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O85465

- GUN5_BACAG

UniProt

O85465 - GUN5_BACAG

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Protein

Endoglucanase 5A

Gene

cel5A

Organism
Bacillus agaradhaerens (Bacillus agaradherans)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651Proton donor
Active sitei254 – 2541Nucleophile

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM5. Carbohydrate-Binding Module Family 5.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 5A (EC:3.2.1.4)
Alternative name(s):
Alkaline cellulase
Endo-1,4-beta-glucanase 5A
Gene namesi
Name:cel5A
OrganismiBacillus agaradhaerens (Bacillus agaradherans)
Taxonomic identifieri76935 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesSporolactobacillaceaeunclassified Sporolactobacillaceae

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Endoglucanase 5APRO_0000184043Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
400
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 355
Beta strandi39 – 413
Beta strandi44 – 463
Beta strandi56 – 616
Helixi63 – 664
Helixi67 – 693
Helixi72 – 809
Beta strandi86 – 949
Turni98 – 1003
Helixi104 – 11815
Beta strandi121 – 1277
Beta strandi129 – 1313
Turni134 – 1374
Helixi138 – 15215
Beta strandi158 – 1614
Turni173 – 1764
Helixi177 – 18913
Beta strandi193 – 1953
Beta strandi197 – 1993
Helixi202 – 2054
Helixi208 – 2125
Beta strandi221 – 2288
Turni229 – 2313
Helixi234 – 24512
Beta strandi250 – 2589
Beta strandi264 – 2663
Helixi268 – 28013
Beta strandi285 – 2917
Helixi312 – 3143
Helixi317 – 32812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3HX-ray1.57A30-329[»]
1E5JX-ray1.85A27-331[»]
1H11X-ray1.08A27-329[»]
1H2JX-ray1.15A27-329[»]
1H5VX-ray1.10A27-330[»]
1HF6X-ray1.15A27-329[»]
1OCQX-ray1.08A27-329[»]
1QHZX-ray1.95A27-331[»]
1QI0X-ray2.10A27-331[»]
1QI2X-ray1.75A27-331[»]
1W3KX-ray1.20A27-329[»]
1W3LX-ray1.04A27-329[»]
2A3HX-ray2.00A30-329[»]
2V38X-ray1.50A27-331[»]
3A3HX-ray1.64A30-329[»]
4A3HX-ray1.65A27-329[»]
5A3HX-ray1.82A27-329[»]
6A3HX-ray1.68A27-329[»]
7A3HX-ray0.95A27-329[»]
8A3HX-ray0.97A27-329[»]
ProteinModelPortaliO85465.
SMRiO85465. Positions 30-331.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO85465.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.10.10.20. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR003610. CBM_fam5/12.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02839. CBM_5_12. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM00495. ChtBD3. 1 hit.
[Graphical view]
SUPFAMiSSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85465-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKITTIFVV LLMTVALFSI GNTTAADNDS VVEEHGQLSI SNGELVNERG
60 70 80 90 100
EQVQLKGMSS HGLQWYGQFV NYESMKWLRD DWGINVFRAA MYTSSGGYID
110 120 130 140 150
DPSVKEKVKE AVEAAIDLDI YVIIDWHILS DNDPNIYKEE AKDFFDEMSE
160 170 180 190 200
LYGDYPNVIY EIANEPNGSD VTWGNQIKPY AEEVIPIIRN NDPNNIIIVG
210 220 230 240 250
TGTWSQDVHH AADNQLADPN VMYAFHFYAG THGQNLRDQV DYALDQGAAI
260 270 280 290 300
FVSEWGTSAA TGDGGVFLDE AQVWIDFMDE RNLSWANWSL THKDESSAAL
310 320 330 340 350
MPGANPTGGW TEAELSPSGT FVREKIRESA SIPPSDPTPP SDPGEPDPTP
360 370 380 390 400
PSDPGEYPAW DPNQIYTNEI VYHNGQLWQA KWWTQNQEPG DPYGPWEPLN
Length:400
Mass (Da):44,702
Last modified:November 1, 1998 - v1
Checksum:i3F9C66FB9BC36FFF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067428 Genomic DNA. Translation: AAC19169.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067428 Genomic DNA. Translation: AAC19169.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A3H X-ray 1.57 A 30-329 [» ]
1E5J X-ray 1.85 A 27-331 [» ]
1H11 X-ray 1.08 A 27-329 [» ]
1H2J X-ray 1.15 A 27-329 [» ]
1H5V X-ray 1.10 A 27-330 [» ]
1HF6 X-ray 1.15 A 27-329 [» ]
1OCQ X-ray 1.08 A 27-329 [» ]
1QHZ X-ray 1.95 A 27-331 [» ]
1QI0 X-ray 2.10 A 27-331 [» ]
1QI2 X-ray 1.75 A 27-331 [» ]
1W3K X-ray 1.20 A 27-329 [» ]
1W3L X-ray 1.04 A 27-329 [» ]
2A3H X-ray 2.00 A 30-329 [» ]
2V38 X-ray 1.50 A 27-331 [» ]
3A3H X-ray 1.64 A 30-329 [» ]
4A3H X-ray 1.65 A 27-329 [» ]
5A3H X-ray 1.82 A 27-329 [» ]
6A3H X-ray 1.68 A 27-329 [» ]
7A3H X-ray 0.95 A 27-329 [» ]
8A3H X-ray 0.97 A 27-329 [» ]
ProteinModelPortali O85465.
SMRi O85465. Positions 30-331.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM5. Carbohydrate-Binding Module Family 5.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei O85465.

Family and domain databases

Gene3Di 2.10.10.20. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR003610. CBM_fam5/12.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02839. CBM_5_12. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view ]
SMARTi SM00495. ChtBD3. 1 hit.
[Graphical view ]
SUPFAMi SSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Bjornvad M.E.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700163 / DSM 8721 / LMG 17948 / NCIMB 40482.
  2. "Structure of the Bacillus agaradherans family 5 endoglucanase at 1.6-A and its cellobiose complex at 2.0-A resolution."
    Davies G.J., Dauter M., Brzozowski A.M., Bjoernvad M.E., Andersen K.V., Schuelein M.
    Biochemistry 37:1926-1932(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 30-329.
    Strain: ATCC 700163 / DSM 8721 / LMG 17948 / NCIMB 40482.
  3. "Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase."
    Davies G.J., MacKenzie L.F., Varrot A., Dauter M., Brzozowski A.M., Schuelein M., Withers S.G.
    Biochemistry 37:11707-11713(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) OF 30-329.
    Strain: ATCC 700163 / DSM 8721 / LMG 17948 / NCIMB 40482.

Entry informationi

Entry nameiGUN5_BACAG
AccessioniPrimary (citable) accession number: O85465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3