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Protein

Dihydrofolate reductase

Gene

dfr

Organism
Streptococcus pneumoniae
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (AZJ08_03120), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (A5N45_02035), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (AZJ16_00375), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhfrIII), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (AWW74_09235), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (AZJ05_05095), Dihydrofolate reductase (dhfR_2), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (AZJ10_03960), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationImported
Biological processOne-carbon metabolismUniRule annotation
LigandNADPUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductaseUniRule annotation (EC:1.5.1.3UniRule annotation)
Gene namesi
Name:dfrImported
Synonyms:dhfRImported
ORF Names:CHL73_05245Imported, ERS020178_03992Imported, ERS020521_00188Imported, ERS021360_01095Imported, ERS021733_01923Imported, ERS367337_01484Imported
OrganismiStreptococcus pneumoniaeImported
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000071227 Componenti: Unassembled WGS sequence
  • UP000216187 Componenti: Unassembled WGS sequence
  • UP000039962 Componenti: Unassembled WGS sequence
  • UP000044493 Componenti: Unassembled WGS sequence
  • UP000041439 Componenti: Unassembled WGS sequence
  • UP000235698 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 165DHFRInterPro annotationAdd BLAST162

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYV Bacteria
COG0262 LUCA

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O85260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKIVAIWA QDEEGVIGKD NRLPWHLPAE LQHFKETTLN HAILMGRVTF
60 70 80 90 100
DGMGRRLLPK RETLILTRNP EEKIDGVATF QDVQSVLDWY QAQEKNLYII
110 120 130 140 150
GGKQIFQAFE PYLDEVIVTH IHARVEGDTY FPEELDLSLF ETVSSKFYAK
160
DEKNPYDFTI QYRKRKEV
Length:168
Mass (Da):19,689
Last modified:November 1, 1998 - v1
Checksum:i0D3886F2817D50E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055723 Genomic DNA Translation: AAC33868.1
CKTJ01000014 Genomic DNA Translation: CJA02437.1
CLUP01000016 Genomic DNA Translation: CJR88041.1
CQZO01000020 Genomic DNA Translation: COD59985.1
CGXO01000002 Genomic DNA Translation: COO82229.1
FEBA01000012 Genomic DNA Translation: CWE72131.1
NOIL01000028 Genomic DNA Translation: OZS30102.1
RefSeqiWP_000162452.1, NZ_NOIL01000028.1

Genome annotation databases

EnsemblBacteriaiCJA02437; CJA02437; ERS021360_01095
CJR88041; CJR88041; ERS021733_01923
COD59985; COD59985; ERS020178_03992
CWE72131; CWE72131; ERS367337_01484

Similar proteinsi

Entry informationi

Entry nameiO85260_STREE
AccessioniPrimary (citable) accession number: O85260
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health