Reviewed, UniProtKB/Swiss-Prot O85071 (LEU3_BUCDN)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:

leuB

Encoded on

Plasmid pLeu-Dn (pBDn1)

Organism

Buchnera aphidicola subsp. Diuraphis noxia

Taxonomic identifier

118101 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	3-isopropylmalate dehydrogenase		PRO_0000083657
Regions
	Nucleotide binding	78 – 91	14	NAD By similarity
	Nucleotide binding	285 – 297	13	NAD By similarity
Sites
	Metal binding	227	1	Magnesium or manganese By similarity
	Metal binding	251	1	Magnesium or manganese By similarity
	Metal binding	255	1	Magnesium or manganese By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Site	145	1	Important for catalysis By similarity
	Site	195	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O85071-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C4B5F8B3E4F00735	FASTA	363	41,056
	10 20 30 40 50 60 MHKQYHIAVL PGDGIGPEVM QEAYKILQVL REHFSLFIKT KEFDIGGIAI DNHGIALPKK 70 80 90 100 110 120 TLIGCENSDA ILLGSIGGKK WDTLPINERP ERASLLPLRK HFNFFCNLRP SNLYKELNFL 130 140 150 160 170 180 SPLRNDIVKH GFDILCVREL TGGIYFGKPR GRVTKKKLMY AFDTEIYYKF EIVRIAHLAF 190 200 210 220 230 240 KLARSRKHKL CSIDKANVLE SSILWREVVE EVSKEYPDVI LSHLYIDNVC MQIIKDPNQF 250 260 270 280 290 300 DVLLCSNLFG DIISDECAMI TGSIGMLPSA SLNEKNFGLY EPAGGSAPDI QGKNIANPIA 310 320 330 340 350 360 QILSLSMLIR YSMNLNKIAN KIDNAVINVL KKGYKTMDIS KDQNYLKTNE MGDVIADFLK RDK

References

[1]

"Genetic characterization of plasmids containing genes encoding enzymes of leucine biosynthesis in endosymbionts (Buchnera) of aphids." Baumann L., Baumann P., Moran N.A., Sandstroem J.P., Thao M.L. J. Mol. Evol. 48:77-85(1999) [PubMed: 9873079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF041837 Genomic DNA. Translation: AAD12601.1.
RefSeq	NP_047188.1.
3D structure databases
HSSP	HSSP built from PDB template 1CNZ based on UniProtKB P37412.
ModBase	Search...
Genome annotation databases
GeneID	1246503.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BUCDN

Accession

Primary (citable) accession number: O85071

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1998
Last modified:	November 4, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)