Reviewed, UniProtKB/Swiss-Prot O84970 (NUOB_RHOCA)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B
    NDH-1 subunit B

Gene names

Name:

nuoB

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	NADH-quinone oxidoreductase subunit B		PRO_0000118777
Sites
	Metal binding	56	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	57	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	151	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O84970-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 1FD2E4879646AD85	FASTA	177	19,509
	10 20 30 40 50 60 MGVMTGSNTA AVDMDVAAAA LNRDLQDKGF LLTTAEDIIN WARNGSLHWM TFGLACCAVE 70 80 90 100 110 120 MMHTAMPRYD VERYGFAPRA SPRQSDVMIV AGTLTNKMAP ALRKVYDQMP EPRYVISMGS 130 140 150 160 170 CANGGGYYHY SYSVVRGCDR IVPVDIYVPG CPPSAEALMY GILQLQRKIR RTGTLVR

References

[1]	Dupuis A., Issartel J.P. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33303 / B10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF029365 Genomic DNA. Translation: AAC24986.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOB_RHOCA

Accession

Primary (citable) accession number: O84970

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)