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Protein

Argininosuccinate synthase

Gene

argG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871CitrullineUniRule annotation
Binding sitei117 – 1171ATP; via amide nitrogenUniRule annotation
Binding sitei119 – 1191AspartateUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei123 – 1231CitrullineUniRule annotation
Binding sitei124 – 1241AspartateUniRule annotation
Binding sitei127 – 1271CitrullineUniRule annotation
Binding sitei175 – 1751CitrullineUniRule annotation
Binding sitei259 – 2591CitrullineUniRule annotation
Binding sitei271 – 2711CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Cgl1400, cg1586
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Argininosuccinate synthasePRO_0000148590Add
BLAST

2D gel databases

World-2DPAGE0001:O85176.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg1586.

Structurei

3D structure databases

ProteinModelPortaliO85176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O85176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRIVLAYS GGLDTTVAIP YLKKMIDGEV IAVSLDLGQG GENMDNVRQR
60 70 80 90 100
ALDAGAAESI VVDAKDEFAE EYCLPTIKAN GMYMKQYPLV SAISRPLIVK
110 120 130 140 150
HLVEAGKQFN GTHVAHGCTG KGNDQVRFEV GFMDTDPNLE IIAPARDFAW
160 170 180 190 200
TRDKAIAFAE ENNVPIEQSV KSPFSIDQNV WGRAIETGYL EDLWNAPTKD
210 220 230 240 250
IYAYTEDPAL GNAPDEVIIS FEGGKPVSID GRPVSVLQAI EELNRRAGAQ
260 270 280 290 300
GVGRLDMVED RLVGIKSREI YEAPGAIALI KAHEALEDVT IERELARYKR
310 320 330 340 350
GVDARWAEEV YDGLWFGPLK RSLDAFIDST QEHVTGDIRM VLHAGSITIN
360 370 380 390 400
GRRSSHSLYD FNLATYDTGD TFDQTLAKGF VQLHGLSSKI ANKRDREAGN

N
Length:401
Mass (Da):44,135
Last modified:July 11, 2002 - v2
Checksum:i271C63DD9C50CDFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271R → G in AAC24818 (Ref. 1) Curated
Sequence conflicti304 – 3041A → R in AAC24818 (Ref. 1) Curated
Sequence conflicti304 – 3041A → R in AAB86624 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049897 Genomic DNA. Translation: AAC24818.1.
AF030520 Genomic DNA. Translation: AAB86624.1.
BA000036 Genomic DNA. Translation: BAB98793.1.
BX927152 Genomic DNA. Translation: CAF21411.1.
RefSeqiNP_600619.1. NC_003450.3.
WP_003858678.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98793; BAB98793; BAB98793.
CAF21411; CAF21411; cg1586.
GeneIDi1019376.
KEGGicgb:cg1586.
cgl:NCgl1346.
PATRICi21494839. VBICorGlu203724_1369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049897 Genomic DNA. Translation: AAC24818.1.
AF030520 Genomic DNA. Translation: AAB86624.1.
BA000036 Genomic DNA. Translation: BAB98793.1.
BX927152 Genomic DNA. Translation: CAF21411.1.
RefSeqiNP_600619.1. NC_003450.3.
WP_003858678.1. NC_006958.1.

3D structure databases

ProteinModelPortaliO85176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1586.

2D gel databases

World-2DPAGE0001:O85176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98793; BAB98793; BAB98793.
CAF21411; CAF21411; cg1586.
GeneIDi1019376.
KEGGicgb:cg1586.
cgl:NCgl1346.
PATRICi21494839. VBICorGlu203724_1369.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the arginine biosynthetic genes from Corynebacterium glutamicum."
    Park M.Y., Chun J.Y., Ko S.-Y., Lee M.-S.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  2. "Molecular cloning of the argG gene from Corynebacterium glutamicum."
    Ko S.-Y., Chun J.Y., Jung S.I., Lee M.-S.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiASSY_CORGL
AccessioniPrimary (citable) accession number: O85176
Secondary accession number(s): O31144
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 11, 2002
Last modified: July 22, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.