Reviewed, UniProtKB/Swiss-Prot O84863 (FUMC_CHLTR)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	CT_855

Organism

Chlamydia trachomatis [Complete proteome] [HAMAP]

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161269
Regions
	Region	126 – 129	4	B site By similarity
	Region	136 – 138	3	Substrate binding By similarity
Sites
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84863-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 428E3DA10A839853	FASTA	463	50,339
	10 20 30 40 50 60 MRQENDSLGI VLVPEDKLFG AQTGRSQEFF SYGKESMPLE IIHALVKIKK CAAKANGDLG 70 80 90 100 110 120 CLDAKRRDMI VAATDEILSG EFDEHFPLKV WQTGSGTQSN MNVNEVIANL AIQRHGGELG 130 140 150 160 170 180 SKHPVHPNDH VNKSQSSNDV FPTAMHIAAV QSIKGSLIPA LEHLKKVIDA KALEFARDIK 190 200 210 220 230 240 IGRTHLMDAV PMTLGQEFSG YSCQLHNCLE RIGFSLTHLY ELAIGGTAIG TGLNVPEGFV 250 260 270 280 290 300 EKVIQYLRRE TGEPFVPASN YFAALSNHDA LVQAHGSLTV LACALVKIAT DLSFLGSGPR 310 320 330 340 350 360 CGLGEIFFPE NEPGSSIMPG KINPTQSEAL QMVCSQVIGN NQSIIFSGTK GNFELNVMKP 370 380 390 400 410 420 VIIYDFLQSV NLLAGAMRSF ADYFVCGLKV NKGQLQQNVE RSLMLVTALA PVLGYDKCSK 430 440 450 460 IALKAFHENL SLKEACVSLG FLSEKEFDEH VIPGLMVGNR GHE

References

[1]

"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001273 Genomic DNA. Translation: AAC68452.1.
PIR	H71462.
RefSeq	NP_220377.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
ModBase	Search...
2-D gel databases
PHCI-2DPAGE	O84863.
Genome annotation databases
GeneID	884656.
GenomeReviews	Gene locus CT_855 in contig AE001273_GR.
KEGG	ctr:CT855.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O84863.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	CTRA315277:CT855-MON.
BRENDA	4.2.1.2. 108.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_CHLTR

Accession

Primary (citable) accession number: O84863

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)