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O85176

- ASSY_CORGL

UniProt

O85176 - ASSY_CORGL

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Protein

Argininosuccinate synthase

Gene

argG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871CitrullineUniRule annotation
Binding sitei117 – 1171ATP; via amide nitrogenUniRule annotation
Binding sitei119 – 1191AspartateUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei123 – 1231CitrullineUniRule annotation
Binding sitei124 – 1241AspartateUniRule annotation
Binding sitei127 – 1271CitrullineUniRule annotation
Binding sitei175 – 1751CitrullineUniRule annotation
Binding sitei259 – 2591CitrullineUniRule annotation
Binding sitei271 – 2711CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Cgl1400, cg1586
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Argininosuccinate synthasePRO_0000148590Add
BLAST

2D gel databases

World-2DPAGE0001:O85176.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg1586.

Structurei

3D structure databases

ProteinModelPortaliO85176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiKANALYM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85176-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNRIVLAYS GGLDTTVAIP YLKKMIDGEV IAVSLDLGQG GENMDNVRQR
60 70 80 90 100
ALDAGAAESI VVDAKDEFAE EYCLPTIKAN GMYMKQYPLV SAISRPLIVK
110 120 130 140 150
HLVEAGKQFN GTHVAHGCTG KGNDQVRFEV GFMDTDPNLE IIAPARDFAW
160 170 180 190 200
TRDKAIAFAE ENNVPIEQSV KSPFSIDQNV WGRAIETGYL EDLWNAPTKD
210 220 230 240 250
IYAYTEDPAL GNAPDEVIIS FEGGKPVSID GRPVSVLQAI EELNRRAGAQ
260 270 280 290 300
GVGRLDMVED RLVGIKSREI YEAPGAIALI KAHEALEDVT IERELARYKR
310 320 330 340 350
GVDARWAEEV YDGLWFGPLK RSLDAFIDST QEHVTGDIRM VLHAGSITIN
360 370 380 390 400
GRRSSHSLYD FNLATYDTGD TFDQTLAKGF VQLHGLSSKI ANKRDREAGN

N
Length:401
Mass (Da):44,135
Last modified:July 11, 2002 - v2
Checksum:i271C63DD9C50CDFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271R → G in AAC24818. 1 PublicationCurated
Sequence conflicti304 – 3041A → R in AAC24818. 1 PublicationCurated
Sequence conflicti304 – 3041A → R in AAB86624. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF049897 Genomic DNA. Translation: AAC24818.1.
AF030520 Genomic DNA. Translation: AAB86624.1.
BA000036 Genomic DNA. Translation: BAB98793.1.
BX927152 Genomic DNA. Translation: CAF21411.1.
RefSeqiNP_600619.1. NC_003450.3.
YP_225687.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98793; BAB98793; BAB98793.
CAF21411; CAF21411; cg1586.
GeneIDi1019376.
3342925.
KEGGicgb:cg1586.
cgl:NCgl1346.
PATRICi21494839. VBICorGlu203724_1369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF049897 Genomic DNA. Translation: AAC24818.1 .
AF030520 Genomic DNA. Translation: AAB86624.1 .
BA000036 Genomic DNA. Translation: BAB98793.1 .
BX927152 Genomic DNA. Translation: CAF21411.1 .
RefSeqi NP_600619.1. NC_003450.3.
YP_225687.1. NC_006958.1.

3D structure databases

ProteinModelPortali O85176.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg1586.

2D gel databases

World-2DPAGE 0001:O85176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB98793 ; BAB98793 ; BAB98793 .
CAF21411 ; CAF21411 ; cg1586 .
GeneIDi 1019376.
3342925.
KEGGi cgb:cg1586.
cgl:NCgl1346.
PATRICi 21494839. VBICorGlu203724_1369.

Phylogenomic databases

eggNOGi COG0137.
HOGENOMi HOG000230093.
KOi K01940.
OMAi KANALYM.
OrthoDBi EOG6K9QCV.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the arginine biosynthetic genes from Corynebacterium glutamicum."
    Park M.Y., Chun J.Y., Ko S.-Y., Lee M.-S.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  2. "Molecular cloning of the argG gene from Corynebacterium glutamicum."
    Ko S.-Y., Chun J.Y., Jung S.I., Lee M.-S.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiASSY_CORGL
AccessioniPrimary (citable) accession number: O85176
Secondary accession number(s): O31144
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 11, 2002
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3