVPGRF KDYIAMPKFN MYQSLHTTVI GPNGKPVELQ 430 440 450 460 470 480 IRTFDMHRRA EYGIAAHWKY KQEAVARASK VRTDVPKPAK GKDDHLNDMA WLRQLLDWQK 490 500 510 520 530 540 ETEDPGEFLE SLRFDLSRNE VFVFTPKSDV IALPAGATPV DFAYAVHTEV GHRTIGARVN 550 560 570 580 590 600 GRLVPLESTL DNGDLVEVFT SKAAGAGPSR DWLGFVKSPR ARNKIRAWFS KERRDEAIEQ 610 620 630 640 650 660 GKDAIARAMR KQNLPIQRIL TGDSLVTLAH EMRYPDISSS DAAIGEGHVG AQNVVQKLVQ 670 680 690 700 710 720 ALGGEEAASE EIDEAVPSRS RSRKRRSNQD PGVVVKGVDD VWVKLARCCT PVPGEPIIGF 730 740 750 760 770 780 VTRGSAVSVH RSDCVNVESL AREPERILEV EWAPTQSSVF LVAIQVEALD RSRLLSDVTR 790 800 810 820 830 840 VLSDQHVNIL SAAVQTSRDR VATSRFTFEM GDPKHLGHVL KAVRGVEGVY DVYRVTPGPQ P

References

[1]	"relA is required for actinomycin production in Streptomyces antibioticus." Hoyt S., Jones G.H. J. Bacteriol. 181:3824-3829(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 41481 / IMRU 3720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF072829 Genomic DNA. Translation: AAC26021.1.
3D structure databases
ProteinModelPortal	O85709.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR002912. ACT_dom. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_STRAT

Accession

Primary (citable) accession number: O85709

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program