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O85465

- GUN5_BACAG

UniProt

O85465 - GUN5_BACAG

Protein

Endoglucanase 5A

Gene

cel5A

Organism
Bacillus agaradhaerens (Bacillus agaradherans)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei165 – 1651Proton donor
    Active sitei254 – 2541Nucleophile

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM5. Carbohydrate-Binding Module Family 5.
    GH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 5A (EC:3.2.1.4)
    Alternative name(s):
    Alkaline cellulase
    Endo-1,4-beta-glucanase 5A
    Gene namesi
    Name:cel5A
    OrganismiBacillus agaradhaerens (Bacillus agaradherans)
    Taxonomic identifieri76935 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Endoglucanase 5APRO_0000184043Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    400
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi31 – 355
    Beta strandi39 – 413
    Beta strandi44 – 463
    Beta strandi56 – 616
    Helixi63 – 664
    Helixi67 – 693
    Helixi72 – 809
    Beta strandi86 – 949
    Turni98 – 1003
    Helixi104 – 11815
    Beta strandi121 – 1277
    Beta strandi129 – 1313
    Turni134 – 1374
    Helixi138 – 15215
    Beta strandi158 – 1614
    Turni173 – 1764
    Helixi177 – 18913
    Beta strandi193 – 1953
    Beta strandi197 – 1993
    Helixi202 – 2054
    Helixi208 – 2125
    Beta strandi221 – 2288
    Turni229 – 2313
    Helixi234 – 24512
    Beta strandi250 – 2589
    Beta strandi264 – 2663
    Helixi268 – 28013
    Beta strandi285 – 2917
    Helixi312 – 3143
    Helixi317 – 32812

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A3HX-ray1.57A30-329[»]
    1E5JX-ray1.85A27-331[»]
    1H11X-ray1.08A27-329[»]
    1H2JX-ray1.15A27-329[»]
    1H5VX-ray1.10A27-330[»]
    1HF6X-ray1.15A27-329[»]
    1OCQX-ray1.08A27-329[»]
    1QHZX-ray1.95A27-331[»]
    1QI0X-ray2.10A27-331[»]
    1QI2X-ray1.75A27-331[»]
    1W3KX-ray1.20A27-329[»]
    1W3LX-ray1.04A27-329[»]
    2A3HX-ray2.00A30-329[»]
    2V38X-ray1.50A27-331[»]
    3A3HX-ray1.64A30-329[»]
    4A3HX-ray1.65A27-329[»]
    5A3HX-ray1.82A27-329[»]
    6A3HX-ray1.68A27-329[»]
    7A3HX-ray0.95A27-329[»]
    8A3HX-ray0.97A27-329[»]
    ProteinModelPortaliO85465.
    SMRiO85465. Positions 30-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO85465.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.10.10.20. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR003610. CBM_fam5/12.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02839. CBM_5_12. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view]
    SMARTiSM00495. ChtBD3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51055. SSF51055. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O85465-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKITTIFVV LLMTVALFSI GNTTAADNDS VVEEHGQLSI SNGELVNERG    50
    EQVQLKGMSS HGLQWYGQFV NYESMKWLRD DWGINVFRAA MYTSSGGYID 100
    DPSVKEKVKE AVEAAIDLDI YVIIDWHILS DNDPNIYKEE AKDFFDEMSE 150
    LYGDYPNVIY EIANEPNGSD VTWGNQIKPY AEEVIPIIRN NDPNNIIIVG 200
    TGTWSQDVHH AADNQLADPN VMYAFHFYAG THGQNLRDQV DYALDQGAAI 250
    FVSEWGTSAA TGDGGVFLDE AQVWIDFMDE RNLSWANWSL THKDESSAAL 300
    MPGANPTGGW TEAELSPSGT FVREKIRESA SIPPSDPTPP SDPGEPDPTP 350
    PSDPGEYPAW DPNQIYTNEI VYHNGQLWQA KWWTQNQEPG DPYGPWEPLN 400
    Length:400
    Mass (Da):44,702
    Last modified:November 1, 1998 - v1
    Checksum:i3F9C66FB9BC36FFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067428 Genomic DNA. Translation: AAC19169.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067428 Genomic DNA. Translation: AAC19169.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A3H X-ray 1.57 A 30-329 [» ]
    1E5J X-ray 1.85 A 27-331 [» ]
    1H11 X-ray 1.08 A 27-329 [» ]
    1H2J X-ray 1.15 A 27-329 [» ]
    1H5V X-ray 1.10 A 27-330 [» ]
    1HF6 X-ray 1.15 A 27-329 [» ]
    1OCQ X-ray 1.08 A 27-329 [» ]
    1QHZ X-ray 1.95 A 27-331 [» ]
    1QI0 X-ray 2.10 A 27-331 [» ]
    1QI2 X-ray 1.75 A 27-331 [» ]
    1W3K X-ray 1.20 A 27-329 [» ]
    1W3L X-ray 1.04 A 27-329 [» ]
    2A3H X-ray 2.00 A 30-329 [» ]
    2V38 X-ray 1.50 A 27-331 [» ]
    3A3H X-ray 1.64 A 30-329 [» ]
    4A3H X-ray 1.65 A 27-329 [» ]
    5A3H X-ray 1.82 A 27-329 [» ]
    6A3H X-ray 1.68 A 27-329 [» ]
    7A3H X-ray 0.95 A 27-329 [» ]
    8A3H X-ray 0.97 A 27-329 [» ]
    ProteinModelPortali O85465.
    SMRi O85465. Positions 30-331.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM5. Carbohydrate-Binding Module Family 5.
    GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O85465.

    Family and domain databases

    Gene3Di 2.10.10.20. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR003610. CBM_fam5/12.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02839. CBM_5_12. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SMARTi SM00495. ChtBD3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51055. SSF51055. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Bjornvad M.E.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 700163 / DSM 8721 / LMG 17948 / NCIMB 40482.
    2. "Structure of the Bacillus agaradherans family 5 endoglucanase at 1.6-A and its cellobiose complex at 2.0-A resolution."
      Davies G.J., Dauter M., Brzozowski A.M., Bjoernvad M.E., Andersen K.V., Schuelein M.
      Biochemistry 37:1926-1932(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 30-329.
      Strain: ATCC 700163 / DSM 8721 / LMG 17948 / NCIMB 40482.
    3. "Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase."
      Davies G.J., MacKenzie L.F., Varrot A., Dauter M., Brzozowski A.M., Schuelein M., Withers S.G.
      Biochemistry 37:11707-11713(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) OF 30-329.
      Strain: ATCC 700163 / DSM 8721 / LMG 17948 / NCIMB 40482.

    Entry informationi

    Entry nameiGUN5_BACAG
    AccessioniPrimary (citable) accession number: O85465
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3