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O85343

- MANB_ECO57

UniProt

O85343 - MANB_ECO57

Protein

Phosphomannomutase

Gene

manB

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen.

    Catalytic activityi

    Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981Phosphoserine intermediateBy similarity
    Metal bindingi98 – 981Magnesium; via phosphate groupBy similarity
    Metal bindingi245 – 2451MagnesiumBy similarity
    Metal bindingi247 – 2471MagnesiumBy similarity
    Metal bindingi249 – 2491MagnesiumBy similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. phosphomannomutase activity Source: UniProtKB-EC

    GO - Biological processi

    1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
    2. O antigen biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-2797-MONOMER.
    ECOO157:MANB-MONOMER.
    UniPathwayiUPA00126; UER00424.
    UPA00281.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphomannomutase (EC:5.4.2.8)
    Short name:
    PMM
    Gene namesi
    Name:manB
    Ordered Locus Names:Z3194, ECs2835
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456PhosphomannomutasePRO_0000147819Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z3194.

    Structurei

    3D structure databases

    ProteinModelPortaliO85343.
    SMRiO85343. Positions 1-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phosphohexose mutase family.Curated

    Phylogenomic databases

    eggNOGiCOG1109.
    HOGENOMiHOG000268679.
    KOiK01840.
    OMAiRLAIICE.
    OrthoDBiEOG6W9X55.

    Family and domain databases

    Gene3Di3.30.310.50. 1 hit.
    3.40.120.10. 3 hits.
    InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
    IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
    IPR005845. A-D-PHexomutase_a/b/a-II.
    IPR005846. A-D-PHexomutase_a/b/a-III.
    IPR005843. A-D-PHexomutase_C.
    IPR016066. A-D-PHexomutase_CS.
    IPR005841. Alpha-D-phosphohexomutase_SF.
    [Graphical view]
    PfamiPF02878. PGM_PMM_I. 1 hit.
    PF02879. PGM_PMM_II. 1 hit.
    PF02880. PGM_PMM_III. 1 hit.
    PF00408. PGM_PMM_IV. 1 hit.
    [Graphical view]
    PRINTSiPR00509. PGMPMM.
    SUPFAMiSSF53738. SSF53738. 3 hits.
    SSF55957. SSF55957. 1 hit.
    PROSITEiPS00710. PGM_PMM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O85343-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSLTCFKAY DIRGKLGEEL NEDIAWRIGR AYGEFLKPKT IVLGGDVRLT    50
    SEALKLALAK GLQDAGVDVL DIGMSGTEEI YFATFHLGVD GGIEVTASHN 100
    PMDYNGMKLV REGARPISGD TGLRDVQRLA EANDFPPVDE TKRGRYQQIN 150
    LRDAYVDHLF GYINVKNLTP LKLVINSGNG AAGPVVDAIE ARFKALGAPV 200
    ELIKVHNTPD GNFPNGIPNP LLPECRDDTR NAVIKHGADM GIAFDGDFDR 250
    CFLFDEKGQF IEGYYIVGLL AEAFLEKNPG AKIIHDPRLS WNTVDVVTAA 300
    GGTPVMSKTG HAFIKERMRK EDAIYGGEMS AHHYFRDFAY CDSGMIPWLL 350
    VAELVCLKGK TLGEMVRDRM AAFPASGEIN SKLAQPVEAI NRVEQHFSRE 400
    ALAVDRTDGI SMTFADWRFN LRSSNTEPVV RLNVESRGDV KLMEKKTKAL 450
    LKLLSE 456
    Length:456
    Mass (Da):50,340
    Last modified:November 1, 1998 - v1
    Checksum:i6845D9D2DD7628B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061251 Genomic DNA. Translation: AAC32349.1.
    AB008676 Genomic DNA. Translation: BAA77734.1.
    AE005174 Genomic DNA. Translation: AAG57090.1.
    BA000007 Genomic DNA. Translation: BAB36258.1.
    PIRiC90983.
    F85828.
    RefSeqiNP_288536.1. NC_002655.2.
    NP_310862.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG57090; AAG57090; Z3194.
    BAB36258; BAB36258; BAB36258.
    GeneIDi913941.
    962089.
    KEGGiece:Z3194.
    ecs:ECs2835.
    PATRICi18355054. VBIEscCol44059_2728.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061251 Genomic DNA. Translation: AAC32349.1 .
    AB008676 Genomic DNA. Translation: BAA77734.1 .
    AE005174 Genomic DNA. Translation: AAG57090.1 .
    BA000007 Genomic DNA. Translation: BAB36258.1 .
    PIRi C90983.
    F85828.
    RefSeqi NP_288536.1. NC_002655.2.
    NP_310862.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali O85343.
    SMRi O85343. Positions 1-456.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z3194.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG57090 ; AAG57090 ; Z3194 .
    BAB36258 ; BAB36258 ; BAB36258 .
    GeneIDi 913941.
    962089.
    KEGGi ece:Z3194.
    ecs:ECs2835.
    PATRICi 18355054. VBIEscCol44059_2728.

    Phylogenomic databases

    eggNOGi COG1109.
    HOGENOMi HOG000268679.
    KOi K01840.
    OMAi RLAIICE.
    OrthoDBi EOG6W9X55.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00424 .
    UPA00281 .
    BioCyci ECOL386585:GJFA-2797-MONOMER.
    ECOO157:MANB-MONOMER.

    Family and domain databases

    Gene3Di 3.30.310.50. 1 hit.
    3.40.120.10. 3 hits.
    InterProi IPR005844. A-D-PHexomutase_a/b/a-I.
    IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
    IPR005845. A-D-PHexomutase_a/b/a-II.
    IPR005846. A-D-PHexomutase_a/b/a-III.
    IPR005843. A-D-PHexomutase_C.
    IPR016066. A-D-PHexomutase_CS.
    IPR005841. Alpha-D-phosphohexomutase_SF.
    [Graphical view ]
    Pfami PF02878. PGM_PMM_I. 1 hit.
    PF02879. PGM_PMM_II. 1 hit.
    PF02880. PGM_PMM_III. 1 hit.
    PF00408. PGM_PMM_IV. 1 hit.
    [Graphical view ]
    PRINTSi PR00509. PGMPMM.
    SUPFAMi SSF53738. SSF53738. 3 hits.
    SSF55957. SSF55957. 1 hit.
    PROSITEi PS00710. PGM_PMM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes."
      Wang L., Reeves P.R.
      Infect. Immun. 66:3545-3551(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: O157:H7 / C664-1992 / EHEC.
    2. "Analysis of the genes responsible for the O-antigen synthesis in enterohaemorrhagic Escherichia coli O157."
      Shimizu T., Yamasaki S., Tsukamoto T., Takeda Y.
      Microb. Pathog. 26:235-247(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: O157:H- / 184 / EHEC.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    4. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiMANB_ECO57
    AccessioniPrimary (citable) accession number: O85343
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3