B-N-acetylhexosaminidase - Streptomyces plicatus

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Unreviewed, UniProtKB/TrEMBL O85361 (O85361_STRPL)

Last modified June 16, 2009. Version 35. History...

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Names and origin · Protein attributes · Ontologies · Sequences · References · Cross-references · Entry information

Names and origin

Protein names

Submitted name:
B-N-acetylhexosaminidase EMBL AAC38798.3

Gene names

Name:

hex EMBL AAC38798.3

Organism

Streptomyces plicatus EMBL AAC38798.3

Taxonomic identifier

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Ontologies

Gene Ontology (GO)

Biological process

carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

Molecular function

beta-N-acetylhexosaminidase activity

Inferred from electronic annotation. Source: InterPro

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequences

Sequence

Length

Mass (Da)

Tools

O85361-1 [UniParc].

Last modified March 1, 2001. Version 3.
Checksum: E1A004A460C3610C
Show »

506

55,194

        10         20         30         40         50         60 
MTTGAAPDRK APVRPTPLDR VIPAPASVDP GGAPYRITRG THIRVDDSRE ARRVGDYLAD 

        70         80         90        100        110        120 
LLRPATGYRL PVTAHGHGGI RLRLAGGPYG DEGYRLDSGP AGVTITARKA AGLFHGVQTL 

       130        140        150        160        170        180 
RQLLPPAVEK DSAQPGPWLV AGGTIEDTPR YAWRSAMLDV SRHFFGVDEV KRYIDRVARY 

       190        200        210        220        230        240 
KYNKLHLHLS DDQGWRIAID SWPRLATYGG STEVGGGPGG YYTKAEYKEI VRYAASRHLE 

       250        260        270        280        290        300 
VVPEIDMPGH TNAALASYAE LNCDGVAPPL YTGTKVGFSS LCVDKDVTYD FVDDVIGELA 

       310        320        330        340        350        360 
ALTPGRYLHI GGDEAHSTPK ADFVAFMKRV QPIVAKYGKT VVGWHQLAGA EPVEGALVQY 

       370        380        390        400        410        420 
WGLDRTGDAE KAEVAEAARN GTGLILSPAD RTYLDMKYTK DTPLGLSWAG YVEVQRSYDW 

       430        440        450        460        470        480 
DPAGYLPGAP ADAVRGVEAP LWTETLSDPD QLDYMAFPRL PGVAELGWSP ASTHDWDTYK 

       490        500 
VRLAAQAPYW EAAGIDFYRS PQVPWT

References

[1]	"Structural and functional characterization of Streptomyces plicatus beta-N-acetylhexosaminidase by comparative molecular modeling and site-directed mutagenesis." Mark B.L., Wasney G.A., Salo T.J., Khan A.R., Cao Z., Robbins P.W., James M.N., Triggs-Raine B.L. J. Biol. Chem. 273:19618-19624(1998) [PubMed: 9677388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Mark B.L. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase." Mark B.L., Vocadlo D.J., Knapp S., Triggs-Raine B.L., Withers S.G., James M.N. J. Biol. Chem. 276:10330-10337(2001) [PubMed: 11124970] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 3-506.
[4]	"Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state." Williams S.J., Mark B.L., Vocadlo D.J., James M.N., Withers S.G. J. Biol. Chem. 277:40055-40065(2002) [PubMed: 12171933] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 3-506.
+	Additional computationally mapped references.

Cross-references

Sequence databases

AF063001 Genomic DNA. Translation: AAC38798.3.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HP4	X-ray	2.20	A	3-506	[»]
1HP5	X-ray	2.10	A	3-506	[»]
1M01	X-ray	2.10	A	3-506	[»]
1M03	X-ray	1.90	A	3-506	[»]
1M04	X-ray	1.95	A	3-506	[»]

ModBase

Protein family/group databases

CAZy

GH20. Glycoside Hydrolase Family 20.

Family and domain databases

InterPro

IPR001540. Glyco_hydro_20.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

PANTHER

PTHR22600. Glyco_hydro_20. 1 hit.

Pfam

PF00728. Glyco_hydro_20. 1 hit.
[Graphical view]

PRINTS

PR00738. GLHYDRLASE20.

ProtoNet

Entry information

Entry name

O85361_STRPL

Accession

Primary (citable) accession number: O85361

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 35 of the entry and version 3 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names and origin · Protein attributes · Ontologies · Sequences · References · Cross-references · Entry information