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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei442SubstrateBy similarity1
Sitei458Important for hydrogen atom transferBy similarity1
Sitei465Allosteric effector bindingBy similarity1
Binding sitei486Substrate; via amide nitrogenBy similarity1
Sitei495Allosteric effector bindingBy similarity1
Active sitei670Proton acceptorBy similarity1
Active sitei672Cysteine radical intermediateBy similarity1
Active sitei674Proton acceptorBy similarity1
Sitei687Important for hydrogen atom transferBy similarity1
Sitei990Important for electron transferBy similarity1
Sitei991Important for electron transferBy similarity1
Sitei1043Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei1046Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15783.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:CT_827
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872121 – 1047Ribonucleoside-diphosphate reductase subunit alphaAdd BLAST1047

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi458 ↔ 687Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO84834.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi272561.CT827.

Structurei

3D structure databases

ProteinModelPortaliO84834.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 111ATP-cone 1PROSITE-ProRule annotationAdd BLAST103
Domaini118 – 219ATP-cone 2PROSITE-ProRule annotationAdd BLAST102
Domaini237 – 327ATP-cone 3PROSITE-ProRule annotationAdd BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni457 – 458Substrate bindingBy similarity2
Regioni670 – 674Substrate bindingBy similarity5
Regioni857 – 861Substrate bindingBy similarity5

Sequence similaritiesi

Contains 3 ATP-cone domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
InParanoidiO84834.
KOiK00525.
OMAiFDYLGLQ.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDLQEKQCT IVKRNGMFVP FDRNRIFQAL EAAFRDTRRI DDHMPLPEDL
60 70 80 90 100
ESSIRSITHQ VVKEVVQKIT DGQVVTVERI QDMVESQLYV NGLQDVARDY
110 120 130 140 150
IVYRDDRKAH RKKSWQSLSV VRRCGTVVHF NPMKISAALE KAFRATDKTE
160 170 180 190 200
GMTPSSVREE INALTQNIVA EIEECCPQQD RRIDIEKIQD IVEQQLMVVG
210 220 230 240 250
HYAVAKNYIL YREARARVRD NREEDGSTEK TIAEEAVEVL SKDGSTYTMT
260 270 280 290 300
HSQLLAHLAR ACSRFPETTD AALLTDMAFA NFYSGIKESE VVLACIMAAR
310 320 330 340 350
ANIEKEPDYA FVAAELLLDV VYKEALGKSK YAEDLEQAHR DHFKRYIAEG
360 370 380 390 400
DTYRLNAELK HLFDLDALAD AMDLSRDLQF SYMGIQNLYD RYFNHHEGCR
410 420 430 440 450
LETPQIFWMR VAMGLALNEQ DKTSWAITFY NLLSTFRYTP ATPTLFNSGM
460 470 480 490 500
RHSQLSSCYL STVQDNLVNI YKVIADNAML SKWAGGIGND WTAIRATGAL
510 520 530 540 550
IKGTNGRSQG VIPFIKVTND TAVAVNQGGK RKGAVCVYLE VWHLDYEDFL
560 570 580 590 600
ELRKNTGDER RRAHDVNIAS WIPDLFFKRL QQKGTWTLFS PDDVPGLHDA
610 620 630 640 650
YGEEFERLYE EYERKVDTGE IRLFKKVEAE DLWRKMLSML FETGHPWMTF
660 670 680 690 700
KDPSNIRSAQ DHKGVVRCSN LCTEILLNCS ETETAVCNLG SINLVQHIVG
710 720 730 740 750
DGLDEEKLSE TISIAVRMLD NVIDINFYPT KEAKEANFAH RAIGLGVMGF
760 770 780 790 800
QDALYKLDIS YASQEAVEFA DYSSELISYY AIQASCLLAK ERGTYSSYKG
810 820 830 840 850
SKWDRGLLPI DTIQLLANYR GEANLQMDTS SRKDWEPIRS LVKEHGMRHC
860 870 880 890 900
QLMAIAPTAT ISNIIGVTQS IEPTYKHLFV KSNLSGEFTI PNVYLIEKLK
910 920 930 940 950
KLGIWDADML DDLKYFDGSL LEIERIPDHL KHIFLTAFEI EPEWIIECAS
960 970 980 990 1000
RRQKWIDMGQ SLNLYLAQPD GKKLSNMYLT AWKKGLKTTY YLRSSSATTV
1010 1020 1030 1040
EKSFVDINKR GIQPRWMKNK SASAGIIVER AKKAPVCSLE EGCEACQ
Length:1,047
Mass (Da):119,421
Last modified:May 30, 2000 - v2
Checksum:iA3658C187B4668AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC68424.2.
PIRiD71466.
RefSeqiNP_220348.1. NC_000117.1.
WP_009872213.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC68424; AAC68424; CT_827.
GeneIDi884629.
KEGGictr:CT_827.
PATRICi20381366. VBIChlTra43535_0913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC68424.2.
PIRiD71466.
RefSeqiNP_220348.1. NC_000117.1.
WP_009872213.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84834.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT827.

Proteomic databases

PRIDEiO84834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC68424; AAC68424; CT_827.
GeneIDi884629.
KEGGictr:CT_827.
PATRICi20381366. VBIChlTra43535_0913.

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
InParanoidiO84834.
KOiK00525.
OMAiFDYLGLQ.

Enzyme and pathway databases

UniPathwayiUPA00326.
BioCyciMetaCyc:MONOMER-15783.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_CHLTR
AccessioniPrimary (citable) accession number: O84834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 5, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.