O85166 (O85166_BRUAO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cyclic beta 1-2 glucan synthase EMBL AAC34747.2

Gene names

Name:

cgs EMBL AAC34747.2

Organism

Brucella abortus EMBL AAC34747.2

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	2867 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro sphingolipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucosylceramidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O85166 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: D972F658E75A7F79	FASTA	2,867	320,464
	10 20 30 40 50 60 MPELSSSLSN SSTEHKTSEQ IPPEQPSPIR AHYKTEEELS VLAAAVARGE DIPLPGYLAF 70 80 90 100 110 120 PFDQRLSENG KLILHAYRAS DAASRAGETI TPAAQWLLDN HYQIDKNVQQ ARRDLPRRFI 130 140 150 160 170 180 RQLPLYKVPL EQYEGMPRIF ALAWLYVAHT DSNFSLKMLT AMVKGFQTVE PLKIGELWAL 190 200 210 220 230 240 PSAVRYFLIE NARRLAMRVD RAREMRNLAN TAADRIVVAA EGAELDGILA QYASAACDST 250 260 270 280 290 300 FATHLLYRLR SSSTDTTAAA SWLERILEQD GSNPEDAIIE EHARQSTGGV TMGNVIRSLK 310 320 330 340 350 360 SIDDVDWETW FETVNEVDAI LRDHSDFELL DFRSRNAYRV TIEKLARYSD MSEIDITWAA 370 380 390 400 410 420 LKLAEDGSRA EKAGHPEAEG KGAISYYLSG EGRRDLEKVC GYRAPFGVKA LRLYRGLGLW 430 440 450 460 470 480 GLFVPTAIFT VLTLLLVNYC MMRAGLSTDL RTLFTLLAIF PAMDASYSLF NVLVPWFVQP 490 500 510 520 530 540 TRLIGFEYKD GLPPEARTLV AVPTFITSRD SIDEQIRNLE VHYLTNPRGE IYFALVSDWT 550 560 570 580 590 600 DASQEIVPAD MEIYEYARDE IAKLNEHYTG DDQPRFFLLH RRRLFNEKQG CWMGWERKRG 610 620 630 640 650 660 KLHELNLLLR GDQDTSFFPA DERLPKDIKY VMTLDADTRL TPEAVTHLAG KLNHPMNRPV 670 680 690 700 710 720 IDEKTGKVVR GYGILQPRVT PSLTTGDEAS FLQRVFSVNR GIDPYVFAVS DTYQDLLGEG 730 740 750 760 770 780 TFTGKGLYDI DAFEAALKGR VPENTLLSHD LLEGGYARSA LVSDVEVVED YPTGYNVDVS 790 800 810 820 830 840 RHHRWARGDW QLLPWLFSTG RGVSAITRWK MVDNLRRSLN PILWLIAAIV GWSVLPLGAA 850 860 870 880 890 900 AVWQGFLILS MFVAPTFGIV TNLIPASMDY SLKGHAQSVL TDIALGTADV ALRTTFIAHS 910 920 930 940 950 960 AFLMADAIAR TIYRLFISRR NLLEWRTAAQ ARTSPDTLLF YFQLMWPAMA IAGLAIFFPL 970 980 990 1000 1010 1020 AAESHAALIA APFVVIWFAS PLIAWLVSRS AKPQDTLEVL SSDKADLRRY ARRTWRYFAE 1030 1040 1050 1060 1070 1080 FANEENNHLP PDNFQEDPAP IVAQRTSPTN IGVYLLSVIA ARDFGWIGFD ETLDRVEATL 1090 1100 1110 1120 1130 1140 GSLDKMEKYQ GHLYNWYETN TLNPMRPLYV SAVDSGNLAG HLVTLSSALE EWAEAPSVYV 1150 1160 1170 1180 1190 1200 QGDLDGIIDV NDILEETIAR IPDDRRVLRP LRRRLEERIA NFRRAVTSIK DEPETASFRT 1210 1220 1230 1240 1250 1260 INLSLFASDI VRLMDELDSE VETVASAEAR EWARILVDTC KAHTDDAIGE HDTDALRERL 1270 1280 1290 1300 1310 1320 RDLATRARQL AFDMQFGFLE RKERRLLSIG FRVQENELDE SCYDLLASEA RLASLFAIAK 1330 1340 1350 1360 1370 1380 GDVPVEHWFK LGRLLVPVGW KGALLSWSGS MFEYLMPPLV MSEPLGSLLD QTNKLVVQRQ 1390 1400 1410 1420 1430 1440 IDYATSRDLP WGISEAAFNA RDAHLNYQYS NFGVPNLGLQ RGLSRNAVIA PYASLLAAQY 1450 1460 1470 1480 1490 1500 QPAAAVANLK RLAKLGALGR YGFHDSVDFT PSRIREGETC AVVKNYYAHH HGMSIIAVNN 1510 1520 1530 1540 1550 1560 VIFEGRMRER FHNDPVIEAA ELLLQEKAPR EIPMIYAKTE NPMRVDSGGF DDAPMRIIDK 1570 1580 1590 1600 1610 1620 PFRAPRTTHM MSNGQYALMV TANGSGYSRW HNWDITRFHA DASEDMQGTF LFLRDMESGY 1630 1640 1650 1660 1670 1680 WWSATGEPVR NPDEETKTVF TEDKAEFYKT AGDIKTVMEV IVASEADGEG RRLDIINTSP 1690 1700 1710 1720 1730 1740 RDRMIEVTSY AELVLNDSDS DAAHPAFAKM FVETEIADSG STIYAKRRKR APSAPDIHAA 1750 1760 1770 1780 1790 1800 HFVTDLSGAL REIEVETDRR AFIGRGRSLR NAAAFDPGAK FTGSQGCVLD PIVSVRTRVR 1810 1820 1830 1840 1850 1860 VPAHKKVSLV FWTFAGGSRD AVEHAVTMHR HPETFAREYS LSWTSSQVQL YHIGIKPAEA 1870 1880 1890 1900 1910 1920 ADYQKVAAYL LYPERTLRQP PETIASGLGK QSDLWPMSIS GDYPIFALRI DNEADLDVLR 1930 1940 1950 1960 1970 1980 GVLRAQEYWR SKGLIVDVVA VNERAFSYAQ DTQRAIDWIV EGFRARGGGQ PHIFAVRRDQ 1990 2000 2010 2020 2030 2040 MSEESYNTLL ASARIVMHAQ NGSLAEQLRR SEEITVDLAA RSDARTATNG QASVQGLGAA 2050 2060 2070 2080 2090 2100 GLAPVAIERG AEERRPVVKP PVDRPRPSGD DLRFWNGYGG FAEDGSYVVR INGRTSTPHP 2110 2120 2130 2140 2150 2160 WINVIANPSF GFHVSAEGSA FTWAGNSRDY QLTPWANDPV TNRPGEAIYI LDRETGRSFA 2170 2180 2190 2200 2210 2220 PTANVLRDEA VTYEARHGHG YSAFSAQHGE LALELTHIVD AEKPVRLSRL TITNKGRSKR 2230 2240 2250 2260 2270 2280 KLRVYGYVEW VLGNARARNV PFIVPSQDEE LGALFAGNPY HPDKSGQVAF FAASEKPQSV 2290 2300 2310 2320 2330 2340 TADRGEFIGT AGSVDRPEIV LAGKVLSGTV EAGRDPCAAL ALDIEVGPGE AREIVFLLGN 2350 2360 2370 2380 2390 2400 TADQKQAKAL IADMRTASFE EKLVAARHQW ESFFNRVQVK TPDPAFDLMV NGWLPYQAVA 2410 2420 2430 2440 2450 2460 CRIWARAAFY QVSGAFGFRD QLQDTLSLLL VDPSLAREQI LNAASRQFRE GDVQHWWLPA 2470 2480 2490 2500 2510 2520 TGAGVRTLIS DDVVWLGYGA SLYVETTGDR FILDEKLPFL EGRKLEEGEH DAFYEPEISK 2530 2540 2550 2560 2570 2580 ETASLYEHCA LALDLAVART GKHGLPLILG GDWNDGMNLV GVKGKGESVW LGWFLAYTLK 2590 2600 2610 2620 2630 2640 AFIPLAEARK DQKRADLWKA HVESLTAALD RDGWDGEWYR RGFYDNGRPL GSKNSDECQI 2650 2660 2670 2680 2690 2700 DAIAQSWSVL SGVADPKRAK KAMASLEKYL LDEDNALLRL FTPPFDVTPQ EPGYIKGYPP 2710 2720 2730 2740 2750 2760 GVRENGGQYT HGATWAILAL ARMGKAAEAW RLFSLISPVS HGRNPDVYRV EPYVIAADIY 2770 2780 2790 2800 2810 2820 SVAPRCGQGG WTWYTGSAGW FYRVATEGIL GVTKRGDRLH FQPALPPEWE GYEAELRFGE 2830 2840 2850 2860 ALYRVKVVAG AKAGTIRLNG RKLAKPDEGV ALKPYGEHEI VVELPKQ

