O84955 (BLO20_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase OXA-20
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:	bla
Synonyms:	oxa20

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is an oxacillin-hydrolyzing beta-lactamase.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited by clavulanic acid.
Sequence similarities	Belongs to the class-D beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro peptidoglycan-based cell wall biogenesis Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 266	245	Beta-lactamase OXA-20		PRO_0000017034
Regions
	Region	210 – 212	3	Substrate binding By similarity
Sites
	Active site	72	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	75	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84955 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 8E7270EA924F2FB9	FASTA	266	30,449
	10 20 30 40 50 60 MIIRFLALLF SAVVLVSLGH AQEKTHESSN WGKYFSDFNA KGTIVVVDER TNGNSTSVYN 70 80 90 100 110 120 ESRAQQRYSP ASTFKIPHTL FALDAGAVRD EFHVFRWDGA KRSFAGHNQD QNLRSAMRNS 130 140 150 160 170 180 TVWVYQLFAK EIGENKARSY LEKLNYGNAD PSTKSGDYWI DGNLAISANE QISILKKLYR 190 200 210 220 230 240 NELPFRVEHQ RLVKDLMIVE AKRDWILRAK TGWDGQMGWW VGWVEWPTGP VFFALNIDTP 250 260 NRMEDLHKRE AIARAILQSV NALPPN

References

[1]

"Molecular characterization of OXA-20, a novel class D beta-lactamase, and its integron from Pseudomonas aeruginosa." Naas T., Sougakoff W., Casetta A., Nordmann P. Antimicrob. Agents Chemother. 42:2074-2083(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Mus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF024602 Genomic DNA. Translation: AAC23554.1.
3D structure databases
ProteinModelPortal	O84955.
SMR	O84955. Positions 25-265.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR002137. Beta-lactam_class-D_AS. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00337. BETA_LACTAMASE_D. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLO20_PSEAI

Accession

Primary (citable) accession number: O84955

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program