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Protein

Phosphomannomutase

Gene

manB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen.

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphomannomutase (manB)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Phosphoserine intermediateBy similarity1
Metal bindingi98Magnesium; via phosphate groupBy similarity1
Metal bindingi245MagnesiumBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi249MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processLipopolysaccharide biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00126; UER00424
UPA00281

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase (EC:5.4.2.8)
Short name:
PMM
Gene namesi
Name:manB
Ordered Locus Names:Z3194, ECs2835
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001478191 – 456PhosphomannomutaseAdd BLAST456

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi155864.Z3194

Structurei

3D structure databases

ProteinModelPortaliO85343
SMRiO85343
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphohexose mutase family.Curated

Phylogenomic databases

eggNOGiENOG4107QKV Bacteria
COG1109 LUCA
HOGENOMiHOG000268679
KOiK01840
OMAiPTDNGIK

Family and domain databases

InterProiView protein in InterPro
IPR005844 A-D-PHexomutase_a/b/a-I
IPR016055 A-D-PHexomutase_a/b/a-I/II/III
IPR005845 A-D-PHexomutase_a/b/a-II
IPR005846 A-D-PHexomutase_a/b/a-III
IPR005843 A-D-PHexomutase_C
IPR036900 A-D-PHexomutase_C_sf
IPR016066 A-D-PHexomutase_CS
IPR005841 Alpha-D-phosphohexomutase_SF
PfamiView protein in Pfam
PF02878 PGM_PMM_I, 1 hit
PF02879 PGM_PMM_II, 1 hit
PF02880 PGM_PMM_III, 1 hit
PF00408 PGM_PMM_IV, 1 hit
PRINTSiPR00509 PGMPMM
SUPFAMiSSF53738 SSF53738, 3 hits
SSF55957 SSF55957, 1 hit
PROSITEiView protein in PROSITE
PS00710 PGM_PMM, 1 hit

Sequencei

Sequence statusi: Complete.

O85343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLTCFKAY DIRGKLGEEL NEDIAWRIGR AYGEFLKPKT IVLGGDVRLT
60 70 80 90 100
SEALKLALAK GLQDAGVDVL DIGMSGTEEI YFATFHLGVD GGIEVTASHN
110 120 130 140 150
PMDYNGMKLV REGARPISGD TGLRDVQRLA EANDFPPVDE TKRGRYQQIN
160 170 180 190 200
LRDAYVDHLF GYINVKNLTP LKLVINSGNG AAGPVVDAIE ARFKALGAPV
210 220 230 240 250
ELIKVHNTPD GNFPNGIPNP LLPECRDDTR NAVIKHGADM GIAFDGDFDR
260 270 280 290 300
CFLFDEKGQF IEGYYIVGLL AEAFLEKNPG AKIIHDPRLS WNTVDVVTAA
310 320 330 340 350
GGTPVMSKTG HAFIKERMRK EDAIYGGEMS AHHYFRDFAY CDSGMIPWLL
360 370 380 390 400
VAELVCLKGK TLGEMVRDRM AAFPASGEIN SKLAQPVEAI NRVEQHFSRE
410 420 430 440 450
ALAVDRTDGI SMTFADWRFN LRSSNTEPVV RLNVESRGDV KLMEKKTKAL

LKLLSE
Length:456
Mass (Da):50,340
Last modified:November 1, 1998 - v1
Checksum:i6845D9D2DD7628B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061251 Genomic DNA Translation: AAC32349.1
AB008676 Genomic DNA Translation: BAA77734.1
AE005174 Genomic DNA Translation: AAG57090.1
BA000007 Genomic DNA Translation: BAB36258.1
PIRiC90983
F85828
RefSeqiNP_310862.1, NC_002695.1
WP_000839208.1, NZ_MWVM01000008.1

Genome annotation databases

EnsemblBacteriaiAAG57090; AAG57090; Z3194
BAB36258; BAB36258; BAB36258
GeneIDi913941
KEGGiece:Z3194
ecs:ECs2835
PATRICifig|386585.9.peg.2968

Similar proteinsi

Entry informationi

Entry nameiMANB_ECO57
AccessioniPrimary (citable) accession number: O85343
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health