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Protein

3-isopropylmalate dehydratase small subunit

Gene

leuD

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.By similarity

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase small subunit (EC:4.2.1.33)
Alternative name(s):
Alpha-IPM isomerase
Short name:
IPMI
Isopropylmalate isomerase
Gene namesi
Name:leuD
Ordered Locus Names:BUsg_PL7
Encoded oniPlasmid pLeu-Sg (pBSg1)0 Publication
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Plasmid pLeu-Sg

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001418031 – 2073-isopropylmalate dehydratase small subunitAdd BLAST207

Proteomic databases

PRIDEiO85066

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.By similarity

Structurei

3D structure databases

ProteinModelPortaliO85066
SMRiO85066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LeuD family. LeuD type 1 subfamily.Curated

Phylogenomic databases

OMAiAFTTHTG

Family and domain databases

CDDicd01577 IPMI_Swivel, 1 hit
Gene3Di3.20.19.10, 1 hit
HAMAPiMF_01031 LeuD_type1, 1 hit
InterProiView protein in InterPro
IPR004431 3-IsopropMal_deHydase_ssu
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
IPR033940 IPMI_Swivel
PfamiView protein in Pfam
PF00694 Aconitase_C, 1 hit
TIGRFAMsiTIGR00171 leuD, 1 hit

Sequencei

Sequence statusi: Complete.

O85066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFIEHTGV VVPLNISNID TDAIIPKQFL KKVNKIGFGK YLFHDWRFLD
60 70 80 90 100
KNQLKINPNF ILNKSTYKNA SILLTRENFG CGSSREHAVW SLLDYGFKVI
110 120 130 140 150
IASSFSDIFY SNSFNNKLLL IVLENEDIDY LFDLVNTKIG LSFNVSLIHK
160 170 180 190 200
KITVDNLDIP FQIDDFQRLC LVNNWDNNDL TMKIDHKIKL YEKNIFSFLL

KREKFTS
Length:207
Mass (Da):24,229
Last modified:November 1, 1998 - v1
Checksum:i0231CC4A8E795753
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041836 Genomic DNA Translation: AAD12596.1
RefSeqiNP_047183.1, NC_001910.1

Genome annotation databases

GeneIDi2648304

Similar proteinsi

Entry informationi

Entry nameiLEUD_BUCAP
AccessioniPrimary (citable) accession number: O85066
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid
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Main funding by: National Institutes of Health