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O85176 (ASSY_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Argininosuccinate synthase
EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Cgl1400, cg1586
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium ›

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148590

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site871Citrulline By similarity
Binding site1171ATP; via amide nitrogen By similarity
Binding site1191Aspartate By similarity
Binding site1231Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1241Aspartate By similarity
Binding site1271Citrulline By similarity
Binding site1751Citrulline By similarity
Binding site2591Citrulline By similarity
Binding site2711Citrulline By similarity

Experimental info

Sequence conflict1271R → G in AAC24818. Ref.1
Sequence conflict3041A → R in AAC24818. Ref.1
Sequence conflict3041A → R in AAB86624. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O85176 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: 271C63DD9C50CDFB

FASTA40144,135
        10         20         30         40         50         60 
MTNRIVLAYS GGLDTTVAIP YLKKMIDGEV IAVSLDLGQG GENMDNVRQR ALDAGAAESI 

        70         80         90        100        110        120 
VVDAKDEFAE EYCLPTIKAN GMYMKQYPLV SAISRPLIVK HLVEAGKQFN GTHVAHGCTG 

       130        140        150        160        170        180 
KGNDQVRFEV GFMDTDPNLE IIAPARDFAW TRDKAIAFAE ENNVPIEQSV KSPFSIDQNV 

       190        200        210        220        230        240 
WGRAIETGYL EDLWNAPTKD IYAYTEDPAL GNAPDEVIIS FEGGKPVSID GRPVSVLQAI 

       250        260        270        280        290        300 
EELNRRAGAQ GVGRLDMVED RLVGIKSREI YEAPGAIALI KAHEALEDVT IERELARYKR 

       310        320        330        340        350        360 
GVDARWAEEV YDGLWFGPLK RSLDAFIDST QEHVTGDIRM VLHAGSITIN GRRSSHSLYD 

       370        380        390        400 
FNLATYDTGD TFDQTLAKGF VQLHGLSSKI ANKRDREAGN N

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the arginine biosynthetic genes from Corynebacterium glutamicum."
Park M.Y., Chun J.Y., Ko S.-Y., Lee M.-S.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]"Molecular cloning of the argG gene from Corynebacterium glutamicum."
Ko S.-Y., Chun J.Y., Jung S.I., Lee M.-S.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[3]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF049897 Genomic DNA. Translation: AAC24818.1.
AF030520 Genomic DNA. Translation: AAB86624.1.
BA000036 Genomic DNA. Translation: BAB98793.1.
BX927152 Genomic DNA. Translation: CAF21411.1.
RefSeqNP_600619.1. NC_003450.3.
YP_225687.1. NC_006958.1.

3D structure databases

ProteinModelPortalO85176.
ModBaseSearch...

Protein-protein interaction databases

STRING196627.cg1586.

2D gel databases

World-2DPAGE0001:O85176.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB98793; BAB98793; BAB98793.
CAF21411; CAF21411; cg1586.
GeneID1019376.
3342925.
KEGGcgb:cg1586.
cgl:NCgl1346.
PATRIC21494839. VBICorGlu203724_1369.

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMANDQFRFE.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycCGLU196627:GJDM-1385-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR11587. PTHR11587. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_CORGL
AccessionPrimary (citable) accession number: O85176
Secondary accession number(s): O31144
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 11, 2002
Last modified: May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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