Reviewed, UniProtKB/Swiss-Prot O84874 (GLGB_CHLTR)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	CT_866

Organism

Chlamydia trachomatis [Complete proteome] [HAMAP]

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188694
Sites
	Active site	294	1	By similarity
	Active site	329	1	By similarity
	Active site	334	1	By similarity
	Active site	397	1	By similarity
	Active site	399	1	By similarity
	Active site	452	1	By similarity
	Active site	519	1	By similarity
	Active site	520	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84874-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 89E05A005752C1BE	FASTA	738	84,651
	10 20 30 40 50 60 MDPFFLNTQH VELLVSGKQS SPQDLLGIVS ESLNQDRIVL FRPGAETVFV ELRGKIQQAE 70 80 90 100 110 120 SHHSGIFSLP VMKGISPQDY RVYHQNGLLA HDPYAFPLLW GEIDSFLFHE GTHQRIYERM 130 140 150 160 170 180 GAIPCEIDGV PGVRFIVWAP HAQRVSVIGD FNGWHGLVNP LHKVSDQGVW ELFVPGLTAG 190 200 210 220 230 240 ACYKWEMVTE SGQVLIKSDP YGKFFGPPPW SVSVVIDDSY EWTDSEWLEE RIKKTEGPMN 250 260 270 280 290 300 IYEVHVGSWR WQEGQPLNYK ELADQLALYC KQMHYTHVEL LPVTEHPLNE SWGYQTTGYY 310 320 330 340 350 360 APTSRYGSFE DLQYFIDTMH QHGIGVILDW VPGHFPIDSF AMSGFDGTPL YEYTRNPSPL 370 380 390 400 410 420 HPHWHTYTFD YAKPEVCNFL LGSVLFWIDK MHVDGIRVDA VSSMLYLDYG RYAGEWVPNR 430 440 450 460 470 480 YGGRENLDAI RFLQQFNTVI HEKYPGVLTF AEESTTFPKI TVSVEEGGLG FDYKWNMGWM 490 500 510 520 530 540 HDTLHYFEKD FPYRPYHQSD LTFPQWYAFS ERFLLPFSHD EVVHGKRSLI GKMPGDAWRQ 550 560 570 580 590 600 FAQLRLLLGY QICQPGKKLL FMGGEFGQGR EWSPGRELDW ELLDISYHQG VHLCSQELNA 610 620 630 640 650 660 LYVQSPQLWQ ADHLPSSFRW VDFSDVRNGV VAYLRFADAD AKKALLCVHH FGVGYFPHYL 670 680 690 700 710 720 LPILPLESCD LLMNTDDTRF GGSGKGFREP EILTPEIARQ EREAAGLIEA DDESGPDCWG 730 LDIELPPSAT LIFSVTLQ

References

[1]

"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001273 Genomic DNA. Translation: AAC68464.1.
PIR	A71462.
RefSeq	NP_220388.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	884667.
GenomeReviews	Gene locus CT_866 in contig AE001273_GR.
KEGG	ctr:CT866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O84874.
OMA	GFDWSVA.
Enzyme and pathway databases
BioCyc	CTRA315277:CT866-MON.
BRENDA	2.4.1.18. 108.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_CHLTR

Accession

Primary (citable) accession number: O84874

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)