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O84834

- RIR1_CHLTR

UniProt

O84834 - RIR1_CHLTR

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei442 – 4421SubstrateBy similarity
Sitei458 – 4581Important for hydrogen atom transferBy similarity
Sitei465 – 4651Allosteric effector bindingBy similarity
Binding sitei486 – 4861Substrate; via amide nitrogenBy similarity
Sitei495 – 4951Allosteric effector bindingBy similarity
Active sitei670 – 6701Proton acceptorBy similarity
Active sitei672 – 6721Cysteine radical intermediateBy similarity
Active sitei674 – 6741Proton acceptorBy similarity
Sitei687 – 6871Important for hydrogen atom transferBy similarity
Sitei990 – 9901Important for electron transferBy similarity
Sitei991 – 9911Important for electron transferBy similarity
Sitei1043 – 10431Interacts with thioredoxin/glutaredoxinBy similarity
Sitei1046 – 10461Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15783.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:CT_827
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000431: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi458 ↔ 687Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi272561.CT827.

Structurei

3D structure databases

ProteinModelPortaliO84834.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 111103ATP-cone 1PROSITE-ProRule annotationAdd
BLAST
Domaini118 – 219102ATP-cone 2PROSITE-ProRule annotationAdd
BLAST
Domaini237 – 32791ATP-cone 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni457 – 4582Substrate bindingBy similarity
Regioni670 – 6745Substrate bindingBy similarity
Regioni857 – 8615Substrate bindingBy similarity

Sequence similaritiesi

Contains 3 ATP-cone domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0209.
InParanoidiO84834.
KOiK00525.
OMAiDRYFLHI.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84834-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVDLQEKQCT IVKRNGMFVP FDRNRIFQAL EAAFRDTRRI DDHMPLPEDL
60 70 80 90 100
ESSIRSITHQ VVKEVVQKIT DGQVVTVERI QDMVESQLYV NGLQDVARDY
110 120 130 140 150
IVYRDDRKAH RKKSWQSLSV VRRCGTVVHF NPMKISAALE KAFRATDKTE
160 170 180 190 200
GMTPSSVREE INALTQNIVA EIEECCPQQD RRIDIEKIQD IVEQQLMVVG
210 220 230 240 250
HYAVAKNYIL YREARARVRD NREEDGSTEK TIAEEAVEVL SKDGSTYTMT
260 270 280 290 300
HSQLLAHLAR ACSRFPETTD AALLTDMAFA NFYSGIKESE VVLACIMAAR
310 320 330 340 350
ANIEKEPDYA FVAAELLLDV VYKEALGKSK YAEDLEQAHR DHFKRYIAEG
360 370 380 390 400
DTYRLNAELK HLFDLDALAD AMDLSRDLQF SYMGIQNLYD RYFNHHEGCR
410 420 430 440 450
LETPQIFWMR VAMGLALNEQ DKTSWAITFY NLLSTFRYTP ATPTLFNSGM
460 470 480 490 500
RHSQLSSCYL STVQDNLVNI YKVIADNAML SKWAGGIGND WTAIRATGAL
510 520 530 540 550
IKGTNGRSQG VIPFIKVTND TAVAVNQGGK RKGAVCVYLE VWHLDYEDFL
560 570 580 590 600
ELRKNTGDER RRAHDVNIAS WIPDLFFKRL QQKGTWTLFS PDDVPGLHDA
610 620 630 640 650
YGEEFERLYE EYERKVDTGE IRLFKKVEAE DLWRKMLSML FETGHPWMTF
660 670 680 690 700
KDPSNIRSAQ DHKGVVRCSN LCTEILLNCS ETETAVCNLG SINLVQHIVG
710 720 730 740 750
DGLDEEKLSE TISIAVRMLD NVIDINFYPT KEAKEANFAH RAIGLGVMGF
760 770 780 790 800
QDALYKLDIS YASQEAVEFA DYSSELISYY AIQASCLLAK ERGTYSSYKG
810 820 830 840 850
SKWDRGLLPI DTIQLLANYR GEANLQMDTS SRKDWEPIRS LVKEHGMRHC
860 870 880 890 900
QLMAIAPTAT ISNIIGVTQS IEPTYKHLFV KSNLSGEFTI PNVYLIEKLK
910 920 930 940 950
KLGIWDADML DDLKYFDGSL LEIERIPDHL KHIFLTAFEI EPEWIIECAS
960 970 980 990 1000
RRQKWIDMGQ SLNLYLAQPD GKKLSNMYLT AWKKGLKTTY YLRSSSATTV
1010 1020 1030 1040
EKSFVDINKR GIQPRWMKNK SASAGIIVER AKKAPVCSLE EGCEACQ
Length:1,047
Mass (Da):119,421
Last modified:May 30, 2000 - v2
Checksum:iA3658C187B4668AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC68424.2.
PIRiD71466.
RefSeqiNP_220348.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC68424; AAC68424; CT_827.
GeneIDi884629.
KEGGictr:CT_827.
PATRICi20381366. VBIChlTra43535_0913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC68424.2 .
PIRi D71466.
RefSeqi NP_220348.1. NC_000117.1.

3D structure databases

ProteinModelPortali O84834.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272561.CT827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC68424 ; AAC68424 ; CT_827 .
GeneIDi 884629.
KEGGi ctr:CT_827.
PATRICi 20381366. VBIChlTra43535_0913.

Phylogenomic databases

eggNOGi COG0209.
InParanoidi O84834.
KOi K00525.
OMAi DRYFLHI.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci MetaCyc:MONOMER-15783.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."
    Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.
    Science 282:754-759(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: D/UW-3/Cx.

Entry informationi

Entry nameiRIR1_CHLTR
AccessioniPrimary (citable) accession number: O84834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3