References

[1]	"Molecular cloning and characterization of cgs, the Brucella abortus cyclic beta(1-2) glucan synthetase gene: genetic complementation of Rhizobium meliloti ndvB and Agrobacterium tumefaciens chvB mutants." Inon de Iannino N., Briones G., Tolmasky M., Ugalde R.A. J. Bacteriol. 180:4392-4400(1998) [PubMed: 9721274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S19 EMBL AAC34747.2.
[2]	"Identification of active site residues of the inverting glycosyltransferase Cgs required for the synthesis of cyclic beta-1,2-glucan, a Brucella abortus virulence factor." Ciocchini A.E., Roset M.S., Briones G., Inon de Iannino N., Ugalde R.A. Glycobiology 16:679-691(2006) [PubMed: 16603625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S19 EMBL AAC34747.2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF047823 Genomic DNA. Translation: AAC34747.2.
PIR	T31419.
3D structure databases
ProteinModelPortal	O85166.
ModBase	Search...
Protein family/group databases
CAZy	GH94. Glycoside Hydrolase Family 94. GT84. Glycosyltransferase Family 84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR009342. Carb-bd_put_dom. IPR019282. DUF2329. IPR021478. DUF3131. IPR006775. Glucosylceramidase. IPR011013. Glyco_hydro-type_carb-bd. IPR010383. Glyco_transf_36. IPR010403. GT36_AF. [Graphical view]
Pfam	PF06204. CBM_X. 2 hits. PF10091. DUF2329. 1 hit. PF11329. DUF3131. 1 hit. PF04685. DUF608. 1 hit. PF06165. Glyco_transf_36. 2 hits. PF06205. GT36_AF. 2 hits. [Graphical view]
SMART	SM01068. CBM_X. 2 hits. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 2 hits. SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

O85166_BRUAO

Accession

Primary (citable) accession number: O85166

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)