O84990 (RIBF_RHOOP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:

ribF

Organism

Rhodococcus opacus (Nocardia opaca)

Taxonomic identifier

37919 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the RibF family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Putative riboflavin biosynthesis protein RibF		PRO_0000194142

Sequences

	Sequence		Length	Mass (Da)
	O84990 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 7869F5B3D244B56A	FASTA	248	26,703
	10 20 30 40 50 60 MPVAEATTAT GLASHNGRQS GVAIGAFDGV HLGHRQVLVG CDTVLTFDPH PMHVLAPRPA 70 80 90 100 110 120 LRLLSDRRTK LRKLEALGIR RVAFIPFDEG WSRVTADDFV ERVVIDRLHA SRVSVGANFR 130 140 150 160 170 180 FGAHGVGTPE TFNNYQSLQT RVVQLVQRGP GAEPISSTRI RKLVAAGNIE DAIDLLGGPF 190 200 210 220 230 240 SLPAVAVSES RLVIADEFAL PAPGLYSAQV DGNAVSLRTF IEGVVELTHP ASLQRDTTVE VSFLNRMA

References

[1]	"Characterization of a maleylacetate reductase encoding region from Rhodococcus opacus 1CP." Seibert V., Kourbatova E.M., Golovleva L.A., Schloemann M. J. Bacteriol. 180:3503-3508(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1CP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030176 Genomic DNA. Translation: AAC38800.1.
3D structure databases
ProteinModelPortal	O84990.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIBF_RHOOP

Accession

Primary (citable) accession number: O84990

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program