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Protein

Dihydrofolate reductase

Gene

dfr

Organism
Streptococcus pneumoniae
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dyr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dyr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfrIII), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR_2), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationImported

Keywords - Biological processi

One-carbon metabolismUniRule annotation

Keywords - Ligandi

NADPUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductaseUniRule annotation (EC:1.5.1.3UniRule annotation)
Gene namesi
Name:dfrImported
Synonyms:dhfRImported
ORF Names:ERS022016_01799Imported, ERS022329_01572Imported
OrganismiStreptococcus pneumoniaeImported
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000042611 Componenti: Unassembled WGS sequence
  • UP000042776 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 165162DHFR (dihydrofolate reductase)InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.UniRule annotation

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKIVAIWA QDEDGVIGKD NRLPWHLPAE LQHFKETTLN HAILMGRVTF
60 70 80 90 100
DGMGRRLLPK RETLILTRNS EEKLDGVATF HDVQSVLDWY HAQDKNLYIL
110 120 130 140 150
GGKQIFQAFE PYLDEVIVTH IHARVEGDTY FPEEFDLSLF ETVSSKSYTK
160
DEKNPYDFTI QYRKRKEV
Length:168
Mass (Da):19,673
Last modified:November 1, 1998 - v1
Checksum:i254937B7E21B7CBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055725 Genomic DNA. Translation: AAC33870.1.
CLIN01000026 Genomic DNA. Translation: CJK55047.1.
CMFG01000023 Genomic DNA. Translation: CJY51309.1.
RefSeqiWP_000162437.1. NZ_JFJA01000002.1.

Genome annotation databases

EnsemblBacteriaiCRE95648; CRE95648; ERS021697_01454.
CRF32927; CRF32927; ERS020326_00523.
CTF75958; CTF75958; ERS070163_01657.
CTF78665; CTF78665; ERS043984_01707.
CTF82749; CTF82749; ERS070165_01595.
CTM81632; CTM81632; ERS044097_01645.
CTM88513; CTM88513; ERS044099_01591.
CTO88625; CTO88625; ERS044091_01877.
CTP09311; CTP09311; ERS044127_01884.
CTP11055; CTP11055; ERS044103_01883.
CTP63499; CTP63499; ERS044148_01600.
KGI27123; KGI27123; BM49_0299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055725 Genomic DNA. Translation: AAC33870.1.
CLIN01000026 Genomic DNA. Translation: CJK55047.1.
CMFG01000023 Genomic DNA. Translation: CJY51309.1.
RefSeqiWP_000162437.1. NZ_JFJA01000002.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCRE95648; CRE95648; ERS021697_01454.
CRF32927; CRF32927; ERS020326_00523.
CTF75958; CTF75958; ERS070163_01657.
CTF78665; CTF78665; ERS043984_01707.
CTF82749; CTF82749; ERS070165_01595.
CTM81632; CTM81632; ERS044097_01645.
CTM88513; CTM88513; ERS044099_01591.
CTO88625; CTO88625; ERS044091_01877.
CTP09311; CTP09311; ERS044127_01884.
CTP11055; CTP11055; ERS044103_01883.
CTP63499; CTP63499; ERS044148_01600.
KGI27123; KGI27123; BM49_0299.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A conservative amino acid mutation in the chromosome-encoded dihydrofolate reductase confers trimethoprim resistance in Streptococcus pneumoniae."
    Pikis A., Donkersloot J.A., Rodriguez W.J., Keith J.M.
    J. Infect. Dis. 178:700-706(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: AP13Imported.
  2. Murphy D.
    Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SMRU2506Imported and SMRU2819Imported.

Entry informationi

Entry nameiO85262_STREE
AccessioniPrimary (citable) accession number: O85262
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.