Reviewed, UniProtKB/Swiss-Prot O85273 (NUOA_PECCC)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A
    NDH-1 subunit A

Gene names

Name:

nuoA

Organism

Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)

Taxonomic identifier

555 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Subunit structure	Composed of 13 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 146	146	NADH-quinone oxidoreductase subunit A		PRO_0000117869
Regions
	Transmembrane	14 – 34	21	Potential
	Transmembrane	68 – 88	21	Potential
	Transmembrane	96 – 116	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O85273-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D6B016B39D292AA6	FASTA	146	16,156
	10 20 30 40 50 60 MSTTTEILAH HWAFGLFLII AVGLCVFMLT GGFLLGGRAK GRAKNVPYES GIDSVGSARL 70 80 90 100 110 120 RLSAKFYLVA MFFVIFDVEA LYLYAWAVSI KESGWIGFIE ATIFILVLLA GLIYLVRVGA 130 140 LDWTPVRSKR QVVKSDIINT TNNHPQ

References

[1]

"The hexA gene of Erwinia carotovora encodes a LysR homologue and regulates motility and the expression of multiple virulence determinants." Harris S.J., Shih Y.L., Bentley S.D., Salmond G.P.C. Mol. Microbiol. 28:705-717(1998) [PubMed: 9643539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SCRI 193.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF057063 Genomic DNA. Translation: AAC38640.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000440. Oxidored_q4. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_PECCC

Accession

Primary (citable) accession number: O85273

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)