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O85066 (LEUD_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase small subunit

EC=4.2.1.33
Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase
Gene names
Name:leuD
Ordered Locus Names:BUsg_PL7
Encoded onPlasmid pLeu-Sg (pBSg1)
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01031

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01031

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01031

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01031

Sequence similarities

Belongs to the LeuD family. LeuD type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
   Molecular functionLyase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological_processleucine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_component3-isopropylmalate dehydratase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function3-isopropylmalate dehydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2072073-isopropylmalate dehydratase small subunit HAMAP-Rule MF_01031
PRO_0000141803

Sequences

Sequence LengthMass (Da)Tools
O85066 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 0231CC4A8E795753

FASTA20724,229
        10         20         30         40         50         60 
MFKFIEHTGV VVPLNISNID TDAIIPKQFL KKVNKIGFGK YLFHDWRFLD KNQLKINPNF 

        70         80         90        100        110        120 
ILNKSTYKNA SILLTRENFG CGSSREHAVW SLLDYGFKVI IASSFSDIFY SNSFNNKLLL 

       130        140        150        160        170        180 
IVLENEDIDY LFDLVNTKIG LSFNVSLIHK KITVDNLDIP FQIDDFQRLC LVNNWDNNDL 

       190        200 
TMKIDHKIKL YEKNIFSFLL KREKFTS 

« Hide

References

[1]"Genetic characterization of plasmids containing genes encoding enzymes of leucine biosynthesis in endosymbionts (Buchnera) of aphids."
Baumann L., Baumann P., Moran N.A., Sandstroem J.P., Thao M.L.
J. Mol. Evol. 48:77-85(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF041836 Genomic DNA. Translation: AAD12596.1.
RefSeqNP_047183.1. NC_001910.1.

3D structure databases

ProteinModelPortalO85066.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2648304.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAHAVWALI.
ProtClustDBPRK01641.

Enzyme and pathway databases

UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.20.19.10. 1 hit.
HAMAPMF_01031. LeuD_type1.
InterProIPR004431. 3-IsopropMal_deHydase_ssu.
IPR015937. Acoase/IPM_deHydtase.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00694. Aconitase_C. 1 hit.
[Graphical view]
SUPFAMSSF52016. SSF52016. 1 hit.
TIGRFAMsTIGR00171. leuD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLEUD_BUCAP
AccessionPrimary (citable) accession number: O85066
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names