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O85222 (ANR_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional activator protein Anr

Short name=ANR
Alternative name(s):
Anaerobic regulatory protein
Gene names
Name:anr
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional activator of anaerobic gene expression. Regulates the expression of the components of the hydrogen cyanide synthase (HcnABC) in a positive manner. May also act as an iron sensor. Ref.1 Ref.2

Induction

Up-regulated by Fe3+ levels in oxygen-limiting conditions. Ref.1 Ref.2

Miscellaneous

Possesses 4 cysteines which may bind a metal ion (possibly iron) By similarity. UniProtKB P23926

Sequence similarities

Contains 1 cyclic nucleotide-binding domain.

Contains 1 HTH crp-type DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   LigandDNA-binding
Iron
   Molecular functionActivator
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244Transcriptional activator protein Anr
PRO_0000419765

Regions

Domain159 – 23274HTH crp-type
Nucleotide binding21 – 149129cNMP By similarity UniProtKB P23926
DNA binding192 – 21120H-T-H motif By similarity UniProtKB P0ACJ8

Sequences

Sequence LengthMass (Da)Tools
O85222 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 867AC251594A051F

FASTA24427,155
        10         20         30         40         50         60 
MSEPVKLRAH NQAHCKDCSL APLCLPLSLN LEDMDALDEI VKRGRPLKKG EFLFRQGDGF 

        70         80         90        100        110        120 
DSVYAVRSGA LKTFSLSDSG EEQITGFHLP SELVGLSGMD TESHPVSAQA LETTSVCEIP 

       130        140        150        160        170        180 
FERLDELALQ LPQLRRQLMR VMSREIRDDQ QMMLLLSKKT ADERIATFLV NLSARFRARG 

       190        200        210        220        230        240 
FSANQFRLSM SRNEIGNYLG LAVETVSRVF TRFQQNELIA AEGKEVHILD PIQLCALAGG 


SVEG 

« Hide

References

[1]"Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0."
Laville J., Blumer C., Von Schroetter C., Gaia V., Defago G., Keel C., Haas D.
J. Bacteriol. 180:3187-3196(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
Strain: CHA0.
[2]"Iron regulation of the hcnABC genes encoding hydrogen cyanide synthase depends on the anaerobic regulator ANR rather than on the global activator GacA in Pseudomonas fluorescens CHA0."
Blumer C., Haas D.
Microbiology 146:2417-2424(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
Strain: CHA0.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF053611 Genomic DNA. Translation: AAC38593.1.

3D structure databases

ProteinModelPortalO85222.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSPR00034. HTHCRP.
SMARTSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameANR_PSEFL
AccessionPrimary (citable) accession number: O85222
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families