O85297 (FTSI_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidoglycan synthase FtsI
EC=2.4.1.129

Alternative name(s):
Penicillin-binding protein 3
Short name=PBP-3
Peptidoglycan glycosyltransferase 3

Gene names

Name:	ftsI
Ordered Locus Names:	BUsg_216

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates By similarity.
Catalytic activity	(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Domain	Has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	penicillin binding Inferred from electronic annotation. Source: InterPro peptidoglycan glycosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Peptidoglycan synthase FtsI		PRO_0000195459
Regions
	Topological domain	1 – 18	18	Cytoplasmic Potential
	Transmembrane	19 – 39	21	Helical; Potential
	Topological domain	40 – 568	529	Extracellular Potential
Sites
	Active site	302	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O85297 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 533B30828687CEE1	FASTA	568	63,939
	10 20 30 40 50 60 MNLFKEKKIK KIIYINWRFV TLCSIVFLFL VILTLRIIFL QIINSKKLAY EGDRRTLRIQ 70 80 90 100 110 120 SVINRRGIIN DRLGYPLAVA VPVNAVFIDP TMITNKNDIK NNIRWKALSE ILSIPLNKLI 130 140 150 160 170 180 FFINSDKNIK FIYLARQINP EIGDYIKALK LPGVFLIEES KRYYPTGAIA AQLIGINNID 190 200 210 220 230 240 GEGIEGIEKS FNSYLTGTPG KRKIRKDNQG QIIENESLIN KSNSNNLILS IDKKLQTIVY 250 260 270 280 290 300 QKLNNAVNEN QADFGIAILI NIETGEILAM ANSPSYNPNN MQYMINKNLR NKAITDIFEP 310 320 330 340 350 360 GSTVKPIVIM EALKRGIIKK NSIINTKPYF IKKHKITDVA YHEKLNITGI LKKSSNVGVS 370 380 390 400 410 420 KIALSMNTSE LINSYIKFGL GQPTNLGLIG EQKGFLPKKK KLSDLEKATF SFGYGLMITP 430 440 450 460 470 480 LQLARLYTII GSYGIYRPLS IIKIDHPLYE KRIFPKRYVK NVIHMMETVA HPGEGGSQAA 490 500 510 520 530 540 IKGYRVAIKT GTAKKVGIHG YYIKKYIAYT AGIAPASNPK FSLTIIIDNP KGEKYYGGAV 550 560 SAPVFSKIMK LVLKEMKIKP DNLKNKLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes dapD-htrA-ilvI-ilvH-ftsL-ftsI-murE." Thao M.L., Baumann P. Curr. Microbiol. 37:214-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF060492 Genomic DNA. Translation: AAC32337.1. AE013218 Genomic DNA. Translation: AAM67776.1.
RefSeq	NP_660565.1. NC_004061.1.
3D structure databases
ProteinModelPortal	O85297.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg216.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67776; AAM67776; BUsg_216.
GeneID	1005415.
KEGG	bas:BUsg216.
PATRIC	21247227. VBIBucAph100086_0227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0768.
KO	K03587.
OMA	VECEGSI.
ProtClustDB	CLSK315801.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-278-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56519. PBP_dimer. 1 hit. SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FTSI_BUCAP

Accession

Primary (citable) accession number: O85297

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program