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Protein

Dihydrofolate reductase

Gene

dfr

Organism
Streptococcus pneumoniae
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (NTPn46_07680), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhfrIII), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (AWW74_09235), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR_1), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR_2), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (BMJ42_01586), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhf), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (folA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dhfr), Dihydrofolate reductase (dhfR), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationImported
Biological processOne-carbon metabolismUniRule annotation
LigandNADPUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductaseUniRule annotation (EC:1.5.1.3UniRule annotation)
Gene namesi
Name:dfrImported
OrganismiStreptococcus pneumoniaeImported
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Interactioni

Protein-protein interaction databases

STRINGi170187.SpneT_02000377.

Structurei

3D structure databases

ProteinModelPortaliO85258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 165DHFR (dihydrofolate reductase)InterPro annotationAdd BLAST162

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYV. Bacteria.
COG0262. LUCA.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiView protein in InterPro
IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O85258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKIVAIWA QDEEGVIGKD NRLPWHLPAE LQHFKETTLN HAILMGRVTF
60 70 80 90 100
DGIGRRLLPQ RETLILTRNL EETIDGVATF HDVQSVLDWY QAQEKNLYIL
110 120 130 140 150
GGKQIFQAFE PYLDEVIVTH IHARVEGDTY FPEEFDLSLF ETVSSKFYAK
160
DEKNPYDFTI QYRKRKEV
Length:168
Mass (Da):19,703
Last modified:November 1, 1998 - v1
Checksum:i303917EBCD3AB0EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055721 Genomic DNA. Translation: AAC33864.1.
RefSeqiWP_000162441.1. NZ_FHIK01000001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055721 Genomic DNA. Translation: AAC33864.1.
RefSeqiWP_000162441.1. NZ_FHIK01000001.1.

3D structure databases

ProteinModelPortaliO85258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02000377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108YYV. Bacteria.
COG0262. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiView protein in InterPro
IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO85258_STREE
AccessioniPrimary (citable) accession number: O85258
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: January 18, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.