Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O85176

- ASSY_CORGL

UniProt

O85176 - ASSY_CORGL

Protein

Argininosuccinate synthase

Gene

argG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (11 Jul 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871CitrullineUniRule annotation
    Binding sitei117 – 1171ATP; via amide nitrogenUniRule annotation
    Binding sitei119 – 1191AspartateUniRule annotation
    Binding sitei123 – 1231AspartateUniRule annotation
    Binding sitei123 – 1231CitrullineUniRule annotation
    Binding sitei124 – 1241AspartateUniRule annotation
    Binding sitei127 – 1271CitrullineUniRule annotation
    Binding sitei175 – 1751CitrullineUniRule annotation
    Binding sitei259 – 2591CitrullineUniRule annotation
    Binding sitei271 – 2711CitrullineUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 169ATPUniRule annotation

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00113.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
    Alternative name(s):
    Citrulline--aspartate ligaseUniRule annotation
    Gene namesi
    Name:argGUniRule annotation
    Ordered Locus Names:Cgl1400, cg1586
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 401401Argininosuccinate synthasePRO_0000148590Add
    BLAST

    2D gel databases

    World-2DPAGE0001:O85176.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196627.cg1586.

    Structurei

    3D structure databases

    ProteinModelPortaliO85176.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0137.
    HOGENOMiHOG000230093.
    KOiK01940.
    OMAiKANALYM.
    OrthoDBiEOG6K9QCV.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O85176-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNRIVLAYS GGLDTTVAIP YLKKMIDGEV IAVSLDLGQG GENMDNVRQR    50
    ALDAGAAESI VVDAKDEFAE EYCLPTIKAN GMYMKQYPLV SAISRPLIVK 100
    HLVEAGKQFN GTHVAHGCTG KGNDQVRFEV GFMDTDPNLE IIAPARDFAW 150
    TRDKAIAFAE ENNVPIEQSV KSPFSIDQNV WGRAIETGYL EDLWNAPTKD 200
    IYAYTEDPAL GNAPDEVIIS FEGGKPVSID GRPVSVLQAI EELNRRAGAQ 250
    GVGRLDMVED RLVGIKSREI YEAPGAIALI KAHEALEDVT IERELARYKR 300
    GVDARWAEEV YDGLWFGPLK RSLDAFIDST QEHVTGDIRM VLHAGSITIN 350
    GRRSSHSLYD FNLATYDTGD TFDQTLAKGF VQLHGLSSKI ANKRDREAGN 400
    N 401
    Length:401
    Mass (Da):44,135
    Last modified:July 11, 2002 - v2
    Checksum:i271C63DD9C50CDFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti127 – 1271R → G in AAC24818. 1 PublicationCurated
    Sequence conflicti304 – 3041A → R in AAC24818. 1 PublicationCurated
    Sequence conflicti304 – 3041A → R in AAB86624. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF049897 Genomic DNA. Translation: AAC24818.1.
    AF030520 Genomic DNA. Translation: AAB86624.1.
    BA000036 Genomic DNA. Translation: BAB98793.1.
    BX927152 Genomic DNA. Translation: CAF21411.1.
    RefSeqiNP_600619.1. NC_003450.3.
    YP_225687.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB98793; BAB98793; BAB98793.
    CAF21411; CAF21411; cg1586.
    GeneIDi1019376.
    KEGGicgb:cg1586.
    cgl:NCgl1346.
    PATRICi21494839. VBICorGlu203724_1369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF049897 Genomic DNA. Translation: AAC24818.1 .
    AF030520 Genomic DNA. Translation: AAB86624.1 .
    BA000036 Genomic DNA. Translation: BAB98793.1 .
    BX927152 Genomic DNA. Translation: CAF21411.1 .
    RefSeqi NP_600619.1. NC_003450.3.
    YP_225687.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali O85176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg1586.

    2D gel databases

    World-2DPAGE 0001:O85176.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB98793 ; BAB98793 ; BAB98793 .
    CAF21411 ; CAF21411 ; cg1586 .
    GeneIDi 1019376.
    KEGGi cgb:cg1586.
    cgl:NCgl1346.
    PATRICi 21494839. VBICorGlu203724_1369.

    Phylogenomic databases

    eggNOGi COG0137.
    HOGENOMi HOG000230093.
    KOi K01940.
    OMAi KANALYM.
    OrthoDBi EOG6K9QCV.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the arginine biosynthetic genes from Corynebacterium glutamicum."
      Park M.Y., Chun J.Y., Ko S.-Y., Lee M.-S.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
    2. "Molecular cloning of the argG gene from Corynebacterium glutamicum."
      Ko S.-Y., Chun J.Y., Jung S.I., Lee M.-S.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
    3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiASSY_CORGL
    AccessioniPrimary (citable) accession number: O85176
    Secondary accession number(s): O31144
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 11, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3