Reviewed, UniProtKB/Swiss-Prot O85187 (NHAA_VIBCH)

Names and origin

Protein names

Recommended name:
    Na(+)/H(+) antiporter nhaA
Alternative name(s):
    Sodium/proton antiporter nhaA

Gene names

Name:	nhaA
Ordered Locus Names:	VC_1627

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Na+/H+ antiporter that extrudes sodium in exchange for external protons. Contributes to the survival of V.cholerae during the stationary and exponential growth phases in a saline environment. Ref.1 Ref.3
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Induction	Induced by sodium, lithium and potassium. Ref.1
Sequence similarities	Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family. [View classification]

Ontologies

Keywords
Biological process	Antiport Ion transport Sodium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	Sodium
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of pH Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	antiporter activity Inferred from electronic annotation. Source: HAMAP sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transmembrane transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Na(+)/H(+) antiporter nhaA HAMAP MF_01844		PRO_0000334456
Regions
	Transmembrane	47 – 67	21	Potential
	Transmembrane	87 – 107	21	Potential
	Transmembrane	117 – 137	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	171 – 191	21	Potential
	Transmembrane	205 – 225	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	281 – 303	23	Potential
	Transmembrane	321 – 341	21	Potential
	Transmembrane	353 – 373	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O85187-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 547A5E0586C525E4	FASTA	382	40,316
	10 20 30 40 50 60 MSDMIRDFFK MESAGGILLV IAAAIAMVIA NSAMGEGYQA FLHTYVFGMS VSHWINDGLM 70 80 90 100 110 120 AVFFLLIGLE VKRELLEGAL KSRETAIFPA IAAVGGMLAP ALIYVAFNFN DPAAIQGWAI 130 140 150 160 170 180 PAATDIAFAL GIMALLGKRV PVSLKVFLLA LAIIDDLGVV VIIALFYSSD LSTIALTIGF 190 200 210 220 230 240 IMTGVLFMLN AKHVTKLSIY LVAGLILWIA VLKSGVHATL AGVVIGFAIP LKGNKGEHSP 250 260 270 280 290 300 LKHLEHALHP YVAFAILPVF AFANAGISLQ GVSLAGLTSM LPLGVALGLF LGKPLGIFSF 310 320 330 340 350 360 SWAAVKLGVA KLPEGINFKH IFAVSVLCGI GFTMSIFISS LAFGQANEAY DTYARLGILM 370 380 GSTTAALLGY SLLRLSLPLK KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NhaA, an Na(+)/H(+) antiporter involved in environmental survival of Vibrio cholerae." Vimont S., Berche P. J. Bacteriol. 182:2937-2944(2000) [PubMed: 10781565] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS ANTIPORTER, INDUCTION. Strain: N18 / Serotype O1.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
[3]	"Roles of NhaA, NhaB, and NhaD Na(+)/H(+) antiporters in survival of Vibrio cholerae in a saline environment." Herz K., Vimont S., Padan E., Berche P. J. Bacteriol. 185:1236-1244(2003) [PubMed: 12562793] [Abstract] Cited for: FUNCTION. Strain: N18 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF051158 Genomic DNA. Translation: AAC33562.1. AE003852 Genomic DNA. Translation: AAF94778.1.
PIR	B82177.
RefSeq	NP_231264.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2613882.
GenomeReviews	Gene locus VC_1627 in contig AE003852_GR.
KEGG	vch:VC1627.
TIGR	VC_1627.
Phylogenomic databases
HOGENOM	O85187.
OMA	FFIAGLE.
Family and domain databases
HAMAP	MF_01844. [Tree]
InterPro	IPR004670. NhaA. [Graphical view]
Pfam	PF06965. Na_H_antiport_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00773. NhaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NHAA_VIBCH

Accession

Primary (citable) accession number: O85187
Secondary accession number(s): Q7DCU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)