O85672 (O85672_THETR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Glucoamylase EMBL AAC24003.1
EC=3.2.1.3 EMBL AAC24003.1

Gene names

Name:

ga1 EMBL AAC24003.1

Organism

Thermoanaerobacterium thermosaccharolyticum (Clostridium thermosaccharolyticum) EMBL AAC24003.1

Taxonomic identifier

1517 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Thermoanaerobacterium

Protein attributes

Sequence length	695 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosidase EMBL AAC24003.1 Hydrolase
Technical term	3D-structure PDB 1LF6 PDB 1LF9
Gene Ontology (GO)
Biological process	polysaccharide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Experimental info
	Non-terminal residue	695	1	EMBL AAC24003.1

Sequences

	Sequence		Length	Mass (Da)
	O85672 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 35429D5E359F0DD6	FASTA	695	77,585
	10 20 30 40 50 60 MSRKLIKYLP LLVLASSVLS GCSNNVSSIK IDRFNNISAV NGPGEEDTWA SAQKQGVGTA 70 80 90 100 110 120 NNYVSKVWFT LANGAISEVY YPTIDTADVK EIKFIVTDGK SFVPDETKDA ISKVEKFTDK 130 140 150 160 170 180 SLGYKLVNTD KKGRYRITKD ITDVKRNSLI MKAKFEALEG SIHDYKLYLA YDPHIKNQGS 190 200 210 220 230 240 YNEGYVIKAN NNEMLMAKRD NVYTALSSNI GWKGYSIGYY KVNDIMTDLD ENKQMTKHYD 250 260 270 280 290 300 SARGNIIEGA EIDLTKNSEF EIVLSFGGSD SEAAKTALET LGEDYNNLKN NYIDEWTKYC 310 320 330 340 350 360 NTLNNFNGKA NSLYYNSMMI LKASEDKTNK GAYIASLSIP WGDGQRDDNT GGYHLVWSRD 370 380 390 400 410 420 LYHVANAFIA AGDVDSANRS LDYLAKVVKD NGMIPQNTWI SGKPYWTGIQ LDEQADPIIL 430 440 450 460 470 480 SYRLKRYDLY DSLVKPLADF IIKIGPKTGQ ERWEEIGGYS PATMAAEVAG LTCAAYIAEQ 490 500 510 520 530 540 NKDYESAQKY QEKADNWQKL IDNLTYTENG PLGNGQYYIR IAGLSDPDAD FMINIANGGG 550 560 570 580 590 600 VYDQKEIVDP SFLELVRLGV KSADDPKILN TLKVVDSTIK VDTPKGPSWY RYNHDGYGEP 610 620 630 640 650 660 SKTELYHGAG KGRLWPLLTG ERGMYEIAAG KDATPYVKAM EKFANEGGII SEQVWEDTGL 670 680 690 PTDSASPLNW AHAEYVILFA SNIEHKVLDM PDIVY

References

[1]	"Glucoamylase from Thermoanaerobacterium thermosaccharolyticum: Sequence studies and analysis of the macromolecular architecture of the enzyme." Ducki A., Grundmann O., Konermann L., Mayer F., Hoppert M. J. Gen. Appl. Microbiol. 44:327-335(1998) [PubMed: 12501412] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Crystal structure and evolution of a prokaryotic glucoamylase." Aleshin A.E., Feng P.H., Honzatko R.B., Reilly P.J. J. Mol. Biol. 327:61-73(2003) [PubMed: 12614608] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 18-695.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF071548 Genomic DNA. Translation: AAC24003.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LF6	X-ray	2.10	A/B	18-695	[»]
1LF9	X-ray	2.20	A/B	18-695	[»]

ProteinModelPortal

O85672.

ModBase

Search...

Protein family/group databases

CAZy

GH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR006425. Glucoamylase_bac.
IPR015220. Glucodextran_N.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011613. Glyco_hydro_15.
[Graphical view]

Gene3D

G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.

Pfam

PF09137. Glucodextran_N. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]

SUPFAM

SSF74650. Gal_mut_like. 1 hit.
SSF48208. Glyco_trans_6hp. 1 hit.

TIGRFAMs

TIGR01535. Glucan_glucosid. 1 hit.

PROSITE

PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O85672_THETR

Accession

Primary (citable) accession number: O85672

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)