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O85297 (FTSI_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidoglycan synthase FtsI

EC=2.4.1.129
Alternative name(s):
Penicillin-binding protein 3
Short name=PBP-3
Peptidoglycan glycosyltransferase 3
Gene names
Name:ftsI
Ordered Locus Names:BUsg_216
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates By similarity.

Catalytic activity

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell membrane; Single-pass membrane protein Potential.

Domain

Has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Sequence similarities

Belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568Peptidoglycan synthase FtsI
PRO_0000195459

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 3921Helical; Potential
Topological domain40 – 568529Extracellular Potential

Sites

Active site3021Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
O85297 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 533B30828687CEE1

FASTA56863,939
        10         20         30         40         50         60 
MNLFKEKKIK KIIYINWRFV TLCSIVFLFL VILTLRIIFL QIINSKKLAY EGDRRTLRIQ 

        70         80         90        100        110        120 
SVINRRGIIN DRLGYPLAVA VPVNAVFIDP TMITNKNDIK NNIRWKALSE ILSIPLNKLI 

       130        140        150        160        170        180 
FFINSDKNIK FIYLARQINP EIGDYIKALK LPGVFLIEES KRYYPTGAIA AQLIGINNID 

       190        200        210        220        230        240 
GEGIEGIEKS FNSYLTGTPG KRKIRKDNQG QIIENESLIN KSNSNNLILS IDKKLQTIVY 

       250        260        270        280        290        300 
QKLNNAVNEN QADFGIAILI NIETGEILAM ANSPSYNPNN MQYMINKNLR NKAITDIFEP 

       310        320        330        340        350        360 
GSTVKPIVIM EALKRGIIKK NSIINTKPYF IKKHKITDVA YHEKLNITGI LKKSSNVGVS 

       370        380        390        400        410        420 
KIALSMNTSE LINSYIKFGL GQPTNLGLIG EQKGFLPKKK KLSDLEKATF SFGYGLMITP 

       430        440        450        460        470        480 
LQLARLYTII GSYGIYRPLS IIKIDHPLYE KRIFPKRYVK NVIHMMETVA HPGEGGSQAA 

       490        500        510        520        530        540 
IKGYRVAIKT GTAKKVGIHG YYIKKYIAYT AGIAPASNPK FSLTIIIDNP KGEKYYGGAV 

       550        560 
SAPVFSKIMK LVLKEMKIKP DNLKNKLS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a DNA fragment from Buchnera aphidicola (Aphid endosymbiont) containing the genes dapD-htrA-ilvI-ilvH-ftsL-ftsI-murE."
Thao M.L., Baumann P.
Curr. Microbiol. 37:214-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF060492 Genomic DNA. Translation: AAC32337.1.
AE013218 Genomic DNA. Translation: AAM67776.1.
RefSeqNP_660565.1. NC_004061.1.

3D structure databases

ProteinModelPortalO85297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67776; AAM67776; BUsg_216.
GeneID1005415.
KEGGbas:BUsg216.
PATRIC21247227. VBIBucAph100086_0227.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0768.
KOK03587.
OMAPDGKYIN.
OrthoDBEOG6N0HHV.
ProtClustDBCLSK315801.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-226-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFTSI_BUCAP
AccessionPrimary (citable) accession number: O85297
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names