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O84917 (RBL2_THIK1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase

Short name=RuBisCO
EC=4.1.1.39
Gene names
Name:cbbM
Ordered Locus Names:Tint_1655
OrganismThiomonas intermedia (strain K12) (Thiobacillus intermedius) [Complete proteome] [HAMAP]
Taxonomic identifier75379 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesThiomonas

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01339

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01339

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01339

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01339

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01339

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In contrast to form I RuBisCO, the form II RuBisCO are composed solely of large subunits By similarity.

Sequence similarities

Belongs to the RuBisCO large chain family. Type II subfamily.

Caution

No expression of this protein has been seen during autotrophic and mixotrophic growth, however the protein is probably expressed under other conditions. In a related bacteria (T.neapolitanus) expression has been seen when the form I enzyme has been knocked out.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 461461Ribulose bisphosphate carboxylase HAMAP-Rule MF_01339
PRO_0000062669

Sites

Active site1681Proton acceptor By similarity
Active site2891Proton acceptor By similarity
Metal binding1931Magnesium; via carbamate group By similarity
Metal binding1951Magnesium By similarity
Metal binding1961Magnesium By similarity
Binding site1131Substrate; in homodimeric partner By similarity
Binding site1701Substrate By similarity
Binding site2901Substrate By similarity
Binding site3231Substrate By similarity
Binding site3701Substrate By similarity
Site3311Transition state stabilizer By similarity

Amino acid modifications

Modified residue1931N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
O84917 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: D470D17F356800E2

FASTA46150,818
        10         20         30         40         50         60 
MAHDQSSRYA NLDLKESDLI AGGKHILVAY KMKPKAGYDY LATAAHFAAE SSTGTNVEVS 

        70         80         90        100        110        120 
TTDDFTKGVD ALVYFIDEAT EDMRIAYPIE LFDRNVIDGR FMIVSFLTLV IGNNQGMGDV 

       130        140        150        160        170        180 
EYGKMIDFYV PERAIQMFDG PATDISNLWR ILGRPIKDGG YIAGTIIKPK LGLRPEPFAQ 

       190        200        210        220        230        240 
AAYQFWLGGD FIKNDEPQGN QVFAPVKKVI PLVYDAMKRA QDETGEAKLF SMNITADDYH 

       250        260        270        280        290        300 
EMCARADFAL EVFGPDADKL AFLVDGYVGG PGMVTTARRQ YPNQYLHYHR AGHGAITSPS 

       310        320        330        340        350        360 
SKRGYTAFVL AKMSRLQGAS GIHVGTMGYG KMEGEGDDRN IAYMIERDEC QGPVYFQKWY 

       370        380        390        400        410        420 
GMKPTTPIIS GGMNALRLPG FFENLGHGNV INTAGGGSYG HIDSPAAGAK SLRQAYECWK 

       430        440        450        460 
AGADPIEYAK EHKEFARAFE SFPGDADKLF PGWRDKLGVH K 

« Hide

References

« Hide 'large scale' references
[1]"A form II Rubisco gene and associated genes in Thiobacillus intermedius."
Shively J.M., Soyer F.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of Thiomonas intermedia K12."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kerfeld C.A., Cannon G.C., Heinhorst S., Woyke T.
Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"Cloning and expression of the D-ribulose-1,5-bisphosphate carboxylase/oxygenase form II gene from Thiobacillus intermedius in Escherichia coli."
Stoner M.T., Shively J.M.
FEMS Microbiol. Lett. 107:287-292(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPRESSION IN T.INTERMEDIUS.
[4]Shively J.M.
Unpublished observations (OCT-2005)
Cited for: PROBABLE EXPRESSION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012127 Genomic DNA. Translation: AAC24964.1.
CP002021 Genomic DNA. Translation: ADG31025.1.
RefSeqYP_003643355.1. NC_014153.1.

3D structure databases

ProteinModelPortalO84917.
SMRO84917. Positions 4-459.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADG31025; ADG31025; Tint_1655.
GeneID9152388.
KEGGtin:Tint_1655.
PATRIC38293853. VBIThiInt85915_1685.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000230831.
KOK01601.
ProtClustDBPRK13475.

Enzyme and pathway databases

BioCycTINT75379:GH6C-1675-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01339. RuBisCO_L_type2.
InterProIPR020871. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBL2_THIK1
AccessionPrimary (citable) accession number: O84917
Secondary accession number(s): D5X1M9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families