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O84834

- RIR1_CHLTR

UniProt

O84834 - RIR1_CHLTR

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Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdA, CT_827
Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei442 – 4421Substrate By similarity
Sitei458 – 4581Important for hydrogen atom transfer By similarity
Sitei465 – 4651Allosteric effector binding By similarity
Binding sitei486 – 4861Substrate; via amide nitrogen By similarity
Sitei495 – 4951Allosteric effector binding By similarity
Active sitei670 – 6701Proton acceptor By similarity
Active sitei672 – 6721Cysteine radical intermediate By similarity
Active sitei674 – 6741Proton acceptor By similarity
Sitei687 – 6871Important for hydrogen atom transfer By similarity
Sitei990 – 9901Important for electron transfer By similarity
Sitei991 – 9911Important for electron transfer By similarity
Sitei1043 – 10431Interacts with thioredoxin/glutaredoxin By similarity
Sitei1046 – 10461Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15783.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:CT_827
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000431: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi458 ↔ 687Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi272561.CT827.

Structurei

3D structure databases

ProteinModelPortaliO84834.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 111103ATP-cone 1
Add
BLAST
Domaini118 – 219102ATP-cone 2
Add
BLAST
Domaini237 – 32791ATP-cone 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni457 – 4582Substrate binding By similarity
Regioni670 – 6745Substrate binding By similarity
Regioni857 – 8615Substrate binding By similarity

Sequence similaritiesi

Contains 3 ATP-cone domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0209.
KOiK00525.
OMAiDRYFLHI.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84834-1 [UniParc]FASTAAdd to Basket

« Hide

MVDLQEKQCT IVKRNGMFVP FDRNRIFQAL EAAFRDTRRI DDHMPLPEDL     50
ESSIRSITHQ VVKEVVQKIT DGQVVTVERI QDMVESQLYV NGLQDVARDY 100
IVYRDDRKAH RKKSWQSLSV VRRCGTVVHF NPMKISAALE KAFRATDKTE 150
GMTPSSVREE INALTQNIVA EIEECCPQQD RRIDIEKIQD IVEQQLMVVG 200
HYAVAKNYIL YREARARVRD NREEDGSTEK TIAEEAVEVL SKDGSTYTMT 250
HSQLLAHLAR ACSRFPETTD AALLTDMAFA NFYSGIKESE VVLACIMAAR 300
ANIEKEPDYA FVAAELLLDV VYKEALGKSK YAEDLEQAHR DHFKRYIAEG 350
DTYRLNAELK HLFDLDALAD AMDLSRDLQF SYMGIQNLYD RYFNHHEGCR 400
LETPQIFWMR VAMGLALNEQ DKTSWAITFY NLLSTFRYTP ATPTLFNSGM 450
RHSQLSSCYL STVQDNLVNI YKVIADNAML SKWAGGIGND WTAIRATGAL 500
IKGTNGRSQG VIPFIKVTND TAVAVNQGGK RKGAVCVYLE VWHLDYEDFL 550
ELRKNTGDER RRAHDVNIAS WIPDLFFKRL QQKGTWTLFS PDDVPGLHDA 600
YGEEFERLYE EYERKVDTGE IRLFKKVEAE DLWRKMLSML FETGHPWMTF 650
KDPSNIRSAQ DHKGVVRCSN LCTEILLNCS ETETAVCNLG SINLVQHIVG 700
DGLDEEKLSE TISIAVRMLD NVIDINFYPT KEAKEANFAH RAIGLGVMGF 750
QDALYKLDIS YASQEAVEFA DYSSELISYY AIQASCLLAK ERGTYSSYKG 800
SKWDRGLLPI DTIQLLANYR GEANLQMDTS SRKDWEPIRS LVKEHGMRHC 850
QLMAIAPTAT ISNIIGVTQS IEPTYKHLFV KSNLSGEFTI PNVYLIEKLK 900
KLGIWDADML DDLKYFDGSL LEIERIPDHL KHIFLTAFEI EPEWIIECAS 950
RRQKWIDMGQ SLNLYLAQPD GKKLSNMYLT AWKKGLKTTY YLRSSSATTV 1000
EKSFVDINKR GIQPRWMKNK SASAGIIVER AKKAPVCSLE EGCEACQ 1047
Length:1,047
Mass (Da):119,421
Last modified:May 30, 2000 - v2
Checksum:iA3658C187B4668AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001273 Genomic DNA. Translation: AAC68424.2.
PIRiD71466.
RefSeqiNP_220348.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC68424; AAC68424; CT_827.
GeneIDi884629.
KEGGictr:CT_827.
PATRICi20381366. VBIChlTra43535_0913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001273 Genomic DNA. Translation: AAC68424.2 .
PIRi D71466.
RefSeqi NP_220348.1. NC_000117.1.

3D structure databases

ProteinModelPortali O84834.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272561.CT827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC68424 ; AAC68424 ; CT_827 .
GeneIDi 884629.
KEGGi ctr:CT_827.
PATRICi 20381366. VBIChlTra43535_0913.

Phylogenomic databases

eggNOGi COG0209.
KOi K00525.
OMAi DRYFLHI.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci MetaCyc:MONOMER-15783.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.

Entry informationi

Entry nameiRIR1_CHLTR
AccessioniPrimary (citable) accession number: O84834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 14, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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