O85045 (ALR_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Synonyms:	dal
Ordered Locus Names:	lmo0886

Organism

Listeria monocytogenes

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Alanine racemase HAMAP MF_01201		PRO_0000114534
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	263	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	192	1	N → K in AAC31362. Ref.1
	Sequence conflict	298	1	G → D in AAC31362. Ref.1
	Sequence conflict	301	1	L → P in AAC31362. Ref.1
	Sequence conflict	338	1	I → V in AAC31362. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O85045 [UniParc]. Last modified December 5, 2001. Version 2. Checksum: 589812C3014193F7	FASTA	368	40,987
	10 20 30 40 50 60 MVTGWHRPTW IEIDRAAIRE NIKNEQNKLP ESVDLWAVVK ANAYGHGIIE VARTAKEAGA 70 80 90 100 110 120 KGFCVAILDE ALALREAGFQ DDFILVLGAT RKEDANLAAK NHISLTVFRE DWLENLTLEA 130 140 150 160 170 180 TLRIHLKVDS GMGRLGIRTT EEARRIEATS TNDHQLQLEG IYTHFATADQ LETSYFEQQL 190 200 210 220 230 240 AKFQTILTSL KNRPTYVHTA NSAASLLQPQ IGFDAIRFGI SMYGLTPSTE IKTSLPFELK 250 260 270 280 290 300 PALALYTEMV HVKELAPGDS VSYGATYTAT EREWVATLPI GYADGLIRHY SGFHVLVGGE 310 320 330 340 350 360 LAPIIGRVCM DQTIIKLPRE FQTGSKVTII GKDHGNTITA DDAAQYLDTI NYEVTCLLNE RIPRKYIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pathogenicity and immunogenicity of a Listeria monocytogenes strain that requires D-alanine for growth." Thompson R.J., Bouwer H.G.A., Portnoy D.A., Frankel F.R. Infect. Immun. 66:3552-3561(1998) [PubMed: 9673233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 10403S / Serovar 1/2a.
[2]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF038438 Genomic DNA. Translation: AAC31362.1. AL591977 Genomic DNA. Translation: CAC98964.1.
PIR	AF1185.
RefSeq	NP_464412.1. NC_003210.1.
3D structure databases
ProteinModelPortal	O85045.
SMR	O85045. Positions 4-367.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	986294.
GenomeReviews	Gene locus lmo0886 in contig AL591824_GR.
KEGG	lmo:lmo0886.
PATRIC	20310856. VBILisMon69206_0911.
Organism-specific databases
GenoList	LMO0886.
Phylogenomic databases
HOGENOM	HBG712172.
OMA	TRKEDAN.
ProtClustDB	CLSK564147.
Enzyme and pathway databases
BioCyc	LMON169963:LMO0886-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LISMO

Accession

Primary (citable) accession number: O85045

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 5, 2001
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program