O84859 (AMPM_CHLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	CT_851

Organism

Chlamydia trachomatis

Taxonomic identifier

813 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Methionine aminopeptidase		PRO_0000148934
Sites
	Metal binding	135	1	Cobalt 1 By similarity
	Metal binding	146	1	Cobalt 1 By similarity
	Metal binding	146	1	Cobalt 2 By similarity
	Metal binding	209	1	Cobalt 2 By similarity
	Metal binding	241	1	Cobalt 2 By similarity
	Metal binding	273	1	Cobalt 1 By similarity
	Metal binding	273	1	Cobalt 2 By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	216	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O84859 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: DCC01BC0157C0A89	FASTA	291	32,641
	10 20 30 40 50 60 MKRNDPCWCG SNKKWKHCHY PTKPERPLDN LRQLYASRYD IIIKTPEQIE KIRKACQVTA 70 80 90 100 110 120 HILDALCEAA KEGVTTNELD LLSRELHKRH NAIPAPLNYG HPPFPKTICT SLNEVICHGI 130 140 150 160 170 180 PNDIPLQNGD IMNIDVSCIV DGFYGDCSRM VMIGEVSEIK RKVCEASLEA LNAAISILEP 190 200 210 220 230 240 NLPLYEIGEV IENCAAKYGF SVVDQFVGHG VGVKFHENPF VAHHRNSCKI PLAPGMIFTI 250 260 270 280 290 EPMINVGKKE GFIDPINHWE ARTCDHQPSA QWEHAILITD SGCEVLTLLD K

References

[1]	"Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis." Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W. Science 282:754-759(1998) [PubMed: 9784136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: D/UW-3/Cx.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001273 Genomic DNA. Translation: AAC68448.1.
PIR	D71462.
RefSeq	NP_220373.1. NC_000117.1.
3D structure databases
ProteinModelPortal	O84859.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
2D gel databases
PHCI-2DPAGE	O84859.
Siena-2DPAGE	O84859.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	884653.
GenomeReviews	Gene locus CT_851 in contig AE001273_GR.
KEGG	ctr:CT851.
PATRIC	20381424. VBIChlTra43535_0940.
Phylogenomic databases
HOGENOM	HBG299384.
OMA	FHENPYV.
ProtClustDB	PRK12318.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. IPR004027. SEC_C_motif. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. PF02810. SEC-C. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_CHLTR

Accession

Primary (citable) accession number: O84859

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program