O85078 (CBAD1_ARTSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-chlorobenzoyl coenzyme A dehalogenase-1
Short name=4-CBA-CoA dehalogenase-1
Short name=4-CBCoA dehalogenase-1
Short name=4-chlorobenzoyl-CoA dehalogenase-1
EC=3.8.1.7

Gene names

Name:

fcbB1

Organism

Arthrobacter sp.

Taxonomic identifier

1667 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dehalogenates 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA, 4-bromobenzoyl-CoA and, at a slower rate, 4-fluorobenzoyl-CoA. Does not dehalogenate 2-chlorobenzoyl-CoA or 3-chlorobenzoyl-CoA. Ref.2
Catalytic activity	4-chlorobenzoyl-CoA + H2O = 4-hydroxybenzoyl CoA + chloride. Ref.2 Ref.3
Pathway	Xenobiotic degradation; 4-chlorobenzoate degradation; 4-hydroxybenzoate from 4-chlorobenzoate: step 2/3. UniProtKB A5JTM5
Subunit structure	Homotetramer. Ref.2 Ref.3
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.
Biophysicochemical properties	Kinetic parameters: KM=14 µM for 4-iodobenzoyl-CoA (at 30 degrees Celsius, in 20 mM phosphate buffer, pH 7.2) Ref.2 Ref.3 Ref.4 KM=36 µM for 4-bromobenzoyl-CoA (at 30 degrees Celsius, in 20 mM phosphate buffer, pH 7.2) KM=34 µM for 4-chlorobenzoyl-CoA (at 30 degrees Celsius, in 20 mM phosphate buffer, pH 7.2) Ref.2 KM=75 µM for 4-fluorobenzoyl-CoA (at 30 degrees Celsius, in 20 mM phosphate buffer, pH 7.2) KM=9 µM for 4-chlorobenzoyl-CoA (at 30 degrees Celsius, in 50 mM phosphate buffer, pH 7.5) Ref.3 pH dependence: Optimum pH is 8.0 in MOPS buffer at 30 degrees Celsius, 7.5 in 0.1 M potassium phosphate buffer at 30 degrees Celsius, and 7.4 in 20 mM potassium phosphate/2 mM DTT buffer at 30 degrees Celsius. Retains full activity in MOPS buffer between pH 6.5 and 10.0. Is irreversibly damaged below pH 6.5 in MOPS buffer, enzyme treated at pH 6.0 in MOPS buffer only regains 40% of its original activity after re-equilibration at pH 7.2. When treated at pH 5.2 or 10.1 in 20 mM potassium phosphate/2 mM DTT buffer activity is lost completely, but is recovered within 17 minutes following readjustment to pH 7.4. Ref.2 Ref.3 Ref.4 Temperature dependence: Optimum temperature is 45 degrees Celsius. Activity is lost after 5 minutes incubation at 60 degrees Celsius, but one-fifth of this activity is restored after cooling to 45 degrees Celsius.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	4-chlorobenzoyl-CoA dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2 Ref.3
	Chain	2 – 276	275	4-chlorobenzoyl coenzyme A dehalogenase-1 Ref.2 Ref.3		PRO_0000401145
Regions
	Region	66 – 71	6	Substrate binding By similarity
Sites
	Active site	93	1	Proton acceptor By similarity UniProtKB A5JTM5
	Active site	148	1	Nucleophile By similarity UniProtKB A5JTM5
	Binding site	117	1	Substrate; via amide nitrogen By similarity
	Binding site	261	1	Substrate; shared with oligomeric partner By similarity UniProtKB A5JTM5

Sequences

	Sequence		Length	Mass (Da)
	O85078 [UniParc]. Last modified October 1, 2000. Version 2. Checksum: E59F5CF36E8FD305	FASTA	276	29,899
	10 20 30 40 50 60 MSSNSDHHIS VEHTDGVATI RFTRPSKHNA ASGQLLLETL EALYRLESDD SVGAIVLTGE 70 80 90 100 110 120 GAVFSAGFDL EEVPMGPASE IQSHFRLKAL YYHAVIHMLA RIEKPTLAAI NGPAVGGGLG 130 140 150 160 170 180 MSLACDLAVC TDRATFLPAW MSIGIANDAS SSFYLPRIVG YRRAMEWLLT NRTLGADEAY 190 200 210 220 230 240 EWGVVNRVFS EADFQSRVGE IARQLAAAPT HLQGLVKNRI QEGSSETLES CTEHEVQNVI 250 260 270 ASVGHPHFAE RLAMFRSKEM RSSALAVDLD AVCGGR

References

[1]	"Isolation and characterization of IS1409, an insertion element of 4-chlorobenzoate-degrading Arthrobacter sp. strain TM1, and development of a system for transposon mutagenesis." Gartemann K.H., Eichenlaub R. J. Bacteriol. 183:3729-3736(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCIB 12013 / TM1.
[2]	"Purification and characterization of 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain 4-CB1." Crooks G.P., Copley S.D. Biochemistry 33:11645-11649(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-25, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: 4-CB1.
[3]	"The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1." Zhou L., Marks T.S., Poh R.P., Smith R.J., Chowdhry B.Z., Smith A.R. Biodegradation 15:97-109(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: NCIB 12013 / TM1.
[4]	"Structure and denaturation of 4-chlorobenzoyl coenzyme A dehalogenase from Arthrobacter sp. strain TM-1." Zhou L., Poh R.P., Marks T.S., Chowdhry B.Z., Smith A.R. Biodegradation 19:65-75(2008) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES. Strain: NCIB 12013 / TM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042490 Genomic DNA. Translation: AAF76241.1.
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ProteinModelPortal	O85078.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA01011; UER01021.
Family and domain databases
Gene3D	1.10.12.10. 1 hit.
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core_superfam. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBAD1_ARTSP

Accession

Primary (citable) accession number: O85078
Secondary accession number(s): Q04415

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	October 1, 2000
Last modified:	March 6, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program