Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Lactobacillus rhamnosus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA), GMP synthase (glutamine-hydrolyzing) (PZ01_10110), GMP synthase [glutamine-hydrolyzing] (guaA_2), GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881NucleophileBy similarity
Active sitei176 – 1761By similarity
Active sitei178 – 1781By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi230 – 2367ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.5.2. 2891.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
OrganismiLactobacillus rhamnosus
Taxonomic identifieri47715 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517GMP synthase [glutamine-hydrolyzing]PRO_0000140139Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi568703.LGG_01968.

Structurei

3D structure databases

ProteinModelPortaliO85192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 202192Glutamine amidotransferase type-1Add
BLAST
Domaini203 – 392190GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANDQNKDYD KIIVLDYGSQ YNQLITRRIR EFGIYSELKP HTITAAEVKK
60 70 80 90 100
IAPKGIIFSG GPNSVYDEGA LGVDEDIFKL GIPILGVCYG MQLMAQRLGG
110 120 130 140 150
DVEPADNREY GKADIEVTDA SAKLFHDLPK DQTVWMSHGD LVTRVPDGFR
160 170 180 190 200
TTATSVNCPI SAMDDDDRKF YGIQFHAEVQ NTQYGHEILH HFAFDVCHAE
210 220 230 240 250
ANWSMDDFIT KQIAKIRAEV GDKRVLLGLS GGVDSSVVGV LLHKAIGTQL
260 270 280 290 300
TSIFVDHGLL RKGEAEQVMD SLKGKFGLNI IKVNAKDRFL NDLRGVTDPE
310 320 330 340 350
KKRKIIGRDF IEVFNEEAAK LNGIEFLAQG TLYTDVVESG TDTAQTIKSH
360 370 380 390 400
HNVGGLPEDL KFKLIEPLNK LFKDEVRELG EKLGMPHSLV WRQPFPGPGL
410 420 430 440 450
GIRVIGEVTE DKLEIVRDSD YILREEIAKH GLDKDIWQYF TVLPGIRSVG
460 470 480 490 500
VMGDGRTYDY TIGIRAITSI DGMTADFARI DWDVLQEISS RIVNEVKHVN
510
RVVYDITSKP PATIEWE
Length:517
Mass (Da):57,792
Last modified:November 1, 1998 - v1
Checksum:i3A9D6EE91AE8B7DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051937 Genomic DNA. Translation: AAC33274.1.
RefSeqiWP_005712824.1. NZ_JPZB01000010.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051937 Genomic DNA. Translation: AAC33274.1.
RefSeqiWP_005712824.1. NZ_JPZB01000010.1.

3D structure databases

ProteinModelPortaliO85192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi568703.LGG_01968.

Protein family/group databases

MEROPSiC26.957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BRENDAi6.3.5.2. 2891.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_LACRH
AccessioniPrimary (citable) accession number: O85192
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.