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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi82Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi85Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi117Iron-sulfur (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Synonyms:nqr6
Ordered Locus Names:CT_740
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000744921 – 431Na(+)-translocating NADH-quinone reductase subunit FAdd BLAST431

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

STRINGi272561.CT740.

Structurei

3D structure databases

ProteinModelPortaliO84745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 1332Fe-2S ferredoxin-typeUniRule annotationAdd BLAST93
Domaini136 – 286FAD-binding FR-typeUniRule annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 413CatalyticAdd BLAST125

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JFA. Bacteria.
COG2871. LUCA.
InParanoidiO84745.
KOiK00351.
OMAiEEHGIIM.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O84745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWLSGLYSI FVASAAFCSL GLILVAVILL SRKFLIKVHP CKLKINNDDS
60 70 80 90 100
LTKTVDSGKT LLSSLLDSGI AIPSPCGGKA ACKQCKVRIT KNADEPLETD
110 120 130 140 150
RSTFSKQQLE QGWRLSCQTK VQHDLCLEVE ERYFNASSWE GTVVSNENVA
160 170 180 190 200
TFIKELVLSV DPSRPIPFKP GGYLQITVPP YKTNTSDWKQ TMDPQYYSDW
210 220 230 240 250
ETFHLFDQII DNLSLDTDSA NKAYSLASYP AELPLIKFNV RIATPPFVDQ
260 270 280 290 300
APDPTIPWGV CSSYIFSLKP GDKVMVSGPY GESFMKEDNR PVIFLIGGAG
310 320 330 340 350
SSFGRSHILD LLLNKHSDRE LTLWYGARSL KENIYQEEYE KLEKEFPNFH
360 370 380 390 400
YHLVLSQPLQ EDLDQGWDKN DPIKTNFLFK AFELGQLSHL PNPEDYLYYV
410 420 430
CGPALHNSSI LTLLDNYGVE RSSIVLDDFG S
Length:431
Mass (Da):48,513
Last modified:November 1, 1998 - v1
Checksum:i6F63E8A230B81777
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC68335.1.
PIRiF71476.
RefSeqiNP_220259.1. NC_000117.1.
WP_010725328.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC68335; AAC68335; CT_740.
GeneIDi884545.
KEGGictr:CT_740.
PATRICi20381160. VBIChlTra43535_0814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC68335.1.
PIRiF71476.
RefSeqiNP_220259.1. NC_000117.1.
WP_010725328.1. NC_000117.1.

3D structure databases

ProteinModelPortaliO84745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272561.CT740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC68335; AAC68335; CT_740.
GeneIDi884545.
KEGGictr:CT_740.
PATRICi20381160. VBIChlTra43535_0814.

Phylogenomic databases

eggNOGiENOG4108JFA. Bacteria.
COG2871. LUCA.
InParanoidiO84745.
KOiK00351.
OMAiEEHGIIM.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQRF_CHLTR
AccessioniPrimary (citable) accession number: O84745
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1998
Last modified: October 5, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.